[1]刘哲△,阿良,张勇,等.盘龙七片对小鼠骨折愈合过程中血管生成的影响[J].中国中医骨伤科杂志,2023,31(01):13-18.
　LIU Zhe,A Liang,ZHANG Yong,et al.Efficacy of Panlongqi Tablet on Angiogenesis during Bone Fracture Healing in Mice[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2023,31(01):13-18.
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盘龙七片对小鼠骨折愈合过程中血管生成的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第31卷
期数:
2023年01期
页码:
13-18
栏目:
实验研究
出版日期:
2023-01-15

文章信息/Info

Title:
Efficacy of Panlongqi Tablet on Angiogenesis during Bone Fracture Healing in Mice
文章编号:
1005-0205(2023)01-0013-06
作者:
刘哲1△; 阿良1; 张勇1; 季英楠1; 孟建国2
1沈阳医学院附属中心医院骨二科(沈阳110024) 2陕西盘龙药业集团股份有限公司
Author(s):
LIU Zhe1△; A Liang1; ZHANG Yong1; JI Yingnan1; MENG Jianguo2
1Second Department of Orthopaedics,Central Hospital Affiliated to Shenyang Medical College,Shenyang 110024,China; 2Shaanxi Panlong Pharmaceutical Group Co.Ltd.,Xi'an 710065,China.
关键词:
关键词]盘龙七片;  骨折愈合;  血管生成;  人脐静脉内皮血管细胞
Keywords:
Panlongqi tablet;  fracture healing;  angiogenesis;  human umbilical vein endothelial cell(HUVEC)
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨盘龙七片对小鼠骨折愈合中血管生成的影响。方法:采用45只C57BL/6小鼠制备髓内针固定小鼠闭合性股骨骨折模型,随机分为3组(对照组、低剂量组和高剂量组)。于造模第2天开始灌胃给药盘龙七片,对照组给予等量生理盐水灌胃,高剂量组和低剂量组分别按照盘龙七片高剂量(1.28 g/kg)及低剂量(0.25 g/kg)灌胃给药,2次/d,连续4周。在给药3周后,采用颈椎脱臼方法处死动物,收集损伤股骨制备骨组织切片,行苏木精-伊红(HE)染色检测造模骨折愈合情况; 在给药后第1,2,3,4周后取受损骨组织,收集总蛋白,利用Western Blot实验检测其中促血管生成因子血管生成素-1(Ang-1)、碱性成纤维细胞生长因子(bFGF)、血管内皮生长因子(VEGF)和血小板-内皮细胞黏附分子(CD31)的蛋白表达量。利用盘龙七片处理人脐静脉内皮血管细胞(HUVEC),行体外小管形成实验、Transwell侵袭实验检测盘龙七片对小管形成及HUVEC侵袭能力的影响。结果:HE染色显示高剂量组盘龙七片处理3周后,骨痂区域充满大量的类骨质,骨折愈合情况显著高于低剂量组及对照组。Western Blot结果表明,高剂量组和低剂量组盘龙七片均可不同程度促进Ang-1、bFGF、VEGF和CD31的蛋白表达,且高剂量组中Ang-1、bFGF、VEGF和CD31蛋白表达量显著高于低剂量组,差异有统计学意义(P<0.05)。高剂量组和低剂量组盘龙七片均可促进HUVEC小管形成和提高其侵袭能力,且高剂量组盘龙七片可更有效地加速血管形成和细胞侵袭,差异有统计学意义(P<0.05)。结论:盘龙七片可促进骨折部位血管生成因子的表达,加速HUVEC小管形成和提高其侵袭能力,且高剂量的促进作用更加明显。
Abstract:
Objective:To explore the efficacy of Panlongqi tablet on angiogenesis during fracture healing in mice.Methods:45 SD mice were used to establish a femoral fracture model and were randomly divided into three groups:control group,low-dose group and high-dose group.Panlongqi tablets were administered by gavage on the second day of surgery.Control group was given intragastric saline.The high-dose group and the low-dose group were administered intragastrically according to the high dose(1.28 g/kg body weight)and low dose(0.25 g/kg body weight)of Panlongqi tablets.All groups were treated twice a day for 4 consecutive weeks.After 3 weeks of administration,the animals were sacrificed by cervical dislocation,and the fractured femurs were collected to prepare bone tissue sections.Hematoxylin-eosin(HE)staining was performed to detect the healing of the model fracture.Fractured bone tissue was collected after 1,2,3,and 4 weeks of administration.Total protein was collected.Western Blot experiments were used to detect the protein expressions of angiogenesis factors Angiopoietin-1(Ang-1),basic fibroblast growth factor(bFGF),vascular endothelial growth factor(VEGF)and platelet-endothelial cell adhesion molecule(CD31)in callus.The human umbilical vein endothelial cell(HUVEC)was treated by Panlongqi tablet,and the efficacy of Panlongqi tablet on tubule formation and invasion ability of HUVEC detected by in vitro tubule formation test and Transwell invasion test.Results:HE staining showed that callus area filled with plenty of osteoid bone in high-dose group 3 weeks after modeling,and the fracture healing in the high-dose group was better than those in the low-dose group and control group.The results of Western Blot showed that Panlongqi tablet in high-dose group and low-dose group can promote the protein expressions of Ang-1,bFGF,VEGF and CD31,and the expression levels of Ang-1,bFGF,VEGF and CD31 in the callus of high-dose group were relatively higher than that in low-dose group.Panlongqi tablets in high-dose group and low-dose group can promote the tubule formation and migration of HUVEC,and Panlongqi tablets in high-dose group can more effectively accelerate blood vessel remodeling and cell migration.Conclusion:Panlongqi tablets can promote the expression of angiogenesis factors at the fracture site,accelerate the tubule formation and migration ability of HUVEC,and the acceleration of high-dose is more obvious.

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备注/Memo

备注/Memo:
基金项目:盘龙七片上市再评价研究(2020ZDLSF03-10)
△通信作者 E-mail:zheliu_drl@163.com
更新日期/Last Update: 2023-01-10