[1]郑力铭,王达,孙传睿,等.黄精养肌方通过抑制肌间脂肪浸润改善肌少症的作用研究[J].中国中医骨伤科杂志,2026,34(01):107-112.[doi:10.20085/j.cnki.issn1005-0205.260115]
　ZHENG Liming,WANG Da,SUN Chuanrui,et al.Effects of Huangjing Yangji Decoction on Sarcopenia by Inhibiting Intermuscular Fat Infiltration[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2026,34(01):107-112.[doi:10.20085/j.cnki.issn1005-0205.260115]
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黄精养肌方通过抑制肌间脂肪浸润改善肌少症的作用研究()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第34卷
期数:
2026年01期
页码:
107-112
栏目:
肌少症
出版日期:
2026-01-15

文章信息/Info

Title:
Effects of Huangjing Yangji Decoction on Sarcopenia by Inhibiting Intermuscular Fat Infiltration
文章编号:
1005-0205(2026)01-0107-06
作者:
郑力铭1; 王达1; 孙传睿1; 刘治泽1; 2; 彭啸1; 2; 燕锡童1; 李玲慧1; 3△; 魏戌1; 3△
1中国中医科学院望京医院(北京,100102)
2北京中医药大学
3数智中医防治骨与关节退行性疾病北京市重点实验室
Author(s):
ZHENG Liming1; WANG Da1; SUN Chuanrui1; LIU Zhize1; 2; PENG Xiao1; 2; YAN Xitong1; LI Linghui1; 3△; WEI Xu1; 3△
1Wangjing Hospital of China Academy of Chinese Medical Sciences,Beijing 100102,China; 2Beijing University of Chinese Medicine,Beijing 100029,China; 3Beijing Key Laboratory of Numerical Intelligence Chinese Medicine for Prevention and Treatment of Degenerative Diseases of Bone and Joints,Beijing 100102,China.
关键词:
肌少症;  黄精养肌方;  肌间脂肪浸润;  炎症因子
Keywords:
sarcopenia;  Huangjing Yangji decoction;  intermuscular fat infiltration;  inflammatory factors
分类号:
R285.5
DOI:
10.20085/j.cnki.issn1005-0205.260115
文献标志码:
A
摘要:
目的:探讨黄精养肌方通过抑制肌间脂肪浸润改善肌少症小鼠肌肉功能的作用及机制。方法:采用8月龄SAMP8小鼠作为肌少症模型,随机分为模型组、黄精养肌方低、中、高剂量组(4.94,9.88,19.76 g/(kg?d)),并以同龄SAMR1小鼠作为正常对照组。干预8周后,检测小鼠抓力与跑步耐力,称量肌肉湿重并计算肌重/体重比,通过HE染色观察肌纤维形态并测量横截面积,通过油红O染色和Perilipin-1免疫荧光染色评估肌间脂肪浸润,采用ELISA法检测血清炎症因子(TNF-α,IL-6)及血脂(总胆固醇、甘油三酯)水平。结果:与模型组相比,黄精养肌方中、高剂量能显著增加小鼠腓肠肌与股四头肌的肌重/体重比,差异有统计学意义(P<0.05); 提升抓力与跑步耐力,差异有统计学意义(P<0.05); 增大肌纤维横截面积,差异有统计学意义(P<0.05)。组织学结果显示,黄精养肌方可显著减少肌间脂滴沉积,并下调脂滴蛋白Perilipin-1的表达。同时,该方可显著降低血清TNF-α、IL-6 水平(P<0.05),但总胆固醇、甘油三酯水平差异无统计学意义(P>0.05)。结论:黄精养肌方能有效改善肌少症小鼠的肌肉质量、力量及功能,其作用机制可能与抑制肌间脂肪浸润及降低相关炎症水平有关。
Abstract:
Objective:To investigate the mechanism by which Huangjing Yangji decoction ameliorates sarcopenia by inhibiting intermuscular fat infiltration in mice.Methods:Eight-month-old SAMP8 mice were used as the sarcopenia model and randomly divided into the model group and low-dose,medium-dose,and high-dose Huangjing Yangji decoction groups(4.94,9.88,and 19.76 g/(kg?d)),with age-matched SAMR1 mice serving as the normal control group.After 8 weeks of intervention,forelimb grip strength and treadmill running endurance were measured.Muscle wet weight was measured to calculate the muscle-to-body weight ratio.Hematoxylin-eosin(HE)staining was used to observe muscle fiber morphology and measure the cross-sectional area(CSA).Intermuscular lipid deposition was assessed via Oil Red O staining and Perilipin-1 immunofluorescence staining.Serum levels of inflammatory factors(TNF-α,IL-6)and lipids(TC,TG)were detected by ELISA.Results:Compared with the model group,medium and high doses of Huangjing Yangji decoction significantly increased the gastrocnemius and quadriceps muscle-to-body weight ratios(P<0.05),improved grip strength and running endurance(P<0.05),and increased muscle fiber CSA(P<0.05).Histological results showed that Huangjing Yangji decoction significantly reduced intermuscular fat infiltration and downregulated the expression of the lipid droplet coating protein Perilipin-1.Furthermore,Huangjing Yangji decoction significantly decreased serum levels of TNF-α and IL-6(P<0.05),but had no significant effect on TC or TG levels(P>0.05).Conclusion:Huangjing Yangji decoction can effectively improve muscle mass,strength,and function in sarcopenic mice,and its mechanism of action may be related to the inhibition of intermuscular fat infiltration and the reduction of systemic inflammation.

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(收稿日期:2025-11-11)

备注/Memo

备注/Memo:
基金项目:北京市自然科学基金面上项目(7262217)
中国中医科学院卓越青年科技人才培养专项(CI2024D003)
中国中医科学院优秀青年科技人才培养专项(ZZ17-YQ-014)
中国中医科学院研究生科技创新项目(KC2025020)
△通信作者 E-mail:postdoctorli@163.com(李玲慧)
weixu.007@163.com(魏戌)
更新日期/Last Update: 2026-01-15