[1]梁桂芳,何智军,林知毅,等.山茱萸通过转化生长因子-β通路改善去卵巢大鼠骨质疏松症[J].中国中医骨伤科杂志,2025,33(02):1-7.[doi:10.20085/j.cnki.issn1005-0205.250201]
　LIANG Guifang,HE Zhijun,LIN Zhiyi,et al.Cornus Ameliorates Osteoporosis in Ovariectomized Rats through the TGF-β Signaling Pathway[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2025,33(02):1-7.[doi:10.20085/j.cnki.issn1005-0205.250201]
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山茱萸通过转化生长因子-β通路改善去卵巢大鼠骨质疏松症()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第33卷
期数:
2025年02期
页码:
1-7
栏目:
实验研究
出版日期:
2025-02-15

文章信息/Info

Title:
Cornus Ameliorates Osteoporosis in Ovariectomized Rats through the TGF-β Signaling Pathway
文章编号:
1005-0205(2025)02-0001-07
作者:
梁桂芳1; 何智军1; 林知毅1; 刘锋1△
1广州市番禺区中医院(广州,511400)
Author(s):
LIANG Guifang1; HE Zhijun1; LIN Zhiyi1; LIU Feng1△
1Panyu District Traditional Chinese Medicine Hospital,Guangzhou 511400,China.
关键词:
山茱萸;  绝经后骨质疏松症;  转化生长因子-β信号通路;  Ⅰ型胶原蛋白
Keywords:
Cornus;  postmenopausal osteoporosis;  TGF-β signaling pathway;  type Ⅰ collagen
分类号:
R285.5
DOI:
10.20085/j.cnki.issn1005-0205.250201
文献标志码:
A
摘要:
目的:探讨山茱萸对去卵巢骨质疏松症大鼠骨的保护作用及机制。方法:将50只SD大鼠随机分为假手术组、模型组、雌激素组、山茱萸组和山茱萸+TGF-β抑制剂组,每组10只。假手术组大鼠仅行腹部切开缝合术,其余四组大鼠均切除双侧卵巢建立去卵巢骨质疏松症大鼠模型。山茱萸组大鼠给予山茱萸灌胃,雌激素组大鼠采用炔雌醇灌胃,山茱萸+TGF-β抑制剂组大鼠给予山茱萸灌胃及TGF-β腹腔注射,假手术组及模型组大鼠则予等量生理盐水灌胃。显微CT(Micro CT)检测各组大鼠股骨小梁的微观结构(骨矿物质密度(BMD)、骨矿物质含量(BMC)、骨小梁体积分数(BV/TV)、骨小梁厚度(Tb.Th)、骨表面体积比(BS/BV)和骨小梁分离度(Tb.Sp)),小动物骨密度仪和骨强度检测仪检测股骨骨质情况,Western Blot法检测TGF-β/Smad通路和Ⅰ型胶原蛋白的蛋白含量,酶联免疫吸附测定(ELISA)法检测血清骨标志物(BALP、PⅠNP、OCN和CTX-Ⅰ)含量。结果:山茱萸可显著提高BMD、BMC、BV/TV和Tb.Th,而显著降低BS/BV和Tb.Sp(P<0.01),同时增强骨密度和骨硬度。山茱萸还能下调模型组大鼠血清BALP和CTX-Ⅰ水平,上调PⅠNP和OCN水平(P<0.01)。此外,进一步的机制探究显示,山茱萸可调节模型组大鼠股骨中的TGF-β/Smad信号通路和胶原蛋白水平。结论:山茱萸可改善模型组大鼠的股骨骨质,减轻骨质疏松严重程度,其机制可能与调节TGF-β/Smad通路和Ⅰ型胶原蛋白有关。
Abstract:
Objective:To investigate the protective effect and mechanism of Cornus on bone of ovariectomized osteoporosis rats.Methods:50 SD rats were randomly divided into sham group,model group,estrogen group,Cornus group,and Cornus+TGF-β inhibitor group,with 10 rats in each group.The rats in the sham operation group only underwent abdominal incision and suture,and the other four groups underwent bilateral ovariectomy to establish ovariectomized osteoporosis model.Rats in the Cornus group were given Cornus by gavage,while rats in the estrogen group were given ethinyl estradiol by gavage.TGF-β inhibitor was injected intraperitoneally in the TGF-β inhibitor group,while rats in the sham groups were given the same amount of physiological saline by gavage.Micro CT was used to detect the microstructure of femoral trabeculae in each group(bone mineral density(BMD),bone mineral content(BMC),bone trabecular volume fraction(BV/TV),trabecular thickness(Tb.Th),bone surface-to-volume ratio(BS/BV),and trabecular separation(Tb.Sp)).The femoral bone quality was detected by small animal bone densitometer and bone strength tester.Western blot was used to detect the protein levels of TGF-β/Smad pathway and type Ⅰ collagen,and enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of serum bone markers(BALP,PⅠNP,OCN,and CTX-Ⅰ).Results:The results showed that Cornus could significantly increase BMD,BMC,BV/TV,and Tb.Th,while significantly reducing BS/BV and Tb.Sp(P<0.01),and enhancing bone density and bone stiffness.Cornus could also downregulate the serum levels of BALP and CTX-Ⅰ in model rats and upregulate the levels of PⅠNP and OCN(P<0.01).Furthermore,the mechanism exploration showed that Cornus could regulate the TGF-β/Smad signaling pathway and collagen levels in the femoral bone of model rats.Conclusion:The results suggested that Cornus could improve femoral bone quality and reduce the severity of osteoporosis in model rats,which might be related to the regulation of TGF-β/Smad pathway and type Ⅰ collagen.

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备注/Memo

备注/Memo:
基金项目:广东省中医药局科研项目(20241227)
△通信作者 E-mail:15813358903@163.com
更新日期/Last Update: 2025-02-15