[1]胡中青 汤样华△ 曾林如 郑文杰 岳振双 熊振飞.右归丸治疗骨质疏松性骨缺损的机制研究[J].中国中医骨伤科杂志,2018,26(07):11-15,20.
　HU Zhongqing TANG Yanghua ZENG Linru ZHENG Wenjie YUE Zhenshuang XIONG Zhenfei.Study on the Mechanism of Yougui Pill for Treating Osteoporosis Bone Defect[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2018,26(07):11-15,20.
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右归丸治疗骨质疏松性骨缺损的机制研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第26卷
期数:
2018年07期
页码:
11-15,20
栏目:
实验研究
出版日期:
2018-07-02

文章信息/Info

Title:
Study on the Mechanism of Yougui Pill for Treating Osteoporosis Bone Defect
文章编号:
1005-0205(2018)07-0011-05
作者:
胡中青1 汤样华1△ 曾林如1 郑文杰1 岳振双1 熊振飞1
1杭州市萧山区中医院(杭州,311201) △通信作者 E-mail:tangyanghua168@163.com
Author(s):
HU Zhongqing1 TANG Yanghua1△ ZENG Linru1 ZHENG Wenjie1 YUE Zhenshuang1 XIONG Zhenfei1
1Hangzhou Xiaoshan District Hospital of Traditional Chinese Medicine,Hangzhou 311201,China.
关键词:
右归丸;  β-链蛋白;  调控骨髓基质干细胞;  骨质疏松性骨缺损
Keywords:
Keywords: Yougui pill;  β-catenin;  bone mesenchymal stem cells;  osteoporotic bone defect
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨右归丸介导β-链蛋白(β-catenin)信号通路调控骨髓基质干细胞(BMSCs)治疗骨质疏松性骨缺损的机制。方法:进行小鼠骨髓间充质干细胞分离和体外扩增,鉴定其生物学活性。选择6月龄小鼠,建立骨质疏松性骨缺损模型,并给予右归丸[(18.9 g/(kg·d)]进行治疗6周。采用酶联免疫吸附法(Elisa)检测小鼠血清中碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRAP)、血清骨钙素(BGP)和雌二醇(E2); 采用免疫组织化学法检测β-Catenin表达; 采用实时PCR法检测骨钙素(Osteocalcin)、骨桥蛋白(Osteopontin)、成骨细胞特异性转录因子(Osterix)、骨细胞的特异转录因子(Runx2/Cbfa1)和β-Catenin mRNA表达。结果:与模型组相比,右归丸组和右归丸+BMSCs组小鼠β-Catenin含量显著升高; ALP和TRAP含量显著降低,BGP和E2含量显著升高; Osteopontin含量显著降低,Osterix,BMP-2,Runx2/Cbfa1和β-Catenin含量显著升高,差异有统计学意义(P<0.05)。与右归丸组相比,右归丸+BMSCs组β-Catenin含量显著升高,ALP和TRAP含量显著降低,BGP和E2含量显著升高; Osteopontin含量显著降低,Osterix,BMP-2,Runx2/Cbfa1和β-Catenin含量显著升高,差异有统计学意义(P<0.05)。结论:β-Catenin信号途径在骨质疏松性骨缺损骨愈合过程中具有重要的作用。右归丸是通过激活β-Catenin促进BMSCs成骨分化,从而促进骨质疏松性骨缺损骨愈合。
Abstract:
Abstract Objective:To investigate the mechanism of Yougui pill mediated β-catenin signaling pathway in the treatment of osteoporotic bone defect induced by BMSCs. Methods:Mouse bone marrow mesenchymal stem cells were isolated and expanded in vitro to identify their biological activity. Six-month-old mice were selected to establish an osteoporotic bone defect model and treated with Yougui pill[18.9 g/(kg·d)] for 6 weeks. Serum alkaline phosphatase(ALP),tartrate-resistant acid phosphatase(TRAP),serum osteocalcin(BGP)and estradiol(E2)were detected by enzyme-linked immunosorbent assay(Elisa). The expression of β-catenin was detected by immunohistochemistry. The expression of osteocalcin,osteopontin,osterix,osteoclast-specific transcription factor(Runx2/Cbfa1)and β-catenin mRNA were detected by real-time PCR. Results:Compared with the model group,the content of β-catenin in two group was significantly increased,and ALP and tartrate-resistant acid phosphatase(TRAP)were significantly decreased,while serum BGP and E2 levels were significantly increased. The contents of osteopontin,osterix,BMP-2,Runx2/Cbfa1 and β-catenin were significantly decreased,with statistical significance(P<0.05). Compared with Yougui pill group,the content of β-Catenin in Yougui pill and BMSCs group was significantly increased,the contents of ALP and TRAP were significantly decreased,and BGP and E2 contents were significantly increased. The contents of osteopontin,osterix,BMP-2,Runx2/Cbfa1 and β-catenin were significantly decreased,with statistical significance(P<0.05). Conclusion:The β-catenin signaling pathway plays an important role in the process of osteogenesis of osteoporotic bone defects. Yougui pill promotes osteogenic differentiation of BMSCs by activating β-catenin and promotes bone healing of osteoporotic bone defects.

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备注/Memo

备注/Memo:
基金项目:浙江省杭州市卫生计生科技计划项目(2017B21)
更新日期/Last Update: 2018-07-02