[1]展嘉文 朱立国△ 王尚全 冯敏山 尹训璐 韩涛.血管内皮生长因子及其受体在离体培养椎体终板退变过程中的表达[J].中国中医骨伤科杂志,2018,26(07):7-10.
　ZHAN Jiawen ZHU Liguo WANG Shangquan FENG Minshan YIN Xunlu HAN Tao.Expression of Vascular Endothelial Growth Factor and Its Receptor During Degeneration of Endplates in Vitro[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2018,26(07):7-10.
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血管内皮生长因子及其受体在离体培养椎体终板退变过程中的表达()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第26卷
期数:
2018年07期
页码:
7-10
栏目:
实验研究
出版日期:
2018-07-02

文章信息/Info

Title:
Expression of Vascular Endothelial Growth Factor and Its Receptor During Degeneration of Endplates in Vitro
文章编号:
1005-0205(2018)07-0007-04
作者:
展嘉文1; 2 朱立国1; 2△ 王尚全1 冯敏山1; 2 尹训璐1 韩涛1
1中国中医科学院望京医院(北京,100102) 2中医正骨技术北京市重点实验室
Author(s):
ZHAN Jiawen1; 2 ZHU Liguo1; 2△ WANG Shangquan1 FENG Minshan1; 2 YIN Xunlu1 HAN Tao1
1Wangjing Hospital of Chinese Academy of Chinese Medicine,Beijing 100102,China; 2Beijing Muniapal Key Laboratory of Traditional Chinese Mediaine Bone-setting Techuology,Beijing 100102,China.
关键词:
椎体终板;  血管内皮生长因子;  受体;  持续压力;  椎间盘退变
Keywords:
Keywords: vertebral endplate;  vascular endothelial growth factor;  receptor;  constant compressive load;  vertebral degeneration
分类号:
R-33
文献标志码:
A
摘要:
目的:研究持续压力对椎体终板内血管内皮生长因子(VEGF)及其受体(VEGFR)表达的影响,进一步探讨椎体终板退变的发生机制。方法:将16只新西兰白兔处死后在无菌条件下取出脊柱运动节段,随机分为对照组与压力组,均放入离体加载和培养装置中进行培养,其中压力组予持续29.4 N压力,对照组不予压力,于培养前及培养后第3,7及14天,两组各取8个标本,对其椎体终板进行苏木精-伊红染色,应用ELISA与实时PCR检测VEGF及VEGFR的表达。结果:椎体终板形态完整性在持续压力条件下逐渐被破坏; VEGF蛋白浓度在新鲜标本终板中为(450.74±9.74)ng/L,持续压力导致VEGF蛋白浓度降低为(327.57±6.00)ng/L,与对照组(392.88±2.13)ng/L相比,差异有统计学意义(P<0.05)。VEGFR蛋白浓度在新鲜标本终板中为(11.02±2.31)ng/L,持续压力导致VEGF蛋白浓度显著降低为(8.11±2.17)ng/L,与对照组(10.02±1.53)ng/L相比,差异有统计学意义(P<0.05)。实时PCR检测显示持续压力导致VEGF基因相对表达量减少(0.842±0.002),与对照组(0.965±0.005)相比,差异有统计学意义(P<0.05)。结论:持续压力导致离体培养兔脊柱运动节段内椎体终板VEGF的表达减少,提示VEGF参与了椎间盘退变过程。
Abstract:
Abstract Objective:To study the effect of constant compressive load on the expression of vascular endothelial growth factor(VEGF)and its receptor(VEGFR)in the vertebral endplate,and further explore the mechanism of vertebral endplate degeneration. Methods:All 16 New Zealand white rabbits were sacrificed,and the spinal segments were removed under aseptic conditions and were randomly divided into the controls group and the loading group. The specimens were isolated and cultured in the culturing and loading devices,in which the loading group were treated with constant compressive load of 3kg,the controls without load. Tissue integrity,matrix synthesis,and the matrix gene expression profile were assessed after 3,7 and 14 days of culture and were compared with those of fresh tissues. Results:The results showed that the morphological integrity of the vertebral endplate was gradually destroyed under constant compressive load; The concentration of VEGF protein in fresh tissues was(450.74±9.74)ng/L,and the load resulted in a decrease in VEGF protein concentration(327.57±6.00)ng/L,which was significantly different from that in the controls(392.88±2.13)ng/L(P<0.05). The concentration of VEGFR protein was(11.02±2.31)ng/L in fresh,and the concentration of VEGF decreased significantly(8.11±2.17)ng/L after constant load,which was significantly different from that in controls(10.02±1.53)ng/L(P<0.05). RT-PCR detection showed that the relative expression of VEGF gene was reduced(0.842±0.002)by constant load,and the difference was significant with controls(0.965±0.005)(P<0.05). Conclusion:The constant compressive load leads to a decrease in the expression of VEGF in the vertebral endplate of the spinal motion segments in ex vivo culturing,indicating that VEGF is involved in the process of intervertebral disc degeneration.

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备注/Memo

备注/Memo:
基金项目:国家中医药管理局国家中医临床研究基地业务建 设科研专项课题(JDZX2015274) 北京地区中医骨科康复服务能力与技术平台规范 化建设项目(110019) 国家体育总局中医特色技术在体育运动中的应用 (HXKT2017001) △通信作者 E-mail:zhulg95@aliyun.com
更新日期/Last Update: 2018-07-02