[1]何晓丹,鲍荣华,丁芳平,等.张氏肌痹洗方熏洗治疗膝骨关节炎的机制研究[J].中国中医骨伤科杂志,2024,32(06):30-34.[doi:10.20085/j.cnki.issn1005-0205.240606]
　HE Xiaodan,BAO Ronghua,DING Fangping,et al.Study on Mechanism of Fumigation with Zhang's Muscular Arthralgia Washing Prescription in Treating Knee Osteoarthritis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2024,32(06):30-34.[doi:10.20085/j.cnki.issn1005-0205.240606]
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张氏肌痹洗方熏洗治疗膝骨关节炎的机制研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第32卷
期数:
2024年06期
页码:
30-34
栏目:
实验研究
出版日期:
2024-06-15

文章信息/Info

Title:
Study on Mechanism of Fumigation with Zhang's Muscular Arthralgia Washing Prescription in Treating Knee Osteoarthritis
文章编号:
1005-0205(2024)06-0030-05
作者:
何晓丹1; 鲍荣华1; 丁芳平1; 杨信波1
1杭州市富阳中医骨伤医院(杭州,311400)
Author(s):
HE Xiaodan1; BAO Ronghua1; DING Fangping1; YANG Xingbo1
1Hangzhou Fuyang Bone Injury Hospital of Traditional Chinese Medicine,Hangzhou 311400,China.
关键词:
机械敏感性离子通道;  张氏肌痹洗方;  熏洗;  机制
Keywords:
mechanically sensitive ion channels;  Zhang's muscular arthralgia washing prescription;  fumigation washing;  mechanism
分类号:
R684.3
DOI:
10.20085/j.cnki.issn1005-0205.240606
文献标志码:
A
摘要:
目的:基于Piezo机械敏感性离子通道探究张氏肌痹洗方熏洗治疗膝骨关节炎(KOA)的机制。方法:选取成年雌性SD大鼠,随机分为空白组、模型组、模型+阻断剂组、模型+中药熏洗组和模型+中药熏洗+阻断剂组,每组各10只。其中模型组的大鼠采用下肢石膏固定法构建膝骨关节炎模型; 模型+阻断剂组于造模后第1天开始,分别在右侧足底局部注射GsMTx-4,100 μL/次,1次/d,连续7 d; 模型+中药熏洗组和模型+中药熏洗+阻断剂组在造模后第4天分别介入中药熏洗,中药熏洗20 min,1次/d,连续干预21 d。之后各组大鼠处死,截取左后肢膝关节滑膜组织作为标本,采用ELISA法测定关节液中IL-1β、IL-6和TNF-α的表达量; Western Blot法检测大鼠左膝关节滑膜组织中Piezo两型及ERK1/2的蛋白表达量; RT-PCR 法检测Piezo两型及ERK1/2的mRNA相对表达量。结果:与空白组比较,模型组大鼠膝关节滑膜组织中Piezo两型及ERK1/2的表达量均明显升高,差异有统计学意义(P<0.05); 与模型组比较,模型+阻断剂组大鼠左膝关节滑膜组织中Piezo两型及ERK1/2的含量均明显降低,差异有统计学意义(P<0.05)。与空白组比较,模型组大鼠膝关节腔内关节液中IL-1β、IL-6和TNF-α表达水平均明显升高,差异有统计学意义(P<0.05); 与模型组比较,模型+中药熏洗组和模型+中药熏洗+阻断剂组大鼠膝关节腔内关节液中IL-1β、IL-6和TNF-α表达水平均明显降低,差异有统计学意义(P<0.05)。与空白组比较,模型组大鼠左膝关节滑膜组织中Piezo两型、ERK1/2表达水平及mRNA表达量均明显升高,差异有统计学意义(P<0.05); 与模型组比较,模型+中药熏洗组和模型+中药熏洗+阻断剂组大鼠左膝关节滑膜组织中Piezo两型、ERK1/2表达水平及mRNA表达量均明显降低,差异有统计学意义(P<0.05)。结论:张氏肌痹洗方熏洗能够通过调控Piezo机械敏感性离子通道改善炎症因子水平,进而缓解关节的退变。
Abstract:
Objective:To explore the mechanism of fumigation with Zhang's muscular arthralgia washing prescription in treating knee osteoarthritis(KOA)based on Piezo mechanical sensitivity ion channel.Methods:Adult female SD rats were selected and randomly divided into blank group,model group,model+blocker group,model+TCM washing group,model+TCM washing+blocker group,respectively,with 10 animals in each group.The rats in the model group constructed KOA model by lower limb plaster.The model+blocker group injected GsMTx-4 on the right foot on the first day,100 μL,once a day for 7 consecutive days.The model+TCM washing group and model+TCM washing+blocker group were respectively intervened with TCM washing on the 4th day after modeling,for 20 min each time,once a day,for 21 consecutive days.Rats were killed later and synovial tissue of left hindlimb knee was taken as specimen.IL-1β,IL-6,and TNF-α in joint fluid were measured by ELISA.The protein expression of Piezo and ERK1/2 in synovial tissue of left knee was measured by Western Blot.The relative mRNA expression of Piezo and ERK1/2 was measured by RT-PCR.Results:The expression levels of Piezo two types and ERK1/2 in the knee synovial tissue of the model group rats were significantly increased compared with the blank group(P<0.05).The content of Piezo two types and ERK1/2 in the synovial tissue of the left knee joint of rats in the model+blocker group was significantly reduced compared with the model group(P<0.05).The expression levels of IL-1β and IL-6 and TNF-α in the joint fluid of the knee joint cavity of the model group rats were significantly increased compared with the blank group(P<0.05).The expression levels of IL-1β and IL-6 and TNF-α in the joint fluid of rats in the knee joint cavity were significantly reduced in both model+TCM washing group and model+TCM washing+blocker group compared with the model group(P<0.05).The expression levels and mRNA levels of Piezo two types and ERK1/2 in the synovial tissue of the left knee joint of the model group rats were significantly increased compared with the blank group(P<0.05).The expression levels and mRNA levels of Piezo two types and ERK1/2 in the synovial tissue of the left knee joint of rats in both model+TCM washing group and model+TCM washing+blocker group were significantly reduced compared with the model group(P<0.05).Conclusion:Zhang's muscular arthralgia washing prescription can improve the level of inflammatory factors by regulating Piezo mechanical sensitivity ion channel,and then alleviate the degeneration of joints.

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更新日期/Last Update: 2024-06-15