[1]李小军△,於浩,顾锐.原花青素B2对地塞米松诱导骨髓间充质干细胞成骨分化的影响[J].中国中医骨伤科杂志,2026,34(04):1-6.[doi:10.20085/j.cnki.issn1005-0205.260401]
　LI Xiaojun,YU Hao,GU Rui.Procyanidin B2 on the Mechanism of Osteogenic Differentiation of Dexamethasone-Induced Bone Marrow Mesenchymal Stem Cells[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2026,34(04):1-6.[doi:10.20085/j.cnki.issn1005-0205.260401]
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原花青素B2对地塞米松诱导骨髓间充质干细胞成骨分化的影响()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第34卷
期数:
2026年04期
页码:
1-6
栏目:
基础研究
出版日期:
2026-04-10

文章信息/Info

Title:
Procyanidin B2 on the Mechanism of Osteogenic Differentiation of Dexamethasone-Induced Bone Marrow Mesenchymal Stem Cells
文章编号:
1005-0205(2026)04-0001-06
作者:
李小军1△; 於浩1; 顾锐1
1无锡市中医医院(江苏 无锡,214000)
Author(s):
LI Xiaojun1△; YU Hao1; GU Rui1
1Wuxi Hospital of Traditional Chinese Medicine,Wuxi 214000,Jiangsu China.
关键词:
原花青素B2;  Hippo/YAP通路;  地塞米松;  骨髓间充质干细胞;  成骨分化
Keywords:
procyanidin B2;  Hippo/YAP pathway;  dexamethasone;  bone marrow mesenchymal stem cells;  osteogenic differentiation
分类号:
R285.5
DOI:
10.20085/j.cnki.issn1005-0205.260401
文献标志码:
A
摘要:
目的:探究原花青素B2调节Hippo/Yes相关蛋白(YAP)通路对地塞米松诱导骨髓间充质干细胞(BMSCs)成骨分化的影响。方法:从大鼠体内分离骨髓间充质干细胞,经鉴定后体外培养,分为对照组、地塞米松组、地塞米松+低/中/高剂量原花青素B2组、地塞米松+高剂量原花青素B2+YAP激活剂PY-60(地塞米松+高剂量原花青素B2+PY-60)组。MTT法检测细胞活力; 碱性磷酸酶(ALP)检测试剂盒检测ALP浓度; 茜红素染色检测细胞矿化结节; Western Blot法检测细胞Runx2、OCN、Collagen Ⅰ和Hippo/YAP通路相关蛋白表达。结果:成功分离并完成骨髓间充质干细胞培养。与对照组比,地塞米松组矿化结节数量、OD值、ALP浓度、Runx2、OCN、Collagen Ⅰ蛋白表达降低,p-YAP/YAP比值升高,差异有统计学意义(P<0.05); 与地塞米松组比,地塞米松+低/中/高剂量原花青素B2组矿化结节数量、OD值、ALP浓度、Runx2、OCN、Collagen Ⅰ蛋白表达升高,p-YAP/YAP比值降低,差异有统计学意义(P<0.05); 与地塞米松+高剂量原花青素B2组比,地塞米松+高剂量原花青素B2+PY-60组矿化结节数量、OD值、ALP浓度、Runx2、OCN、Collagen Ⅰ蛋白表达降低,p-YAP/YAP比值升高,差异有统计学意义(P<0.05)。结论:原花青素B2通过抑制Hippo/YAP通路减轻地塞米松诱导的骨髓间充质干细胞成骨分化抑制。
Abstract:
Objective:To investigate the effect of procyanidin B2 on dexamethasone-induced osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)via adjusting the Hippo/Yes-associated protein(YAP)pathway.Methods:BMSCs cells were isolated from rats,identified and cultured in vitro.The cells were then divided into control group,dexamethasone group,dexamethasone+low,medium,and high dose procyanidin B2 groups,and dexamethasone+high dose procyanidin B2+YAP activator PY-60(dexamethasone+high dose procyanidin B2+PY-60)group.MTT assay was performed to detect cell viability.The ALP detection kit was used to measure ALP activity.Alizarin Red S staining was performed to visualize and quantify mineralized nodules in cells.Moreover,Western Blot was used to measure the expression levels of Runx2,OCN,Collagen Ⅰ,and Hippo/YAP pathway-related proteins in cells.Results:BMSCs were successfully isolated and cultured.Compared with the control group,the dexamethasone group showed a decreased number of mineralized nodules,OD value,ALP concentration,Runx2,OCN,and Collagen Ⅰ protein expression,while increased p-YAP/YAP ratio(P<0.05).Compared with the dexamethasone group,the dexamethasone+low,medium,and high dose procyanidin B2 groups showed increased number of mineralized nodules,OD value,ALP concentration,Runx2,OCN,and Collagen Ⅰ protein expression,while decreased p-YAP/YAP ratio(P<0.05).Compared with the dexamethasone+high dose procyanidin B2 group,the dexamethasone+high dose procyanidin B2+PY-60 group showed decreased number of mineralized nodules,OD value,ALP concentration,Runx2,OCN,and Collagen Ⅰ protein expression,while increased p-YAP/YAP ratio(P<0.05).Conclusion:Procyanidin B2 alleviates dexamethasone-induced inhibition of osteogenic differentiation in BMSCs by inhibiting the Hippo/YAP pathway.

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(收稿日期:2025-09-09)

备注/Memo

备注/Memo:
基金项目:2013-2014年度江苏省中医药局科技项目(LZ13148)
△通信作者 E-mail:jvfnr1@sina.com
更新日期/Last Update: 2026-04-15