[1]徐丽,李孝林,张朝驹,等.基于炎症小体信号通路探讨和营通气片对大鼠腰椎间盘突出症寒凝证的作用机制[J].中国中医骨伤科杂志,2025,33(04):21-26+32.[doi:10.20085/j.cnki.issn1005-0205.250405 ]
　XU Li,LI Xiaolin,ZHANG Chaoju,et al.Exploring the Mechanism of Action of Heyingtongqi Tablets on Cold Coagulation Syndrome of Lumbar Disc Herniation in Rats Based on Inflammasome Signaling Pathway[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2025,33(04):21-26+32.[doi:10.20085/j.cnki.issn1005-0205.250405 ]
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基于炎症小体信号通路探讨和营通气片对大鼠腰椎间盘突出症寒凝证的作用机制()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第33卷
期数:
2025年04期
页码:
21-26+32
栏目:
实验研究
出版日期:
2025-04-10

文章信息/Info

Title:
Exploring the Mechanism of Action of Heyingtongqi Tablets on Cold Coagulation Syndrome of Lumbar Disc Herniation in Rats Based on Inflammasome Signaling Pathway
文章编号:
1005-0205(2025)04-0021-06
作者:
徐丽1; 李孝林2; 张朝驹1; 何川1△
1荆州市中医药研究所/荆州市中医医院(湖北 荆州,434000); 2长江大学
Author(s):
XU Li1; LI Xiaolin2; ZHANG Chaoju1; HE Chuan1△
1Jingzhou Institute of Traditional Chinese Medicine(Jingzhou Hospital of Traditional Chinese Medicine),Jingzhou 434000,Hubei China; 2Yangtze University,Jingzhou 434000,Hubei China.
关键词:
炎症小体;  信号通路;  和营通气片;  寒邪;  腰椎间盘突出症;  细胞凋亡
Keywords:
inflammasome;  signal pathway;  Heyingtongqi tablet;  cold evil;  lumbar disc herniation;  apoptosis
分类号:
R285.5
DOI:
10.20085/j.cnki.issn1005-0205.250405
文献标志码:
A
摘要:
目的:探讨和营通气片对大鼠腰椎间盘突出症(LDH)寒凝证的影响,基于NLRP3/NF-κB炎症小体信号通路探讨其可能的作用机制。方法:将30只SD雄性大鼠进行腰椎间盘突出症造模,然后随机挑选24只进行寒冷刺激,随后分成对照组(LDH大鼠模型)、模型组(LDH寒凝证大鼠模型)、和营通气片低剂量组、和营通气片高剂量组及吲哚美辛组,每组6只,干预28 d。用神经功能评分评价寒邪对腰椎间盘突出症的影响,用ELISA法检测IL-1β和TNF-α的含量,用Western Blot法和实时PCR检测NLRP3、NF-κB、ASC、IL-1β、TNF-α蛋白和mRNA的表达水平,用流式细胞仪检测细胞凋亡情况。结果:寒邪促进腰椎间盘突出症的发展,与对照组比较,模型组髓核组织PLA2活性升高,痛阈降低,神经根组织中NLRP3、NF-κB、ASC、IL-1β、TNF-α 蛋白和mRNA表达水平均提高,细胞凋亡增加(P<0.05); 与模型组比较,吲哚美辛组及和营通气片组大鼠神经根组织中髓核组织PLA2活性降低,痛阈升高,NLRP3、NF-κB、ASC、IL-1β、TNF-α 蛋白和mRNA表达水平均降低,细胞凋亡减少(P<0.05)。结论:和营通气片可能通过抑制NLRP3/NF-κB相关表达,缓解腰椎间盘突出症寒凝证大鼠髓核炎症及细胞凋亡,提高大鼠痛阈,NLRP3/NF-κB信号调控通路是和营通气片对大鼠腰椎间盘突出症寒凝证的可能作用机制。
Abstract:
Objective:To explore the effect of Heyingtongqi tablets on cold coagulation syndrome in rats with lumbar disc herniation(LDH),and exploring its possible mechanism of action based on the NLRP3/NF-κB inflammasome signaling pathway.Methods:30 SD male rats were modeled for lumbar disc herniation,and 24 rats were randomly selected for cold stimulation.Then they were divided into control group(LDH rat model),model group(LDH rat model of cold coagulation syndrome),Heyingtongqi tablet low-dose group,Heyingtongqi tablet high-dose group and indomethacin group,with 6 animals in each group.The intervention lasted for 28 d.Neural function score was used to evaluate the effect of cold evil on LDH,ELISA was used to detect the contents of IL-1β and TNF-α,and Western Blot and real-time PCR were used to detect the expression levels of NLRP3,NF-κB,ASC,IL-1β and TNF-α protein and mRNA.The apoptosis was detected by flow cytometry.Results:Cold pathogens promoted the development of LDH.Compared with the control group,PLA2 activity in nucleus pulposus tissue was increased,pain threshold was decreased,NLRP3,NF-κB,ASC,IL-1β,TNF-α protein and mRNA expression levels in nerve root tissue were increased in the model group,and apoptosis was increased(P<0.05).The activity of PLA2 in nucleus pulposus decreased,the pain threshold increased,the expression levels of NLRP3,NF-κB,ASC,IL-1β,TNF-α protein and mRNA decreased,and the apoptosis decreased(P<0.05)in intomethacin group and Heyingtongqi tablet group.Conclusion:Heyingtongqi tablets may alleviate nucleus pulposus inflammation and cell apoptosis in LDH cold coagulation syndrome rats by inhibiting NLRP3/NF-κB related expression,and increase the pain threshold of rats.Therefore,the NLRP3/NF-κB signaling regulatory pathway is a potential mechanism of Heyingtongqi tablets in treating cold coagulation syndrome of lumbar disc herniation in rats.

参考文献/References:

[1] HASVIK E,HAUGEN A J,GRØVLE L.Pinprick and light touch are adequate to establish sensory dysfunction in patients with lumbar radicular pain and disc herniation[J].Clin Orthop Relat Res,2021,479(4):651-663.
[2] 李满意,刘红艳,陈传榜,等.腰痹的证治[J].风湿病与关节炎,2022,11(4):49-52.
[3] 张朝驹,徐昌伟,涂扬茂,等.和营通气片治疗不同证型腰椎间盘突出症200例[J].中国临床康复,2003(14):2119.
[4] 吴海波,钟前波,邓寒冰,等.夹脊电针抑制P2X4/NLRP3炎性小体通路对神经根型颈椎病大鼠的保护作用[J].中国老年学杂志,2024,44(18):4574-4580.
[5] 王柯,叶寒露.基于TLR4/MyD88/NF-κB信号通路探究结筋点刃针松解法对膝骨关节炎兔股四头肌生物力学行为和炎症反应的影响[J].中国老年学杂志,2024,44(17):4312-4316.
[6] KANEUCHI Y,SEKIGUCHI M,KAMEDA T,et al.Temporal and spatial changes of μ-opioid receptors in the brain,spinal cord and dorsal root ganglion in a rat lumbar disc herniation model[J].Spine(Phila Pa 1976),2019,44(2):85-95.
[7] 苏友贤,段嘉豪,杨少锋.杨少锋运用补肾活血法从六经辨治腰椎间盘突出症经验[J].湖南中医杂志,2024,40(1):66-68.
[8] 尹晨东,仇湘中.补肝健腰方对腰椎间盘突出大鼠血清代谢组学的影响研究[J].中国中医骨伤科杂志,2023,31(9):7-14.
[9] 彭光远,何晓辰,苏敬彬,等.王金榜教授平脉辨证治疗寒凝证型腰椎间盘突出症的经验[J].天津中医药,2021,38(9):1174-1177.
[10] 何川,张朝驹,徐昌伟,等.和营通气片治疗椎动脉型颈椎病气滞血瘀证的疗效观察[J].湖北中医杂志,2015,37(3):13-14.
[11] 张朝驹,李孝林.和营通气片治疗腰椎间盘突出症的实验研究[J].中医药学刊,2005,23(4):641-642.
[12] 袁一鸣,王艳,裴飞,等.NLRP3炎性小体与周围神经损伤关系的研究进展[J].华中科技大学学报(医学版),2023,52(5):735-741.
[13] 胡焓,冯丹,田佳玉,等.黄芩苷通过抑制TLR2/MyD88/NF-κB信号通路减轻带状疱疹后遗神经痛大鼠神经炎症[J].中国老年学杂志,2023,43(24):6073-6078.
[14] MANGAN M S J,OLHAVA E J,ROUSH W R,et al.Targeting the NLRP3 inflammasome in inflammatory diseases[J].Nat Rev Drug Discov,2018,17(8):588-606.
[15] CHEN J,CHEN Z J.PtdIns4P on dispersed trans-Golgi network mediates NLRP3 inflammasome activation[J].Nature,2018,564(7734):71-76.
[16] WU S,ZHOU Y,LIANG J,et al.Upregulation of NF-κB by USP24 aggravates ferroptosis in diabetic cardiomyopathy[J].Free Radic Biol Med,2024,210:352-366.

备注/Memo

备注/Memo:
基金项目:全国名老中医专家传承工作室建设项目(国中医药人教函〔2022〕75号) 湖北省中医药管理局中医药科研面上项目(ZY2023M051) △通信作者 E-mail:hc78960968@sina.com
更新日期/Last Update: 2025-04-10