[1]周化腾,王洪顺,杜伟斌,等.诱导多能干细胞来源表皮干细胞与人脱细胞羊膜组织相容性研究[J].中国中医骨伤科杂志,2024,32(05):14-17.[doi:10.20085/j.cnki.issn1005-0205.240503]
　ZHOU Huateng,WANG Hongsun,DU Weibin,et al.Study on the Histocompatibility between iPSCs Derived Epidermal Stem Cells and Acellular Amniotic Membrane[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2024,32(05):14-17.[doi:10.20085/j.cnki.issn1005-0205.240503]
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诱导多能干细胞来源表皮干细胞与人脱细胞羊膜组织相容性研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第32卷
期数:
2024年05期
页码:
14-17
栏目:
实验研究
出版日期:
2024-05-05

文章信息/Info

Title:
Study on the Histocompatibility between iPSCs Derived Epidermal Stem Cells and Acellular Amniotic Membrane
文章编号:
1005-0205(2024)05-0014-04
作者:
周化腾1; 王洪顺1; 杜伟斌1; 翟晓军1
1浙江中医药大学附属江南医院(杭州市萧山区中医院)(杭州,311200)
Author(s):
ZHOU Huateng1; WANG Hongsun1; DU Weibin1; ZHAI Xiaojun1
1Jiangnan Hospital Affiliated to Zhejiang Chinese Medical University,(Hangzhou Xiaoshan Hospital of Traditional Chinese Medicine)Hangzhou 311200,China.
关键词:
诱导多能干细胞;  表皮干细胞;  羊膜;  皮肤组织工程
Keywords:
induced pluripotent stem cells;  epidermal stem cells;  amniotic membrane;  skin tissue engineering
分类号:
R-33
DOI:
10.20085/j.cnki.issn1005-0205.240503
文献标志码:
A
摘要:
目的:研究诱导多能干细胞(iPSCs)来源的表皮干细胞与人脱细胞羊膜的组织相容性,分析其构建皮肤替代物的可行性。方法:通过调控类风湿关节炎(RA)、骨形态发生蛋白4(BMP4)、表皮细胞生长因子(EGF)信号通路,将诱导多能干细胞定向分化为表皮干细胞,随后将该细胞种植于人脱细胞羊膜,以苏木精-伊红(HE)染色、细胞计数试剂(CCK-8试剂)及扫描电子显微镜检测诱导多能干细胞来源表皮干细胞与人脱细胞羊膜的生物相容性。结果:诱导多能干细胞来源的细胞具有类似表皮干细胞形态、表型和基因表达,该细胞培养于人脱细胞羊膜,呈紧密薄片状生长,且增殖力与对照组有显著差异。结论:诱导多能干细胞来源表皮干细胞与人脱细胞羊膜具有较好的生物相容性,是具有潜力的种子细胞。
Abstract:
Objective:To investigate the histocompatibility of epidermal stem cells derived from induced pluripotent stem cells(iPSCs)with human acellular amniotic membrane,and to explore the feasibility of using these cells to construct skin substitutes.Methods:By regulating the rheumatoid arthritis(RA),bone morphogenetic protein 4(BMP4),and epidermal growth factor(EGF)signaling pathways,iPSCs were directed to differentiate into epidermal stem cells.Subsequently,the cells were implanted into the human acellular amniotic membrane,and the biocompatibility of iPSCs derived epidermal stem cells with the human acellular amniotic membrane was detected using hematoxylin-eosin(HE)staining,Cell-Counting-Kit-8(CCK-8),and scanning electron microscopy.Results:iPSCs differentiated cells had similar morphology,phenotype,and gene expression to epidermal stem cells.The cells grew in a tight and thin sheet like manner on the acellular amniotic membrane,and had significant differences in proliferation compared to the control group.Conclusion:iPSCs derived epidermal stem cells have good biocompatibility with acellular amniotic membrane and are potential seed cells for skin substitutes.

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备注/Memo

备注/Memo:
基金项目:浙江省医药卫生科技计划项目(2021438727)
杭州市萧山区重大科技计划项目(2020213)
更新日期/Last Update: 2024-05-15