[1]陈佩 贾志姣 陈瑨.大黄素抗小鼠皮肤移植排斥反应的实验研究[J].中国中医骨伤科杂志,2019,27(08):5-8.
　CHEN Pei JIA Zhijiao CHEN Jin.Effects of Emodin on Experimental Skin Transplantation of Mice[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2019,27(08):5-8.
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大黄素抗小鼠皮肤移植排斥反应的实验研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第27卷
期数:
2019年08期
页码:
5-8
栏目:
实验研究
出版日期:
2019-07-30

文章信息/Info

Title:
Effects of Emodin on Experimental Skin Transplantation of Mice
文章编号:
1005-0205(2019)08-0005-04
作者:
陈佩1 贾志姣2 陈瑨3
1武警北京市总队医院医学工程科(北京,100027) 2北京中医药大学东方医院 3武警北京市总队医院内一科
Author(s):
CHEN Pei1 JIA Zhijiao2 CHEN Jin3△
1Medical Engineering Department,Hospital of Beijing Armed Police Forces,Beijing 100027,China; 2Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China; 3First Department of Internal Medicine,Hospital of Beijing Armed Police Forces,Beijing 100027,China.
关键词:
大黄素;  免疫抑制;  皮肤移植
Keywords:
emodin;  immunosuppressive effect;  skin transplantation
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨大黄素抑制同种异体小鼠皮肤移植排斥反应的作用。方法:采用BALB/c供体鼠 → C57BL/6受体鼠背-背皮肤移植法建立模型,分为假手术组、模型组、大黄素组(50 mg/kg)、环孢素A组(10 mg/kg)、大黄素+亚剂量环孢素A组(大黄素 50 mg/kg+环孢素A 5 mg/kg),腹腔注射给药10 d.记录受体鼠移植皮片的存活时间; 取血浆,酶联免疫吸附法检测Th1类细胞因子(IL-2,IFN-γ)和Th2类细胞因子(IL-4,IL-10)水平; 取脾脏,流式细胞仪分析T淋巴细胞亚群CD4+及CD25+ 的比例。结果:与模型组比较,各给药组受体鼠移植皮片的存活时间均有明显延长,组间差异均有统计学意义(P<0.01); 大黄素联合环孢素A组的移植皮片存活时间比大黄素组或环孢素A组均有明显延长,差异有统计学意义(P<0.05)。与模型组比较,大黄素联合环孢素A组及大黄素组均可显著降低血浆IL-2和IFN-γ的水平,差异有统计学意义(P<0.01),显著升高IL-4和IL-10的水平,差异有统计学意义(P<0.01)。与模型组比较,大黄素联合环孢素A组及大黄素组均可显著升高脾脏CD4+及CD25+调节性T细胞的比例,差异有统计学意义(P< 0.01)。结论:大黄素可抑制同种异体小鼠的皮肤移植排斥反应,其机制或与促使Thl向Th2细胞的偏移,刺激CD4+及CD25+调节性T细胞的表达,诱导免疫耐受等相关。
Abstract:
Objective:To investigate the effects of emodin against mice with xenogenic skin transplantation.Methods: The tergal skin transplantation model was achieved by an operation(BALB/c mice as donors and C57BL/6 mice as acceptors,respectively).Then acceptors were divided randomly into 5 groups,namely sham,model,emodin(50 mg/kg),ciclosporin A(10 mg/kg),combination of emodin and ciclosporin A(emodin 50 mg/kg and ciclosporin A 5 mg/kg).All acceptor mice were intraperitoneally administered 10 d.The skin graft survival time of all mice were documented every day.At the end of the experiment,the levels of Th1 cytokines(IL-2 and IFN-γ)and Th2 cytokines(IL-4 and IL-10)in plasma were detected by ELISA and the spleen in all mice were removed and quantity of CD4+,CD25+ T cells were analysed by flow cytometry.Results: Compared with the survival days of skin graft of model group,those in groups treated with drugs were all significantly prolonged(P<0.01).Combination of emodin and ciclosporin A group showed a longer survival days than emodin or ciclosporin A group(P<0.05).IL-2 and IFN-γ levels in plasma in groups treated with emodin or combination of emodin and ciclosporin A were evidently decreased(P<0.01)while IL-4 and IL-10 levels were evidently increased(P<0.01)comparing with those of model group.Compared with the amount of CD4+,CD25+ T cells in spleen of model group,those in groups treated with emodin or combination of emodin and ciclosporin A were all significantly increased(P<0.01).Conclusion: Emodin has an immunosuppressive effect against mice with xenogenic skin transplantation.The effectiveness of emodin appears to be associated with balance of Th1/Th2 cytokines and increasement of expression of CD4+,CD25+ T cells,etc.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(81773859)通信作者 E-mail:PC2005CP@163.com(收稿日期:2019-04-06)
更新日期/Last Update: 1900-01-01