[1]何伟,李博宁,黎祥胜,等.健骨方干预大鼠膝骨关节退变的实验研究[J].中国中医骨伤科杂志,2015,23(04):5-7.
　HE Wei,LI Boning,LI Xiangsheng,et al.Experimental Study of Jiangu Decoction Intervention on Knee Joint Degeneration in Rats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2015,23(04):5-7.
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健骨方干预大鼠膝骨关节退变的实验研究
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第23卷
期数:
2015年04期
页码:
5-7
栏目:
实验研究
出版日期:
2015-04-15

文章信息/Info

Title:
Experimental Study of Jiangu Decoction Intervention on Knee Joint Degeneration in Rats
文章编号:
1005-0205(2015)04-0005-03
作者:
何伟1； 李博宁2； 黎祥胜1； 杨功旭1； 彭静2；
1.湖北省中医院(武汉,430074)
2.湖北中医药大学
Author(s):
HE Wei1； LI Boning2； LI Xiangsheng1； YANG Gongxu1； PENG Jing2；
1. Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430074,China
2. Hubei University of Chinese Medicine,Wuhan 430065,China
关键词:
病理;  骨关节炎;  软骨细胞;  健骨方
Keywords:
pathology;  osteoarthritis;  chondrocytes;  Jiangu decoction
分类号:
R-33
文献标志码:
A
摘要:
目的:观察健骨方对大鼠膝骨关节炎(OA)模型关节软骨的组织形态学的影响。方法:选用SD大鼠30只,随机分为正常组,模型组,健骨方组,采用Hulth 法制备大鼠膝骨关节炎模型,以健骨方灌胃为干预治疗手段,观察各组髁关节软骨大体、光镜下的病理变化。结果:正常组股骨髁关节软骨光滑如常; 模型组大鼠膝关节软骨组织出现坏死,失去原有结构,潮线破坏,坏死组织脱落,有大量纤维组织增生,Mankin's 评分与正常组比较差异有统计学意义(P<0.05); 健骨方组软骨表面局部粗糙不平、溃疡形成,移行层有水肿、变形,在坏死区两侧可见软骨基质,染色不一。 结论:健骨方对 Hulth 模型所致的骨关节炎软骨损伤有明显的保护作用。
Abstract:
To observe the influence of Jiangu decoction on knee osteoarthritis(OA)in rats model of articular cartilage histomorphology.Methods:40 SD rats were chosen to make rats model of knee osteoarthritis by Hulth technique,and Jiangu decoction was fed for therapeutic intervention.Pathological change of condylar cartilage was observed in general and in the light microscope.Results:The femoral condyle articular cartilage is smooth as usual in normal group.Knee cartilage tissue necrosis occurred in model group and tide line was damaged.The pathological changes were necrosis and excoriation of cartilage,a large number of fibrotic proliferation.There was significant difference in Mankin's score compared with the that of normal group(P<0.05).In Jiangu decoction group,partial cartilage surface was rough. Ulceration,transitional layer edema and deformation were visible.There were cartilage matrix on both sides of necrotic areas with different staining.Conclusion:Jiangu decoction has obvious protective effect on osteoarthritis cartilage damage caused by Hulth model.

参考文献/References:

[1] SARAF A,MIKOS A G.Gene delivery strategies for cartilage tissue engineering[J].Adv Drug Deliv Revi, 2006, 58(4):592-603.
[2] Das A,Neher JO, Safranek S. Clinical inquiries:Do hyaluronic acid injections relieve OA knee pain[J]. J Fam Pract, 2009, 58(5): 281-283.
[3] 何伟,方苏亭,王胜利,等.松凝分筋手法治疗膝关节骨性关节炎的临床研究[J].中国中医骨伤科杂志,2010,9(2):27-29.
[4] 范全. 松凝分筋手法治疗膝关节骨性关节炎的临床应用[J]. 按摩与导引,2007, 23(6):31-32.
[5] 方苏亭,王胜利,王希,等. 松凝分筋手法治疗膝骨关节病的实验研究[J].中国中医骨伤科杂志,2001,9(2):27-29.
[6] 王胜利,方肇年,李强,等. 李同生教授骨伤科用药特色[J].中国中医骨伤科杂志,1997,5(1):60-61.
[7] HUNTER D J. Imaging insights on the epidemiology and pathophysiology of osteoarthritis[J].Rheum Dis Clin North Am,2009,3:447-463.
[8] 黄际河,张亚峰,王贤波,等.p38在大鼠骨性关节炎模型中的表达[J].江苏医药,2008,34(5):487-489.
[9] GIANNINI E H,RUPERTO N,RAVELLI A,et al.Preliminary definition of improvement in juvenile arthritis[J].arthritis Rheum,1997,40:1202-1209.
[10] 韩清民,刘洪江,梁祖建.补肾方对肾虚型大鼠膝骨关节炎软骨病变基因表达谱的影响[J].中国组织工程研究与临床康复,2008,12(46):9036-9040.
[11] 曾意荣,樊粤光,吴凡,等.膝骨关节炎患者血清基质金属蛋白酶3、肿瘤坏死因子α、白细胞介素1、透明质酸、脂质过氧化物含量与超氧化物歧化酶活性变化与补肾活血中药的干预[J]. 中国组织工程研究与临床康复,2008,12(28):5436-5439.
[12] SANDRA K,LEO A B J,ALISON M B,et al.Different roles of tumour necrosis factor-α and interleukin-1 in murine streptococcal cell wall arthritis[J].Cytokine,1998,10:690-702.
[13] WEI L,SUN J,WANG Z,et al.CD95-induced osteoarthritic chondrocyte apoptosis and necrosis:dependency on p38 mitogen-activated protein kinase[J]. Arthritis Res Ther,2006,8:37-46.
[14] PARGELLIS C,REGAN J.Inhibitors of p38 mitogen-activated protein kinase for the treatment of rheumatoid arthritis[J].Curr Opin Investig Drugs,2003,4:566-571.
[15] WESTRA J C,LIMBURG P.p38 mitogen-activated protein kinase(MAPK)in rheumatoid arthritis[J].Med Chem,2006,6(8):867-874.

备注/Memo

备注/Memo:
收稿日期:2014-07-08
基金项目:李同生名医工作室传承项目
湖北省自然科学基金项目(2012FFC04501)
湖北省卫生厅中医药科技项目(2012Z-B05)
湖北省教育厅中青年人才项目(Q20121609)
湖北省卫生厅青年科技人才项目(QJX2012-27)
武汉市中青年医学骨干人才项目
更新日期/Last Update: 2015-04-01