[1]张军 黄楠 武永刚 齐明.骨炎方抗家兔慢性骨髓炎的实验研究[J].中国中医骨伤科杂志,2018,26(09):14-18.
　ZHANG Jun HUANG Nan WU Yonggang QI Ming.Experimental Study of Guyan Prescription on Chronic Osteomyelitis in Rabbits[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2018,26(09):14-18.
点击复制

骨炎方抗家兔慢性骨髓炎的实验研究()
分享到：

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第26卷
期数:
2018年09期
页码:
14-18
栏目:
实验研究
出版日期:
2018-09-06

文章信息/Info

Title:
Experimental Study of Guyan Prescription on Chronic Osteomyelitis in Rabbits
文章编号:
1005-0205(2018)09-0014-05
作者:
张军1 黄楠1 武永刚1 齐明1
1武警北京市总队第二医院外二科(北京,100027)
Author(s):
ZHANG Jun1 HUANG Nan1 WU Yonggang1 QI Ming1
1Second Hospital of Beijing Armed Police Forces,Beijing 100027,China.
关键词:
骨炎方;  兔;  骨髓炎;  溶菌酶;  炎性细胞因子
Keywords:
Keywords: Guyan prescription;  rabbit;  osteomyelitis;  lysozyme;  inflammatory cytokines
分类号:
R-33
文献标志码:
A
摘要:
目的:观察骨炎方对家兔慢性骨髓炎的作用。方法:家兔右胫骨骨髓腔内注射金黄色葡萄球菌建立慢性骨髓炎模型,分为正常对照组、模型组、庆大霉素组(60 mg/kg)、骨炎方高剂量组(生药3.6 g/kg)、骨炎方低剂量组(生药1.8 g/kg),每组10只,灌胃给药,术后第6周对右胫骨行X-线检查并做Laurence评分; 术后第2,4及6周采血测定白细胞计数、血清溶菌酶、血清TNF-α和IL-6含量。结果:模型组家兔均有典型骨髓炎表现,骨炎方高、低剂量组对家兔骨髓炎症状有显著缓解,Laurence评分与模型组比较差异均有统计学意义(P值分别为0.000,0.001); 与模型组比较,骨炎方高剂量组白细胞计数在第2,4及6周均有明显降低,差异有统计学意义(P值分别为0.015,0.012,0.001),低剂量组白细胞计数在第2,4及6周均有明显降低,差异有统计学意义(P值分别为0.013,0.008,0.036),高剂量组血清溶菌酶含量在第2,4及6周均有明显升高,差异有统计学意义(P值分别为0.033,0.002,0.003),低剂量组血清溶菌酶含量在第2,4及6周亦有明显升高,差异有统计学意义(P值分别为0.048,0.028,0.024),高剂量组血清TNF-α含量在第2,4及6周均有明显降低,差异有统计学意义(P值分别为0.031,0.003,0.000),低剂量组血清TNF-α含量在第2,4及6周均有明显降低,差异有统计学意义(P值分别为0.032,0.008,0.000),高剂量组血清IL-6含量在第2,4及6周均有明显降低,差异有统计学意义(P值分别为0.004,0.000,0.000); 低剂量组血清IL-6含量在第2,4及6周均有明显降低,差异有统计学意义(P值分别为0.009,0.000,0.000).结论:骨炎方对家兔慢性骨髓炎的防护作用可能与抑制致病菌、提高机体免疫力、下调炎性因子等途径有关。
Abstract:
Abstract Objective:To investigate the effects of Guyan prescription(GP)against chronic osteomyelitis in rabbits.Methods:Chronic osteomyelitis model of rabbits were prepared by intra-shinbone marrow injection of Staphylococcus aureus.Fifty rabbits were divided randomly into five groups(n=10),i.e.normal,model,gentamicin(60 mg/kg),GP high and low dose groups(3.6 and 1.8 g/kg,respectively).Drugs were orally administrated after the operation.X-ray examination of the right shinbone at the sixth week after operation was done and Laurence score were calculated.At the second,fourth and sixth week after operation,blood was collected to determine the leukocyte count,serum lysozyme,serum TNF-α and IL-6 levels.Results:Rabbits in the model group all showed typical osteomyelitis.The high- and low-dose groups of GP significantly alleviated the symptoms of osteomyelitis in the rabbits,and the Laurence scores were statistically significant compared with the model group(P values were 0.000,0.001,respectively).Compared to model group,leucocyte count of the high-dose GP group had a significant decrease at the second,fourth and sixth weeks(P=0.015,0.012,0.001),so did the low-dose GP group(P=0.013,0.008 and 0.036,respectively).Serum lysozyme levels increased significantly both in the high-dose group(P values were 0.033,0.002,and 0.003,respectively)and low dose group(P=0.048,0.028 and 0.024,respectively)at the 2nd,4th,and 6th weeks.The levels of serum TNF-α both in the high-dose group and in the low-dose group(P=0.032,0.008 and 0.000,respectively)decreased significantly at the 2nd,4th,and 6th week,and the difference was statistically significant(P values were 0.031,0.003,and 0.000,respectively).The serum IL-6 levels both in the high-dose group(P values were 0.004,0.000,and 0.000,respectively)and in the low-dose group(P values were 0.009,0.000,0.000 respectively)were significantly lower at the second,fourth,and sixth weeks.Conclusion:The protective effect of GP on osteomyelitis seems to be associated with inhibition of pathogenic bacteria,improvement of immune function and down regulation of inflammatory cytokines.

参考文献/References:

[1] Walter G,Kemmerer M,Kappler C,et al.Treatment algorithms for chronic osteomyelitis[J].Dtsch Arztebl Int,2012,109(14):257-264.
[2] Dym H,Zeidan J.Microbiology of acute and chronic osteomyelitis and antibiotic treatment[J].Dent Clin North Am,2017,61(2):271-282.
[3] 韩宗其,左国营,郝小燕.中药成分联合抗生素抗MRSA作用的研究进展[J].中草药,2012,43(12):187-195.
[4] 顾卓珺,顾维凤,吴飞跃,等.医院感染病原菌中药与抗菌药物联合治疗的研究[J].中华医院感染学杂志,2015,25(10):2236-2238.
[5] Strelkov NS,Kiryanov NA,Shklyaev PO,et al.Pathologic morphology of acute experimental osteomyelitis[J].Bosn J Basic Med Sci,2013,13(3):153-157.
[6] Dahners LE,Funderburk CH.Gentamicin-loaded plaster of Paris as a treatment of experimental osteomyelitis in rabbits[J].Clin Orthop Relat Res,1987,219(3):278-282.
[7] Kalinka J,Hachmeister M,Geraci J,et al.Staphylococcus aureus isolates from chronic osteomyelitis are characterized by high host cell invasion and intracellular adaptation,but still induce inflammation[J].Int J Med Microbiol,2014,304(8):1038-1049.
[8] Ulug M,Ayaz C,Celen MK.A case report and literature review:osteomyelitis caused by community-associated methicillin resistant Staphylococcus aureus[J].J Infect Dev Ctries,2011,5(12):896-900.
[9] 刘振云.中医三联疗法治疗慢性骨髓炎200例[J].湖南中医杂志,2014,30(8):95-96.
[10] 崔玉莹,王宪贝,李玉坤,等.金银花、连翘水煎液对金黄色葡萄球菌的抑制作用研究[J].光明中医,2017,32(18):2637-2638.
[11] 霍佳楠,姜晓文,许晶,等.蒲公英水提物对体外金黄色葡萄球菌生物膜形成的影响[J].中兽医医药杂志,2015(1):12-15.
[12] 杨明炜,陆付耳,徐丽君,等.紫花地丁对耐甲氧西林金黄色葡萄球菌耐药质粒的消除作用[J].中西医结合研究,2009,1(1):27-28.
[13] 陈柳萌,曹树稳,余燕影.白花蛇舌草-半枝莲药对提取物抑菌活性部位研究[J].时珍国医国药,2011,22(1):93-95.
[14] 郑险峰,张金艳,邵淑娟,等.白头翁对金黄色葡萄球菌和产气肠杆菌抑菌作用的研究[J].牡丹江师范学院学报:自然科学版,2011(2):29-30.
[15] 周秀萍,李争鸣,刘志杰,等.金银花对大鼠免疫功能影响的研究[J].实用预防医学,2011,18(2):214-216.
[16] 高慧婕,李晓,李春霞,等.蒲公英多糖对免疫抑制小鼠的免疫增强作用及对TNF-α的调节[J].济宁医学院学报,2016,39(6):391-394.
[17] 宋高臣,于英君,王喜军.半枝莲多糖的抗肿瘤作用及其调节免疫的实验研究[J].世界科学技术-中医药现代化,2011,13(4):641-643.
[18] 杨小龙,李世朋,王朏朏,等.白头翁醇提取物对小鼠免疫功能的影响[J].河南科技大学学报:医学版,2011,29(4):249-250.
[19] 孟玮,刘志强,邱世翠,等.中药白花蛇舌草对小鼠免疫功能影响的初步研究[J].现代中西医结合杂志,2005,14(2):163-164.
[20] 沈芃.溶菌酶抗菌作用及其在防控医院感染中的应用[J].中国消毒学杂志,2014,31(10):1089-1091.
[21] Novak ML,Koh TJ.Macrophage phenotypes during tissue repair[J].J Leukoc Biol,2013,93(6):875-881.
[22] Jiang N,Qin CH,Hou YL,et al.Serum TNF-α,erythrocyte sedimentation rate and IL-6 are more valuable biomarkers for assisted diagnosis of extremity chronic osteomyelitis[J].Biomark Med,2017.
[23] Jiang N,Ma YF,Jiang Y,et al.Clinical characteristics and treatment of extremity chronic osteomyelitis in southern china:a retrospective analysis of 394 consecutive patients[J].Medicine(Baltimore),2015,94(42):e1874.

更新日期/Last Update: 2018-09-06