[1]李永志,董博△,欧国峰,等.骨复生对激素性股骨头坏死大鼠骨组织中OPG及RANK表达的影响[J].中国中医骨伤科杂志,2018,26(11):1-6.
 LI Yongzhi,DONG Bo,OU Guofeng,et al.Effect of Gufusheng on the Expression of OPG and RANK in Bone Tissue of Rats with Steroid-induced Femoral Head Necrosis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2018,26(11):1-6.
点击复制

骨复生对激素性股骨头坏死大鼠骨组织中OPG及RANK表达的影响()
分享到:

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第26卷
期数:
2018年11期
页码:
1-6
栏目:
实验研究
出版日期:
2018-10-30

文章信息/Info

Title:
Effect of Gufusheng on the Expression of OPG and RANK in Bone Tissue of Rats with Steroid-induced Femoral Head Necrosis
文章编号:
1005-0205(2018)11-0001-06
作者:
李永志1董博2△欧国峰3朱峰峰2袁普卫2刘继华2王国柱3肖斌3史国号3霍洪亮3
1陕西省商洛市中医医院(陕西 商洛,726000) 2陕西中医药大学附属医院 3陕西中医药大学第二附属医院
Author(s):
LI Yongzhi1DONG Bo2△OU Guofeng3ZHU Fengfeng2YUAN Puwei2LIU Jihua2WANG Guozhu3XIAO Bin3SHI Guohao3HUO Hongliang3
1Shangluo City Hospital of Traditional Chinese Medicine, Shangluo 726000, Shaanxi China; 2Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, Xianyang 712000,Shaanxi China; 3Second Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, Xianyang 712000,Shaanxi China.
关键词:
骨复生 激素性股骨头坏死 骨保护素 受体活化因子 实验研究
Keywords:
Gufusheng steroid-induced femoral head necrosis osteoprotegerin(OPG) receptor activator of nuclear factor-kB(RANK) experimental study
分类号:
R-33
文献标志码:
A
摘要:
目的:基于动物模型探讨骨复生对激素性股骨头坏死大鼠骨保护素(OPG),受体活化因子(RANK)信号通路的影响。方法:将80只大鼠随机分为正常组、模型组、中药组、西药组4组,每组各20只。除正常组外其余各组连续2 d中每日经尾静脉注射40 μg/kg的脂多糖,之后每隔3 d臀部肌肉(所有动物统一从右侧臀大肌以45°角匀速注射)注射地塞米松25 mg/kg,连续注射6周。观察造模前后以及治疗前后的股骨头X线变化、组织病理学改变以及OPG,NF-κB基因表达情况。结果:1)中药组、西药组双侧股骨头X线结果与正常组最为接近,股骨头大小形态正常,骨密度均匀,骨小梁清晰,但西药组仍有少部分密度不均匀; 模型组股骨头密度不均,但股骨头仍完整,偶见透亮囊状阴影。2)正常组细胞结构清晰,空骨陷窝较少见; 中药组可见骨细胞大部分正常,空骨陷窝较少; 西药组变化大致与中药组接近,但空骨陷窝较中药组多; 模型组细胞结构紊乱、细胞萎缩、细胞集聚固缩,空骨陷窝明显存在。3)中药组和西药组分别与模型组比较,中药组和西药组骨组织中RANK的表达均下调,且中药组下调更明显,而中药组和西药组骨组织中OPG表达均上调,差异有统计学意义(P<0.05); 中药组和西药组与正常组比较,骨组织中OPG,RANKL及RANK的表达差异无统计学意义(P>0.05); 中药组与西药组比较,骨组织中OPG的表达差异有统计学意义(P<0.05)。结论:骨复生可明显上调OPG的表达,下调RANK的表达,增加骨细胞形成,促进骨量上升,减少骨钙流失,促进骨质成熟,改善股骨头局部缺血缺氧环境,从而有效干预激素性股骨头坏死的形成。
Abstract:
Objective:The effects of Gufusheng on osteoprotegerin(OPG)and receptor activator of nuclear factor-kB(RANK)signaling pathways in rats with steroid-induced femoral head necrosis were investigated based on animal models.Methods:Eighty rats were randomly divided into normal group, model group, Chinese medicine group and western medicine group, with 20 rats in each group.Except for the normal group, the other groups were injected with 40 μg/kg lipopolysaccharide via caudal vein daily for 2 d, then dexamethasone 25 mg/kg was injected into the gluteal muscles every 3 d(all animals were injected uniformly from the right gluteal muscles at 45°)for 6 weeks.The X-ray changes, histopathological changes and the expression of OPG and NF-kB genes were observed before and after modeling and treatment.Results:1)The X-ray results of the bilateral femoral heads in the Chinese medicine group and the western medicine group were the closest to the normal group.The size and shape of the femoral head were normal, the bone density was uniform, and the trabecular bone was clear, but the western medicine group still had a small density unevenness.The density of femoral head in the model group was not uniform, but the femoral head was still intact, occasionally with bright cystic shadow.2)The cell structure of the normal group was clear, and the empty bone lacuna was less common; the Chinese medicine group showed that most of the bone cells were normal, and the empty bone lacuna was less; the change of the western medicine group was similar to that of the traditional Chinese medicine group, but the empty bone lacuna was more than the traditional Chinese medicine group; The model group had cell structure disorder, cell atrophy, cell agglomeration and pyknosis, and the number of empty bone sag was obvious.3)Compared with the model group, the expressions of RANK in the Chinese medicine group and the western medicine group were down-regulated, and the traditional Chinese medicine was used.The down-regulation of the group was more obvious, while the expression of OPG in the bone tissue of the Chinese medicine group and the western medicine group was up-regulated(P<0.05).There was no difference in the expression of OPG, RANKL and RANK between the Chinese medicine group and the western medicine group compared with the normal group(P>0.05).The expression of OPG in bone tissue was significantly different between Chinese medicine group and western medicine group(P<0.05).Conclusion: The results of this study indicate that bone rejuvenation can significantly up-regulate the expression of OPG, down-regulate the expression of RANK, increase bone cell formation, promote bone mass increase, reduce bone calcium loss, promote bone maturation, and effectively improve the ischemic and anoxic environment of the femoral head.Thereby effectively intervening in the formation of steroid-induced femoral head necrosis.

参考文献/References:

[1] Gagala J,Tarczynska M,Gaweda K.Clinical and radiological outcomes of treatment of avascular necrosis of the femoral head using autologous osteochondral transfer(mosaicplasty):preliminary report[J].Int Orthop,2013,37(7):1239-1244.
[2] Signorino JA,Jayaseelan DJ,Brindle K.A typical clinical presentation of rapidly progressing femoral head avascular necrosis[J].J Orthop Sports Phys Ther,2017,47(3):217.
[3] Liu L,Gao F,Sun W,et al.Investigating clinical failure of core decompression with autologous bone marrow mononuclear cells grafting for the treatment of non-traumatic osteonecrosis of the femoral head[J].Int Orthop,2018,42(7):1575-1583.
[4] 李泰贤,陈志伟,王荣田,等.基于文献计量学分析中医药治疗股骨头坏死的研究现状[J].中国中医骨伤科杂志,2017,25(4):41-46.
[5] Zhao L,Kaye AD,Kaye AJ,et al.Stem cell therapy for osteonecrosis of the femoral head:current trends and comprehensive review[J].Curr Pain Headache Rep,2018,22(6):41.
[6] Song HM,Wei YC,Li N,et al.Effects of Wenyangbushen formula on the expression of VEGF,OPG,RANK and RANKL in rabbits with steroid-induced femoral head avascular necrosis[J].Mol Med Rep,2015,12(6):8155-8161.
[7] 徐西林,胡海,张晓峰,等.活骨注射液治疗股骨头坏死的扫描电镜观察[J].中国中医骨伤科杂志,2017,25(2):1-5.
[8] 袁普卫,贺西京,王国毓,等.骨复生胶囊治疗早期儿童股骨头缺血性坏死的临床观察[J].中国中西医结合杂志,2005,25(4):330-332.
[9] 袁普卫,贺西京,周海哲,等.骨复生胶囊对实验性激素性股骨头缺血坏死家兔IGF-1的影响[J].中国中医骨伤科杂志,2007,15(2):40-41.
[10] 袁普卫,刘德玉,贺西京,等.中药复方骨复生对激素性股骨头缺血坏死家兔TNF-α的影响[J].中国骨伤,2004,17(11):662-664.
[11] 贺西京,毛履真,王坤正,等.肾上腺皮质激素引起股骨头缺血性坏死的机制实验研究[J].中华骨科杂志,1992,12(6):440.
[12] 董国华,邱红明.邱红明治疗股骨头坏死经验[J].湖南中医杂志,2017,33(3):34-36.
[13] 邓海军.中医综合疗法治疗股骨头坏死的疗效分析[J].黑龙江中医药,2015,44(6):22-23.
[14] Caplan A,Fett N,Rosenbach M,et al.Prevention and management of glucocorticoid-induced side effects:a comprehensive review:a review of glucocorticoid pharmacology and bone health[J].Journal of the American Academy of Dermatology,2017,76(1):1-9.
[15] Hauzeur JP,Toungouz M,Lechanteur C,et al.Autologous osteoblastic cells(PREOBy)versus concentrated bone marrow implantation in osteonecrosis of the femoral head:a randomized study[J].Revue de Chirurgie Orthopédique et Traumatologique,2016,102(7):S73.
[16] Cui L,Zhuang Q,Lin J,et al.Multicentric epidemiologic study on six thousand three hundred and ninety five cases of femoral head osteonecrosis in China[J].International orthopaedics,2016,40(2):267-276.
[17] Johnson AJ,Mont MA,Tsao AK,et al.Treatment of femoral head osteonecrosis in the United States:16-year analysis of the nationwide inpatient sample[J].Clinical Orthopaedics and Related Research,2014,472(2):617-623.
[18] 康武林,袁普卫.股骨头坏死与骨髓间充质干细胞移植研究进展[J].中国矫形外科杂志,2013,21(19):1954-1957.
[19] 吉万波,刘冠虹,刘锦涛,等.股密葆方对大鼠激素性股骨头坏死血管修复影响的实验研究[J].中国骨质疏松杂志,2014,20(10):1148-1153.
[20] 周李学,李志敏,段璋.活血法防治早期激素性股骨头坏死的研究进展[J].创伤与急诊电子杂志,2015,3(2):1-5.
[21] 陆启恩.股骨头坏死的病因分析及治疗进展[J].实用医技杂志,2014,21(1):44-45.
[22] 罗丹,宋振华,胡建庚,等.补气活血法治疗气虚血瘀型股骨头坏死23例[J].中国中医药现代远程教育,2016,14(2):74-75.
[23] 徐复朕,尹纪光.启功散加减治疗早期股骨头缺血性坏死50例疗效观察[J].湖南中医杂志,2016,32(11):73-74.
[24] 柳锦华,刘建卫.补肾活血汤治疗中老年激素性股骨头坏死的临床效果分析[J].齐齐哈尔医学院学报,2016,37(33):4174-4176.
[25] 章建华,尹华,林婷,等.芪参健骨颗粒对激素性股骨头坏死大鼠骨组织的影响[J].中国中医骨伤科杂志,2014,22(1):1-4..
[26] 陈镇秋,何伟,魏秋实.股骨头坏死中医证型与血液学指标的相关性研究[J].中华中医药杂志,2013,28(7):2205-2208.
[27] 芮丹云,唐丽媛,陈洁,等.补肾活血颗粒对骨质疏松大鼠骨组织中OPG/RANKL mRNA及蛋白表达的影响[J].中国老年学杂志,2015,35(24):7036-7038.
[28] 宋红梅,魏迎辰,吴斌,等.温阳补肾方对兔激素性股骨头坏死组织RANKL/RANK/OPG通路的影响[J].中华中医药杂志,2016,31(1):302-305.

备注/Memo

备注/Memo:
基金项目:陕西省咸阳市科学技术研究计划项目(2017K02-76) 陕西省中医管理局中医药科研项目(15-SCJH004) 国家中医药管理局国家中医临床研究基地业务建设 科研专项(2JDZX2015280) 陕西省重点科技创新团队项目(2013KJT-26) 全国名老中医药专家刘德玉传承工作室建设资助项目
通信作者 E-mail:297065203@qq.com
更新日期/Last Update: 2018-10-10