[1]李贺伟,吴燕茹,阮锋△,等.杜鹃素上调靶向基因表达抑制白细胞介素-1β诱导的软骨细胞损伤[J].中国中医骨伤科杂志,2022,30(09):14-19.
 LI Hewei,WU Yanru,RUAN Feng,et al.Study of Farrerol on the Inhibition of IL-1β-induced Chondrocyte Injury by Upregulating miR-499a-5p Expression[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2022,30(09):14-19.
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杜鹃素上调靶向基因表达抑制白细胞介素-1β诱导的软骨细胞损伤()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第30卷
期数:
2022年09期
页码:
14-19
栏目:
实验研究
出版日期:
2022-09-15

文章信息/Info

Title:
Study of Farrerol on the Inhibition of IL-1β-induced Chondrocyte Injury by Upregulating miR-499a-5p Expression
文章编号:
1005-0205(2022)09-0014-06
作者:
李贺伟1吴燕茹2阮锋1△赵映松3张光恒3
1华中科技大学同济医学院附属梨园医院骨科(武汉,430077)
2华中科技大学同济医学院附属梨园医院病理科
3华中科技大学同济医学院附属梨园医院中心实验室
Author(s):
LI Hewei1WU Yanru2RUAN Feng1△ZHAO Yingsong3ZHANG Guangheng3
1Department of Orthopedics,Liyuan Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430077,China; 2Department of Pathology,Liyuan Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430077,China; 3Central Laboratory of Liyuan Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430077,China.
关键词:
软骨细胞 杜鹃素 miR-499a-5p 细胞凋亡 炎症
Keywords:
chondrocytes farrerol miR-499a-5p apoptosis inflammation
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨杜鹃素对白细胞介素-1β(IL-1β)诱导的软骨细胞损伤的影响及其可能作用机制。方法:IL-1β诱导人正常软骨细胞建立细胞损伤模型,不同浓度的杜鹃素处理软骨细胞,miR-NC、miR-499a-5p mimics分别转染至软骨细胞后加入10 ng/mL IL-1β处理24 h,anti-miR-NC、anti-miR-499a-5p分别转染至软骨细胞后加入90 μmol/L杜鹃素与10 ng/mL IL-1β共同处理24 h; ELISA法检测IL-6、TNF-α、IFN-γ的水平; 流式细胞术检测细胞凋亡率; qRT-PCR法检测miR-499a-5p的表达量; Western Blot检测凋亡相关蛋白表达量。结果:杜鹃素处理后IL-1β诱导的软骨细胞中IL-6、TNF-α、IFN-γ的水平降低(P<0.05),凋亡率、Cleaved-caspase3、Cleaved-caspase9蛋白水平降低(P<0.05),miR-499a-5p的表达量升高(P<0.05),且呈剂量依赖性; 转染miR-499a-5p mimics后IL-6、TNF-α、IFN-γ的水平降低(P<0.05),凋亡率、Cleaved-caspase3、Cleaved-caspase9蛋白水平降低(P<0.05); 转染anti-miR-499a-5p可降低杜鹃素对IL-1β诱导的软骨细胞炎症反应和凋亡的影响。结论:杜鹃素可通过增加miR-499a-5p表达而抑制炎症反应及细胞凋亡从而减轻IL-1β诱导的软骨细胞损伤。
Abstract:
Objective:To explore the efficacy of farrerol on interleukin-1β(IL-1β)-induced chondrocyte damage and its possible mechanism.Methods:Human normal chondrocyte was induced by IL-1β to establish a cell injury model.Chondrocyte was treated by different concentrations of farrerol.The miR-NC and miR-499a-5p mimics were transfected into chondrocytes and treated with 10 ng/mL IL-1β for 24 h.The anti-miR-NC and anti-miR-499a-5p were transfected into chondrocytes and treated with 90 μmol/L farrerol and 10 ng/mL IL-1β for 24 h.ELISA method was used to detect the levels of IL-6,TNF-α,IFN-γ.Flow cytometry was used to detect the rate of apoptosis; qRT-PCR was used to detect the expression of miR-499a-5p.Western Blot was used to detect the protein expression of cleaved-caspase3 and cleaved-caspase9.Results:After farrerol treatment,the levels of IL-6,TNF-α and IFN-γ in chondrocytes induced by IL-1β were decreased(P<0.05),and the apoptosis rate and the protein levels of cleaved-caspase3,cleaved-caspase9 were decreased(P<0.05),while the expression of miR-499a-5p was increased(P<0.05),and was dose-dependent.After transfection of miR-499a-5p mimics,the levels of IL-6,TNF-α,and IFN-γ were decreased(P<0.05),and the rate of cell apoptosis and the protein levels of cleaved-caspase3,cleaved-caspase9 were decreased(P<0.05).Transfection of anti-miR-499a-5p could reduce the effect of farrerol on IL-1β-induced inflammation and apoptosis of chondrocytes.Conclusion:Farrerol could inhibit the inflammation and apoptosis by up-regulating the expression of miR-499a-5p,thereby reducing IL-1β-induced chondrocyte injury.

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备注/Memo

备注/Memo:
基金项目:武汉市中青年医学骨干人才项目(武卫通〔2019〕87号)
通信作者 E-mail:a787qm@163.com
更新日期/Last Update: 2022-09-10