[1]施蕾,刘德仁,廖太阳,等.膝痹宁Ⅱ方对膝骨关节炎大鼠疼痛的改善作用[J].中国中医骨伤科杂志,2025,33(08):1-6+11.[doi:10.20085/j.cnki.issn1005-0205.250801]
　SHI Lei,LIU Deren,LIAO Taiyang,et al.Improvement of Pain in Rats with Knee Osteoarthritis by Xibining Ⅱ Formula[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2025,33(08):1-6+11.[doi:10.20085/j.cnki.issn1005-0205.250801]
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膝痹宁Ⅱ方对膝骨关节炎大鼠疼痛的改善作用()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第33卷
期数:
2025年08期
页码:
1-6+11
栏目:
实验研究
出版日期:
2025-08-15

文章信息/Info

Title:
Improvement of Pain in Rats with Knee Osteoarthritis by Xibining Ⅱ Formula
文章编号:
1005-0205(2025)08-0001-06
作者:
施蕾1; 刘德仁1; 廖太阳1; 刘江宇1; 吴鹏1; 茆军1△
1南京中医药大学附属医院(江苏省中医院)(南京,210029)
Author(s):
SHI Lei1; LIU Deren1; LIAO Taiyang1; LIU Jiangyu1; WU Peng1; MAO Jun1△
1Affiliated Hospital of Nanjing University of Chinese Medicine(Jiangsu Province Hospital of Chinese Medicine),Nanjing 210029,China.
关键词:
膝骨关节炎;  膝痹宁Ⅱ方;  外周痛敏;  瞬时受体电位通道
Keywords:
knee osteoarthritis;  Xibining Ⅱ formula;  peripheral hyperalgesia;  TRP channels
分类号:
R285.5
DOI:
10.20085/j.cnki.issn1005-0205.250801
文献标志码:
A
摘要:
目的:探讨膝痹宁Ⅱ方对膝骨关节炎(KOA)模型大鼠疼痛的改善作用,并研究其通过瞬时受体电位通道(TRPs)离子通道和轴突导向因子1(Netrin-1)调控外周痛敏的机制。方法:将40只非特定病原体(SPF)级SD大鼠随机分为对照组、模型组、膝痹宁Ⅱ低剂量组、膝痹宁Ⅱ高剂量组及塞来昔布组。用前交叉韧带横断术构建膝骨关节炎大鼠模型,前交叉韧带横断术后第14天开始灌胃干预,给药28 d后提取各组大鼠背根神经节(DRG)组织。在不同时间点测定大鼠外周痛敏阈值; 用免疫荧光法检测各组大鼠背根神经节组织瞬时受体电位香草酸亚型3(TRPV3)、结直肠癌缺失基因(DCC)、蛋白基因产物9.5(PGP9.5)、即时早期基因c-Fos的平均荧光强度; 用ELISA法检测大鼠血清神经生长因子(NGF)、P物质(SP)的含量; 用q-PCR和Western Blot法分析瞬时受体电位香草酸亚型1(TRPV1)、瞬时受体电位锚蛋白1(TRPA1)、Netrin-1、DCC mRNA和蛋白的表达。结果:与对照组相比,模型组大鼠外周痛敏阈值下降,免疫荧光法检测相应组织中TRPV3、DCC、PGP9.5、c-Fos的平均荧光强度增加,大鼠血清中神经生长因子、P物质含量增加,背根神经节组织中TRPV1、TRPA1、Netrin-1、DCC的蛋白和mRNA表达均上升。与模型组相比,膝痹宁Ⅱ方干预后,外周痛敏阈值上升,TRPV3、DCC、PGP9.5、c-Fos的平均荧光强度减弱,血清中神经生长因子、P物质含量减少,TRPV1、TRPA1、Netrin-1、DCC的蛋白和mRNA表达降低,差异均有统计学意义(P<0.05),且膝痹宁Ⅱ方的浓度越高,改善作用越明显。结论:膝痹宁Ⅱ方可以通过降低TRPs离子通道和Netrin-1的表达,调控外周痛敏,从而缓解膝骨关节炎模型大鼠的疼痛。
Abstract:
Objective:To investigate the effects of Xibining Ⅱ(XBN Ⅱ)formula on the improvement of pain in rats with knee osteoarthritis(KOA),and to study the mechanism of ‘peripheral nociceptive sensitisation' through TRPs ion channels and Netrin-1.Methods:40 SPF-grade SD rats were randomly divided into control group,model group,XBN Ⅱ low-dose group,XBN Ⅱ high-dose group,and celecoxib group.Anterior cruciate ligament transection(ACLT)was used to construct the KOA model,and the gavage intervention was started on the day 14 after ACLT,and the dorsal root ganglion(DRG)tissue was extracted from each group 28 d after administration.Peripheral pain sensitivity thresholds were determined in rats at different time points; Immunofluorescence method was used to detect the average fluorescence intensity of TRPV3,DCC,PGP9.5,and c-Fos in DRG tissues of each group; ELISA was used to detect the levels of serum nerve growth factor(NGF)and substance P(SP)in rats; q-PCR and Western Blot techniques were used to analyse the expression of TRPV1,TRPA1,Netrin-1,and DCC mRNA and protein.Results:Compared with the control group,rats in the model group showed a decrease in peripheral pain threshold,an increase in the mean fluorescence intensity of TRPV3,DCC,PGP9.5,and c-Fos in the corresponding tissues detected by immunofluorescence assay,an increase in the levels of NGF and SP in the serum of rats,and a rise in the expression of TRPV1,TRPA1,Netrin-1,and DCC in the DRG tissues in terms of both protein and mRNA.Compared with the model group,after the intervention of XBN Ⅱ formula,the peripheral pain sensitivity threshold increased,the mean fluorescence intensity of TRPV3,DCC,PGP9.5 and c-Fos decreased,the content of NGF and SP in serum decreased,and the protein and mRNA expression of TRPV1,TRPA1,Netrin-1 and DCC decreased,the differences were statistically significant(P<0.05)and the higher the concentration of XBN Ⅱ,the more obvious the improvement effect.Conclusion:XBN Ⅱ formula can reduce the expression of TRPs ion channels and Netrin-1,modulate the ‘peripheral pain sensitivity',and thus alleviate the pain of KOA rats.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金青年项目(82205143)
江苏省中医院优秀青年博士培养专项(2023QB0122)
江苏省中医院第三批高峰学术人才项目(y2021rc20)
江苏省中医院中医膝骨关节炎临床医学创新中心项目(Y2023zx05)
江苏省医学重点学科/实验室建设单位项目(JSDW202252)
江苏省教育厅研究生创新项目(KYCX24_2227)
△通信作者 E-mail:fsyy00606@njucm.edu.cn
更新日期/Last Update: 2025-08-15