[1]谢兴文△ 王春亮 徐世红 李鼎鹏 吕立桃.忍冬藤痛风颗粒对痛风性关节炎模型大鼠 MMP-3和LP-PLA2的影响[J].中国中医骨伤科杂志,2016,24(02):6-8.
　XIE Xingwen WANG Chunliang XU Shihong LI Dingpeng L Litao.Effect of Lonicera Japonica Gout Grain to MMP-3 and LP-PLA2 in Gouty Arthritis Model Rats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2016,24(02):6-8.
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忍冬藤痛风颗粒对痛风性关节炎模型大鼠 MMP-3和LP-PLA2的影响
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第24卷
期数:
2016年02期
页码:
6-8
栏目:
实验研究
出版日期:
2016-02-14

文章信息/Info

Title:
Effect of Lonicera Japonica Gout Grain to MMP-3 and LP-PLA2 in Gouty Arthritis Model Rats
文章编号:
1005-0205(2016)02-0006-03
作者:
谢兴文1△ 王春亮2 徐世红1 李鼎鹏2 吕立桃2
1.甘肃省中医院,甘肃省中医药研究院(兰州,730050)
2.甘肃中医药大学
△.通信作者 E-mail:827975272@qq.com
Author(s):
XIE Xingwen1△ WANG Chunliang2 XU Shihong1 LI Dingpeng2 LÜ Litao2
1.Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730050,China;
2.Gansu University of Chinese Medicine,Lanzhou 730050,China.
关键词:
忍冬藤痛风颗粒;  痛风性关节炎;  基质金属蛋白酶-3;  脂蛋白磷脂酶A2
Keywords:
lonicera japonica gout grain;  gouty arthritis;  matrix metalloproteinase-3;  lipoprotein phospholipase A2
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨忍冬藤痛风颗粒对急性痛风性关节炎模型大鼠的治疗作用及其作用机制。方法:用腺嘌呤和乙胺丁醇灌胃诱导动物高尿酸血症,并在此基础上用尿酸钠制作急性痛风性关节炎模型。观察忍冬藤痛风颗粒对各组大鼠血尿酸水平和肝脏XOD(黄腺呤氧化酶)活性的影响,并检测大鼠关节软组织MMP-3(基质金属蛋白酶-3)及LP-PLA2(腊蛋白磷醋酶A2)含量。结果:忍冬藤痛风颗粒能明显降低模型大鼠血尿酸、肝脏XOD和关节软组织MMP-3及LP-PLA2的表达。结论:忍冬藤痛风颗粒能降低高尿酸血症大鼠血尿酸水平,其机理可能与降低肝脏XOD活性有关; 忍冬藤痛风颗粒能减轻急性痛风性关节炎炎症反应,其作用机制可能与降低血尿酸、减少关节软组织MMP-3及LP-PLA2的表达有关。
Abstract:
Objective: To verify the therapeutic effect of lonicera japonica gout grain in gouty arthritis model rats and explore the mechanism. Methods:The animal hyperuricemia was induced by the use of adenine and ethambutol intragastric, then making acute gouty arthritis model with sodium urate. The effects of lonicera japonica gout grain on blood uric acid level and liver XOD activity was observed and the rat articular soft tissue MMP-3 and LP-PLA2 levels were detected. Results: Lonicera japonica gout grain can significantly reduce blood uric acid,liver XOD and MMP-3, LP-PLA2 expression of joint soft tissue in model rats. Conclusion: Lonicera japonica gout grain can reduce the blood uric acid level in rats with hyperuricemia and the mechanism may be associated with decrease of liver XOD activity. It can also reduce cute gouty arthritis inflammation, which may be associated with decrease of blood uric acid and MMP-3, LP-PLA2 expression of articular soft tissue.

参考文献/References:

[1] 熊湘明,曲竹秋.大鼠高尿酸血症模型建立[J].天津中医学院学报,2001,20(4):28-29.
[2] Coderre TJ,Wall PD.Ankle joint arthritis(AJUA)in rats:an alternative animal model of arthritis to that produced by Freud's adjuvant[J].Pain,1987,28(3):379-393.
[3] 安国尧,张俐.张安桢教授痹症汤的临床应用体会[J].中国中医骨伤科杂志,2012,20(9):65-66.
[4] 周波兰,李郑林,黄东红.深圳平乐骨伤专家李郑林临床用药经验[J].中国中医骨伤科杂志,2013,21(3):66-67.
[5] 邱德华,蔡奇文,张建伟,等.石氏伤科兼邪论及其临床应用[J].中国中医骨伤科杂志,2013,21(1):65-66.
[6] 鲁思爱.忍冬藤的化学成分及其药理应用研究进展[J].临沂大学学报,2012,34(3):132-134.
[7] 刘蕾,刘富,岗杨云,等.忍冬藤多糖抗氧化活性研究[J].中华中医药杂志,2014,29(6):1826-1829.
[8] 蒋毅萍,徐江平.木瓜提取物抗炎镇痛作用的实验研究[J].热带医学杂志,2012,12(11):1335-1337.
[9] 李迎春,徐建华.急性痛风性关节炎的发病机制及研究进展[J].安徽医学,2013,34(1):96-98.
[10] Knapinska A,Fields GB.Chemical biology for understanding matrix metalloproteinase function[J].Chembiochem,2012,13(14):2002-2020.
[11] Merkle M,Ribeiro A,Koppel S.TNF-a enhances TLR3 dependent effects on MMP-9 expression in human mesangial cells[J].Cell Biology International,2012,36(12):1155-1160.
[12] Persson M,Berglund G,Nelson JJ,et al.LP-PLA3 activity and mass are associated with increased incidence of ischemic stroke:a population-based cohort study form Malma,Sweden[J].Atherosclerosis,2008,200(1):191-198.
[13] 李荣华,聂慧,刘友章.中药治疗痛风性关节炎的作用机理研究进展[J].辽宁中医杂志,2010,37(7):1398-1401.
[14] Yang HC,Mosior M,Johnson CA,et al.Group specific assay that distinguish between the four major types of mammalian phospholipase A2[J].Anal Biochem,2002,269(2):278-288.
[15] 俞冰,张剑勇,黄华,等.痛风泰颗粒对急性痛风性关节炎模型大鼠炎症因子的影响[J].中国药房,2013,24(23):2125-2127.

备注/Memo

备注/Memo:
收稿日期:2015-08-01
基金项目:甘肃陇中骨伤学术流派传承项目
西北地区骨伤特色药物疗法重点研究项目
兰州市科技发展计划项目(2013-4-86)
更新日期/Last Update: 2016-02-02