[1]李强 张亚斌 赵秋芳 李良.萆薢总皂苷对骨关节炎体外软骨细胞增殖及炎性因子的影响[J].中国中医骨伤科杂志,2019,27(08):1-4.
 LI Qiang ZHANG Yabin ZHAO Qiufang LI Liang.Effects of Total Saponins of Involucate on Proliferation and Inflammatory Factors of Osteoarthritis in Vitro[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2019,27(08):1-4.
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萆薢总皂苷对骨关节炎体外软骨细胞增殖及炎性因子的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第27卷
期数:
2019年08期
页码:
1-4
栏目:
实验研究
出版日期:
2019-07-30

文章信息/Info

Title:
Effects of Total Saponins of Involucate on Proliferation and Inflammatory Factors of Osteoarthritis in Vitro
文章编号:
1005-0205(2019)08-0001-04
作者:
李强1 张亚斌1 赵秋芳1 李良1
陕西省第四人民医院(西安,710043)
Author(s):
LI Qiang1 ZHANG Yabin1 ZHAO Qiufang1 LI Liang1△
1The Fourth People’s Hospital of Shaanxi,Xi’an 710043,China.
关键词:
萆薢总皂苷 骨关节炎 增殖 炎性因子
Keywords:
total saponins of rabdosia involucate(TRI) osteoarthritis(OA) proliferation inflammatory factors
分类号:
R-33
文献标志码:
A
摘要:
目的:观察萆薢总皂苷(TRI)含药关节液对豚鼠骨关节炎(OA)体外软骨细胞增殖及炎性因子的影响。方法:将30只豚鼠进行OA造模,OA造模成功后体外提取关节软骨细胞进行培养、鉴定。将 P3代软骨细胞分为TRI组、阳性药物牛膝总皂苷组(TSA)和空白对照组,分别用TRI+10% FBS+DMEM:F12(1:1)和10% FBS+DMEM:F12(1:1)培养液进行培养,对培养后的软骨细胞进行细胞形态学观察,用CCK-8法检测细胞增殖情况,免疫荧光检测Ⅱ型胶原蛋白表达,酶联免疫吸附测定(ELISA)检测细胞白细胞介素 1(IL-1)、肿瘤坏死因子α(TNF-α)和基质金属蛋白酶13(MMP-13)。结果:OA软骨细胞体外分离成功,经甲苯胺蓝染色和细胞电镜观察鉴定细胞为来源于软骨细胞; CCK-8法观察3组细胞生长曲线,TRI组在第2~6天细胞增殖速度明显高于其他2组,差异有统计学意义(P<0.05); 连续培养7 d后,与空白对照组和TSA组比较,TRI组Ⅱ型胶原蛋白表达显著,差异有统计学意义(P<0.05); ELISA检测显示,与空白对照组和TSA组比较,TRI组细胞炎性因子IL-1,TNF-α和MMP-13炎性因子显著减少,差异有统计学意义(P<0.05)。结论:TRI含药关节液能有效提高细胞活力,促进软骨细胞增殖,提高Ⅱ型胶原蛋白表达,且降低软骨细胞炎性因子的表达,对证实TRI是萆薢作用于软骨细胞的主要物质基础具有重要意义。
Abstract:
Objective: To observe the effects of total saponins of rabdosia involucate(TRI)on the proliferation of chondrocytes and inflammatory factors in guinea pig osteoarthritis(OA).Methods: Thirty guinea pigs were subjected to OA modeling,and the articular chondrocytes were cultured and identified in vitro after successful OA modeling.P3 chondrocytes were divided into TRI group,positive drug total saponins of achyranthes(TSA)and blank control group(control); TRI+10% FBS+DMEM:F12(1:1)and 10% FBS+DMEM:F12(1:1)medium was cultured,the morphology of the cultured chondrocytes was observed,the cell proliferation was detected by CCK-8 method,and the expression of type Ⅱ collagen was detected by immunofluorescence interleukin-1(IL-1),tumor necrosis factor(TNF-α)and matrix metalloproteinase(MMP-13).Results: The OA chondrocytes were successfully isolated in vitro.The cells were identified as chondrocytes by toluidine blue staining and cell electron microscopy.The growth curves of the three groups were observed by CCK-8 method,and the growth curve was drawn.The cell proliferation rate of the TRI group on days 2~6 compared with the other two groups,the difference was statistically significant(P<0.05).After 7 d of continuous culture,the expression of type Ⅱ collagen in TRI group was significantly compared with the blank control group and TSA group,the difference was statistically significant(P< 0.05); ELISA showed that compared with the blank control group and TSA group,the inflammatory factors IL-1,TNF-α and MMP-13 inflammatory factors in the TRI group were significantly decreased,the difference was statistically significant(P<0.05).Conclu-sion: TRI-containing articular fluid can effectively improve cell viability,promote chondrocyte proliferation,increase collagen type Ⅱ protein expression,and reduce the secretion of inflammatory factors in chondrocyte.It is of great significance to study that TRI is the main material basis of chondrocyte action by razor.

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备注/Memo

备注/Memo:
基金项目:陕西省自然科学基金面上项目(2017JM8034) 通信作者 E-mail:285747843@qq.com(收稿日期:2019-04-07)
更新日期/Last Update: 1900-01-01