[1]董洁,王丽娟,孙艳红,等.水蛭素对糖尿病足溃疡大鼠创面愈合及血管生成的影响[J].中国中医骨伤科杂志,2026,34(04):30-35.[doi:10.20085/j.cnki.issn1005-0205.260405]
　DONG Jie,WANG Lijuan,SUN Yanhong,et al.Effects of Hirudin on Wound Healing and Angiogenesis of Diabetic Foot Ulcer Rats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2026,34(04):30-35.[doi:10.20085/j.cnki.issn1005-0205.260405]
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水蛭素对糖尿病足溃疡大鼠创面愈合及血管生成的影响()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第34卷
期数:
2026年04期
页码:
30-35
栏目:
基础研究
出版日期:
2026-04-10

文章信息/Info

Title:
Effects of Hirudin on Wound Healing and Angiogenesis of Diabetic Foot Ulcer Rats
文章编号:
1005-0205(2026)04-0030-06
作者:
董洁1; 王丽娟1; 孙艳红1; 刘昊1△
1唐山职业技术学院附属医院(河北 唐山,063000)
Author(s):
DONG Jie1; WANG Lijuan1; SUN Yanhong1; LIU Hao1△
1Affiliated Hospital of Tangshan Vocational & Technical College,Tangshan 063000,Hebei China.
关键词:
水蛭素;  血管内皮生长因子;  缺刻基因1;  糖尿病足溃疡;  创面愈合;  血管生成
Keywords:
hirudin;  vascular endothelial growth factor;  Notch1;  diabetic foot ulcer;  wound healing;  angiogenesis
分类号:
R285.5
DOI:
10.20085/j.cnki.issn1005-0205.260405
文献标志码:
A
摘要:
目的:探讨水蛭素调控血管内皮生长因子(VEGF)/缺刻基因1(Notch1)通路对糖尿病足溃疡(DFU)大鼠创面愈合及血管生成的影响。方法:将糖尿病足溃疡大鼠随机分为对照组、模型组、水蛭素低/高剂量组、抑制剂组,每组各10只。测定各组大鼠创面愈合率; ELISA法检测血清中TNF-α、IL-8、IL-6的表达; HE染色检测大鼠创面组织病理变化; 免疫组化染色检测创面组织CD34表达和微血管密度(MVD); Western Blot法检测大鼠创面组织中VEGF、Notch1蛋白的表达。结果:对照组皮肤组织结构正常,无病理性改变; 模型组新生血管分布较少,可见大量炎性细胞浸润; 水蛭素低/高剂量组新生血管依次增多,炎性细胞浸润依次减少; 抑制剂组新生血管进一步减少,炎性细胞浸润进一步增多。模型组TNF-α、IL-8、IL-6高于对照组,创面愈合率、CD34阳性率、微血管密度、VEGF、Notch1蛋白表达低于对照组,差异有统计学意义(P<0.05); 水蛭素低/高剂量组TNF-α、IL-8、IL-6低于模型组,创面愈合率、CD34阳性率、微血管密度、VEGF、Notch1蛋白表达高于模型组,差异有统计学意义(P<0.05); 抑制剂组TNF-α、IL-8、IL-6高于水蛭素高剂量组,创面愈合率、CD34阳性率、微血管密度、VEGF、Notch1蛋白表达低于水蛭素高剂量组,差异有统计学意义(P<0.05)。结论:水蛭素可能通过激活VEGF/Notch1通路,促进糖尿病足溃疡大鼠的创面愈合及血管生成。
Abstract:
Objective:To investigate the effects of hirudin on wound healing and angiogenesis in diabetic foot ulcer(DFU)rats through regulation of the vascular endothelial growth factor(VEGF)/Notch1 pathway.Methods:The DFU rats were randomly divided into the following groups:control group,model group,low-dose hirudin group,high-dose hirudin group,and inhibitor group,with 10 rats in each group.The wound healing rate of rats in each group was measured.ELISA was performed to measure the expression levels of TNF-α,IL-8,and IL-6 in serum.HE staining was performed to assess pathological changes in rat wound tissue.Immunohistochemical staining was performed to detect CD34 expression and microvascular density(MVD)in wound tissue.Moreover,Western Blot was used to measure the expression levels of VEGF and Notch1 proteins in rat wound tissues.Results:The skin tissue structure of the control group was normal and showed no pathological changes.The distribution of new blood vessels in the model group was relatively sparse,and with a large amount of inflammatory cell infiltration. In the low-dose and high-dose thrombin groups,the number of new blood vessels increased successively,while the inflammatory cell infiltration decreased successively.The inhibitor group showed a further reduction in neovascularization and an increase in inflammatory cell infiltration.The model group had higher levels of TNF-α,IL-8,and IL-6,and lower wound healing rate,CD34 positivity rate,MVD,expression of VEGF and Notch1 than the control group(P<0.05).The low-dose hirudin and high-dose hirudin groups had lower TNF-α,IL-8,and IL-6,and higher wound healing rates,CD34 positivity rates,MVD,VEGF,and Notch1 than the model group(P<0.05).The inhibitor group had higher levels of TNF-α,IL-8,and IL-6,and lower wound healing rate,CD34 positivity rate,MVD,expression of VEGF and Notch1 compared to the high-dose hirudin group(P<0.05).Conclusion:Hirudin may promote wound healing and angiogenesis in DFU rats by activating the VEGF/Notch1 pathway.

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(收稿日期:2025-08-27)

备注/Memo

备注/Memo:
基金项目:河北省中医药类科学研究课题计划项目(2025521)
△通信作者 E-mail:pyzwd@163.com
更新日期/Last Update: 2026-04-15