[1]刘楚吟 麻昊宁 董亮 移平 杨峰 唐向盛 谭明生△.维生素A缺乏对新生鼠枕颈部TGF-β1蛋白表达的影响[J].中国中医骨伤科杂志,2015,23(07):5-7.
　LIU Chuyin MA Haoning DONG Liang YI Ping YANG Feng TANG Xiangsheng TAN Mingsheng.The Influence of Vitamin A Deficiency on protein Expression of TGF-β1 in Craniovertebral Junction of Neonatal Mice[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2015,23(07):5-7.
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维生素A缺乏对新生鼠枕颈部TGF-β1蛋白表达的影响
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第23卷
期数:
2015年07期
页码:
5-7
栏目:
实验研究
出版日期:
2015-07-15

文章信息/Info

Title:
The Influence of Vitamin A Deficiency on protein Expression of TGF-β1 in Craniovertebral Junction of Neonatal Mice
文章编号:
1005-0205(2015)07-0005-03
作者:
刘楚吟1; 2 麻昊宁2 董亮2 移平2 杨峰2 唐向盛2 谭明生2△
1.北京中医药大学(北京,100029)
2.中日友好医院
△.通信作者 E-mail:zrtanms@sina.com
Author(s):
LIU Chuyin1; 2 MA Haoning2 DONG Liang2 YI Ping2 YANG Feng2 TANG Xiangsheng2 TAN Mingsheng2
1.Beijing University of Traditional Chinese Medicine, Beijing 100029, China;
2.China-Japan Friendship Hospital, Beijing 100029, China
关键词:
维生素A缺乏;  转化生长因子β1;  枕颈部
Keywords:
vitamin A deficiency;  transforming growth factor β1;  craniovertebral junction
分类号:
R-33
文献标志码:
A
摘要:
目的:通过制作维生素A缺乏模型,检测新生鼠枕颈部骨骼转化生长因子-β1(Transforming Growth Factor-β1,TGF-β1)蛋白表达的情况,探讨枕颈部发育中维生素A对TGF-β1的影响。方法:将健康的雌性C57BL/6 小鼠30只随机分成2组,即正常对照组(Normal Control Group,N组)和维生素A缺乏组(Vitamin A Deficiency Group,VAD组),每组各15只。N组给予AIN-93M配方饲料喂养,VAD组给予维生素A缺乏饲料(根据AIN-93M配方修改)喂养。造模成功后,于妊娠E21 d,取新生鼠枕颈部骨骼,采用免疫组化检测新生胎鼠枕颈部TGF-β1蛋白表达的情况。结果:经过10周的维生素A缺乏饲料喂养,VAD组小鼠出现维生素A缺乏症状,ELISA检测血清维生素A水平明显低于正常对照组(P<0.05)。免疫组化检测显示,VAD组检测新生胎鼠枕颈部TGF-β1蛋白表达水平降低,与N组比较差异有统计学意义(P<0.05)。结论:发现维生素A缺乏导致新生胎鼠枕颈部骨骼中TGF-β1蛋白表达降低,可能与维生素A与TGF-β1信号通路的相互作用有关。
Abstract:
Objective:To observe expression on transforming growth factor β1(TGF-β1)in mice embryo through vitamin A deficiency in pregnant mice model, which aim to investigate the relationship between vitamin A and TGF-β1 in development of the craniovertebral junction. Methods: 30 female C57BL/6 mice were divided into 2 groups, namely normal control group(N)and vitamin A deficiency group(VAD), 15 mice in each group. N group were fed with normal diet, while VAD group were fed with vitamin A deficiency diet. On 21st gestational day after modeling success, craniovertebral junction of neonatal mice were taken for detecting the protein expression TGF-β1 by immunohistochemistry. Results: After 10 weeks of vitamin A deficiency diet, VAD group female mice showed symptoms of vitamin A deficiency, and serum vitamin A level were significantly lower in VAD group than that in N group(P<0.05). And the protein expression levels of TGF-β1 were significantly lower in VAD group than that in N group(P<0.05). Conclusion: In our study, we suggest that vitamin A deficiency could decrease the protein expression level of TGF-β1 in craniovertebral junction of neonatal mice,which might be related to the interaction between vitamin A and TGF-β1 signaling pathway.

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备注/Memo

备注/Memo:
收稿日期:2015-02-13
基金项目:国家自然科学基金(81173423)
更新日期/Last Update: 2015-07-10