[1]刘亚梅,王斌,罗毅文,等.补肾活血法调控大鼠骨髓间充质干细胞增殖活性的实验研究[J].中国中医骨伤科杂志,2015,23(03):1-4.
　LIU Yamei,WANG Bin,LUO Yiwen,et al.Study of BuShenHuoXue Decoction Medicated the Proliferation ofRat Bone Marrow Mesenchymal Stem Cells[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2015,23(03):1-4.
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补肾活血法调控大鼠骨髓间充质干细胞增殖活性的实验研究
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第23卷
期数:
2015年03期
页码:
1-4
栏目:
实验研究
出版日期:
2015-03-15

文章信息/Info

Title:
Study of BuShenHuoXue Decoction Medicated the Proliferation ofRat Bone Marrow Mesenchymal Stem Cells
文章编号:
1005-0205(2015)03-0001-04
作者:
刘亚梅1；王斌2；罗毅文2；陈东风1；胡年宏2；黄永铨2；钱伟宏3；张迅超2；李汪洋2；徐志明4△；候秋科1
1广州中医药大学基础医学院(广州,510006)
2广州中医药大学附属骨伤科医院
3浙江省桐乡市中医院
4广东省江门市五邑中医院
Author(s):
LIU Yamei1；WANG Bin2；LUO Yiwen2；CHEN Dongfeng2；HU Nianhong1；HUANG Yongquan2；QIAN Weihong3；ZHANG Xunchao2；LI Wangyang2；XU Zhiming4；HOU Qiuke1
1School of Basic Medical Sciences, Guang Zhou University of Traditional Chinese Medicine, Guang Zhou 510405, China
2Traumatology Hospital, Affiliated to GuangZhou University of TCM, Guang Zhou 510240, China
3TongXiang Hospital of TCM, TongXiang, 314500, China
4JiangMen Wuyi Hospital of TCM, JiangMen 529000, China
关键词:
补肾活血法;  骨髓间充质干细胞;  增殖;  含药血清
Keywords:
strengthening kidney and activating circulation;  bone marrow mesenchymal stem cells;  proliferation;  drug serum
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨补肾活血汤及其拆方含药血清调控大鼠骨髓间充质干细胞(BMSCs)增殖活性的作用。方法:体外骨髓贴壁法分离培养大鼠BMSCs,取第3代细胞,免疫荧光法鉴定细胞表面抗原,成骨及成脂诱导证实其多向分化潜能; 实验分为补肾活血汤全方组、补肾组、活血组及空白对照组,运用补肾活血汤及其拆方含药血清干预第3代 BMSCs 48 h,并用四甲基偶氮唑盐比色法(MTT)观察细胞增殖活性,流式细胞仪检测细胞周期。结果:MTT结果显示:体积分数5%及10%补肾活血汤全方含药血清均有促进大鼠BMSCs增殖的作用; 流式细胞仪检测细胞周期显示:运用补肾活血汤含药血清干预后,处于增值分裂期(S+G2+M期)的BMSCs百分率增多,与其他3组比较差异有统计学意义(P<0.05)。结论:补肾活血汤全方含药血清有促进大鼠骨髓间充质干细胞增殖的作用。
Abstract:
Objective: To investigate the effect of drug serum of BuShenHuoXue decoction and its separated prescription medicated proliferation of rat bone marrow mesenchymal stem cells(BMSCs). Methods: Rat BMSCs were cultured by adherence method in vitro. The third generation of cells was collected. Cell surface antigen was identified by immunofluorescence assay. Osteogenesis and adipogenesis were induced to confirm its multi-directional differentiation potential. The study includes BuShenHuoXue decoction group, strengthening kidney group, activating circulation group and control group. The third generation of BMSCs was treated with drug serum of BuShenHuoXue decoction and its separated prescription by 48 hours. Cell proliferation was determined by MTT assay. Cell cycle was examined by flow-cytometric analysis. Results: MTT shows 5% and 10% drug serum of BuShenHuoXue decoction have the ability to stimulate bone marrow mesenchymal stem cell proliferation. Flow-cytometric analysis shows percentage of BMSCs in proliferative stage(S+G2+M stages)was increased when treated with drug serum of BuShenHuoXue decoction, and there were statistical differences among the groups. Conclusion: Drug serum of BuShenHuoXue decoction can stimulate rat bone marrow mesenchymal stem cell proliferation.

参考文献/References:

[1] 王斌,刘亚梅,罗毅文,等.SD大鼠皮质骨来源间充质干细胞的培养与鉴定[J].广州中医药大学学报,2014,3(31):452-454.
[2] DOMINICI M,LE BLANC K,MUELLER I,et al.Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement[J]. Cytotherapy,2006,8(4):315-317.
[3] ZOU D,ZHANG Z,YE D X,et al.Repair of Critical-Sized Rat Calvarial Defects Using Genetically Engineered Bone Marrow-Derived Mesenchymal Stem Cells Overexpressing Hypoxia-Inducible Factor-1α[J].Stem Cells,2011,29(9):1380-1390.
[4] PITTENGER M F,MACKAY A M,BECK S C,et al. Multilineage potential of adult human mesenchymal stem cells[J].science,1999,284(5411):143-147.
[5] LWAMA H,AMAGAYA S,OGILARA Y.Effect of shosaikoto,a Japanese and Chinese traditional herbal medicinal mixture,on the mitogenic activity of lipopolys -accharide:a new pharmacological testing method[J].J Ethnopharmacol,1987,21(1):45-53.
[6] 李春根,宋广明,康晓乐,等.补肾活血汤结合组织工程软骨修复兔膝关节软骨缺损[J].医学研究杂志,2011,40(9):62-65.
[7] 苏继承,刘明武.补肾活血汤治疗骨折后期断端骨质疏松46例[J].中国中医骨伤科杂志,1998,6(2):37-38.
[8] 王书湘.中医辨证结合手术治疗骨不连46例[J].河南中医,2011,31(8):903-904.

备注/Memo

备注/Memo:
收稿日期:2014-08-10
基金项目:国家自然科学基金项目(编号:NO.81273783)
△通讯作者 E-mail:bamei2007@126.com
更新日期/Last Update: 2015-03-01