[1]严铮,徐妍,刘应泉,等.外泌体影响关节软骨再生修复及软骨下骨骨代谢平衡的研究进展[J].中国中医骨伤科杂志,2024,32(05):92-96.[doi:10.20085/j.cnki.issn1005-0205.240518]
 YAN Zheng,XU Yan,LIU Yingquan,et al.The Mechanism and Application Progress of Exosomes in the Treatment of Osteoarthritis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2024,32(05):92-96.[doi:10.20085/j.cnki.issn1005-0205.240518]
点击复制

外泌体影响关节软骨再生修复及软骨下骨骨代谢平衡的研究进展()
分享到:

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第32卷
期数:
2024年05期
页码:
92-96
栏目:
文献综述
出版日期:
2024-05-05

文章信息/Info

Title:
The Mechanism and Application Progress of Exosomes in the Treatment of Osteoarthritis
文章编号:
1005-0205(2024)05-0092-05
作者:
严铮1徐妍2刘应泉1吴连国3△
1浙江中医药大学第二临床医学院(杭州,310053)
2浙江中医药大学护理学院
3浙江中医药大学附属第二医院
Author(s):
YAN Zheng1XU Yan2LIU Yingquan1WU Lianguo3△
1Second Clinical Medical College of Zhejiang Chinese Medical University,Hangzhou 310053,China; 2School of Nursing,Zhejiang Chinese Medical University,Hangzhou 310053,China; 3Second Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310005,China.
关键词:
外泌体 骨关节炎 关节软骨 软骨细胞 软骨下骨
Keywords:
exosome osteoarthritis articular cartilage chondrocyte subchondral bone
分类号:
R684.3
DOI:
10.20085/j.cnki.issn1005-0205.240518
文献标志码:
A
摘要:
外泌体作为一种细胞释放到胞外的小型囊泡,是细胞间通信与物质呈递的天然运输载体,其内含诸多来自分泌细胞的生物活性物质,包括各类核糖核酸、蛋白质、脂质、转录因子以及细胞因子受体等。随着对外泌体及其内含物研究的不断深入,发现外泌体可以通过影响软骨细胞、破骨细胞和成骨细胞的生存和功能,在骨关节炎软骨及软骨下骨的修复与重塑中发挥重要作用,有巨大的治疗潜力。本文主要从外泌体调控软骨细胞的增殖、分化、代谢、程序性细胞死亡,影响软骨下骨成骨细胞和破骨细胞的功能等方面论述其在关节软骨再生修复以及软骨下骨骨代谢平衡中的作用机制。
Abstract:
Exosomes as small vesicles released by cells into the extracellular space,are natural transport carriers for intercellular communication and material presentation.It contains many biologically active substances from secretory cells,including various types of ribonucleic acids,proteins,lipids,transcription factors,and cytokine receptors.With the deepening of the research on exosomes and their inclusions,it is found that exosomes can regulate the microenvironment of articular cavity by affecting the survival and function of chondrocytes,osteoclasts and osteoblasts,and play an important role in the repair and remodeling of osteoarthritis cartilage and subchondral bone,showing great therapeutic potential.This article mainly discusses the mechanism of exosomes in the regeneration and repair of articular cartilage and the metabolic balance of subchondral bone from the aspects of regulating the proliferation,differentiation,metabolism,programmed cell death of chondrocytes,affecting the function of osteoblasts and osteoclasts in subchondral bone.

参考文献/References:

[1] HE L,HE T,XING J,et al.Bone marrow mesenchymal stem cell-derived exosomes protect cartilage damage and relieve knee osteoarthritis pain in a rat model of osteoarthritis[J].Stem Cell Research & Therapy,2020,11(1):276.
[2] MEN Y,YELICK J,JIN S,et al.Exosome reporter mice reveal the involvement of exosomes in mediating neuron to astroglia communication in the CNS[J].Nat Commun,2019,10(1):4136.
[3] LIU W,LI L,RONG Y,et al.Hypoxic mesenchymal stem cell-derived exosomes promote bone fracture healing by the transfer of miR-126[J].Acta Biomater,2020,103:196-212.
[4] SENGUPTA V,SENGUPTA S,LAZO A,et al.Exosomes derived from bone marrow mesenchymal stem cells as treatment for severe COVID-19[J].Stem Cells Dev,2020,29(12):747-754.
[5] WANG D,MING X,XU J,et al.Circ_0009910 shuttled by exosomes regulates proliferation,cell cycle and apoptosis of acute myeloid leukemia cells by regulating miR-5195-3p/GRB10 axis[J].Hematol Oncol,2021,39(3):390-400.
[6] KALLURI R,LEBLEU V S.The biology,function,and biomedical applications of exosomes[J].Science,2020,367(6478):eaau6977.
[7] ZHOU H,SHEN X,YAN C,et al.Extracellular vesicles derived from human umbilical cord mesenchymal stem cells alleviate osteoarthritis of the knee in mice model by interacting with METTL3 to reduce m6A of NLRP3 in macrophage[J].Stem Cell Research & Therapy,2022,13(1):322.
[8] HE X,WANG Y,LIU Z,et al.Osteoporosis treatment using stem cell-derived exosomes:a systematic review and meta-analysis of preclinical studies[J].Stem Cell Res Ther,2023,14(1):72.
[9] SHEN K,DUAN A,CHENG J,et al.Exosomes derived from hypoxia preconditioned mesenchymal stem cells laden in a silk hydrogel promote cartilage regeneration via the miR-205-5p/PTEN/AKT pathway[J].Acta Biomater,2022,143:173-188.
[10] LIAO Q,LI B J,LI Y,et al.Low-intensity pulsed ultrasound promotes osteoarthritic cartilage regeneration by BMSC-derived exosomes via modulating the NF-κB signaling pathway[J].Int Immunopharmacol,2021,97:107824.
[11] LI N,CHEN Z,FENG W,et al.Triptolide improves chondrocyte proliferation and secretion via down-regulation of miR-221 in synovial cell exosomes[J].Phytomedicine,2022,107:154479.
[12] 廖太阳,李晓辰,张力,等.基于柔肝养筋理论研究肝外泌体减少膝骨关节炎软骨凋亡及木瓜的疗效机制[J].中华中医药杂志,2022,37(10):6031-6036.
[13] XU H,XU B.BMSC-derived exosomes ameliorate osteoarthritis by inhibiting pyroptosis of cartilage via delivering miR-326 targeting HDAC3 and STAT1/NF-κB p65 to chondrocytes[J].Mediators Inflamm,2021:9972805.
[14] WEN C,LIN L,ZOU R,et al.Mesenchymal stem cell-derived exosome mediated long non-coding RNA KLF3-AS1 represses autophagy and apoptosis of chondrocytes in osteoarthritis[J].Cell Cycle,2022,21(3):289-303.
[15] JIANG K,JIANG T,CHEN Y,et al.Mesenchymal stem cell-derived exosomes modulate chondrocyte glutamine metabolism to alleviate osteoarthritis progression[J].Mediators Inflamm,2021:2979124.
[16] MENG F,LI Z,ZHANG Z,et al.MicroRNA-193b-3p regulates chondrogenesis and chondrocyte metabolism by targeting HDAC3[J].Theranostics,2018,8(10):2862-2883.
[17] THOMAS B L,ELDRIDGE S E,NOSRATI B,et al.WNT3A-loaded exosomes enable cartilage repair[J].J Extracell Vesicles,2021,10(7):e12088.
[18] SHAO L T,LUO L,QIU J H,et al.PTH(1-34)enhances the therapeutic effect of bone marrow mesenchymal stem cell-derived exosomes by inhibiting proinflammatory cytokines expression on OA chondrocyte repair in vitro[J].Arthritis Res Ther,2022,24(1):96.
[19] SALHOTRA A,SHAH H N,LEVI B,et al.Mechanisms of bone development and repair[J].Nat Rev Mol Cell Biol,2020,21(11):696-711.
[20] JIN Y,XU M,ZHU H,et al.Therapeutic effects of bone marrow mesenchymal stem cells-derived exosomes on osteoarthritis[J].J Cell Mol Med,2021,25(19):9281-9294.
[21] WANG R,XU B.TGFβ1-modified MSC-derived exosome attenuates osteoarthritis by inhibiting PDGF-BB secretion and H-type vessel activity in the subchondral bone[J].Acta Histochem,2022,124(7):151933.
[22] FU Y,CUI S,ZHOU Y,et al.Dental pulp stem cell-derived exosomes alleviate mice knee osteoarthritis by inhibiting TRPV4-mediated osteoclast activation[J].Int J Mol Sci,2023,24(5):4926.
[23] DUAN L,LIANG Y,XU X,et al.Noncoding RNAs in subchondral bone osteoclast function and their therapeutic potential for osteoarthritis[J].Arthritis Res Ther,2020,22(1):279.
[24] LIU Y,ZENG Y,SI H B,et al.Exosomes derived from human urine-derived stem cells overexpressing mir-140-5p alleviate knee osteoarthritis through downregulation of vegfa in a rat model[J].Am J Sports Med,2022,50(4):1088-1105.
[25] HUANG Y,ZHANG X,ZHAN J,et al.Bone marrow mesenchymal stem cell-derived exosomal miR-206 promotes osteoblast proliferation and differentiation in osteoarthritis by reducing ELF3[J].J Cell Mol Med,2021,25(16):7734-7745.
[26] LI B,DING T,CHEN H,et al.CircStrn3 targeting microRNA-9-5p is involved in the regulation of cartilage degeneration and subchondral bone remodelling in osteoarthritis[J].Bone & Joint Research,2023,12(1):33-45.
[27] WU C,ZHU Q,YAO Y,et al.Exosome miR-23a-3p from osteoblast alleviates spinal cord ischemia/reperfusion injury by down-regulating KLF3-activated CCNL2 transcription[J].Dev Neurosci,2022,44(3):121-130.
[28] MA S,LI S,ZHANG Y,et al.BMSC-derived exosomal circhipk3 promotes osteogenic differentiation of MC3T3-E1 cells via mitophagy[J].Int J Mol Sci,2023,24(3):231-242.
[29] WU X,CRAWFORD R,XIAO Y,et al.Osteoarthritic subchondral bone release exosomes that promote cartilage degeneration[J].Cells,2021,10(2):1121-1130.
[30] WANG C,WANG M,XU T,et al.Engineering bioactive self-healing antibacterial exosomes hydrogel for promoting chronic diabetic wound healing and complete skin regeneration[J].Theranostics,2019,9(1):65-76.

备注/Memo

备注/Memo:
基金项目:浙江省中医药现代化专项项目(2021ZX009)
浙江中医药大学科研项目(2023JKZKTS41)
通信作者 E-mail:zcwu@vip.163.com
更新日期/Last Update: 2024-05-15