[1]颜清华,齐浩宏,汤照明△.毛蕊异黄酮抑制成纤维样滑膜细胞迁移侵袭的机制研究[J].中国中医骨伤科杂志,2025,33(04):16-20.[doi:10.20085/j.cnki.issn1005-0205.250404 ]
　YAN Qinghua,QI Haohong,TANG Zhaoming.Research on the Mechanism of Calycosin Inhibiting the Migration and Invasion of Fibroblast-Like Synoviocytes[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2025,33(04):16-20.[doi:10.20085/j.cnki.issn1005-0205.250404 ]
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毛蕊异黄酮抑制成纤维样滑膜细胞迁移侵袭的机制研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第33卷
期数:
2025年04期
页码:
16-20
栏目:
实验研究
出版日期:
2025-04-10

文章信息/Info

Title:
Research on the Mechanism of Calycosin Inhibiting the Migration and Invasion of Fibroblast-Like Synoviocytes
文章编号:
1005-0205(2025)04-0016-05
作者:
颜清华1; 齐浩宏1; 汤照明1△
1株洲市中医伤科医院(湖南 株洲,412000)
Author(s):
YAN Qinghua1; QI Haohong1; TANG Zhaoming1△
1Zhuzhou Traditional Chinese Medicine Orthopedics Hospital,Zhuzhou 412000,Hunan China.
关键词:
毛蕊异黄酮;  成纤维样滑膜细胞;  信号通路;  迁移;  侵袭
Keywords:
calycosin;  fibroblast-like synoviocytes;  signaling pathway;  migration;  invasion
分类号:
R-33
DOI:
10.20085/j.cnki.issn1005-0205.250404
文献标志码:
A
摘要:
目的:探讨毛蕊异黄酮调节TLR9/MyD88信号通路对人类风湿关节炎(RA)成纤维样滑膜细胞(FLS细胞)迁移和侵袭的影响。方法:将类风湿关节炎成纤维样滑膜细胞分为对照组(正常培养细胞)、低剂量毛蕊异黄酮组(5 μmol/L)、中剂量毛蕊异黄酮组(10 μmol/L)、高剂量毛蕊异黄酮组(20 μmol/L)和CpG 1826(1 μg/mL)+高剂量毛蕊异黄酮(20 μmol/L)组。类风湿关节炎成纤维样滑膜细胞活性、凋亡、迁移、侵袭及MMP-2、MMP-9、TLR9、MyD88蛋白表达的检测分别用CCK-8法、流式细胞术、划痕实验、Transwell法及Western Blot法进行。结果:与对照组比较,低/中/高剂量毛蕊异黄酮组细胞光密度(OD)值、划痕愈合率、侵袭细胞数及MMP-2、MMP-9、TLR9、MyD88蛋白水平显著降低,差异有统计学意义(P<0.05),细胞凋亡率升高,差异有统计学意义(P<0.05); 与高剂量毛蕊异黄酮组比较,CpG 1826+高剂量毛蕊异黄酮组升高细胞OD值、划痕愈合率、侵袭细胞数及MMP-2、MMP-9、TLR9、MyD88蛋白水平,差异有统计学意义(P<0.05),降低细胞凋亡率,差异有统计学意义(P<0.05)。结论:毛蕊异黄酮可能通过抑制TLR9/MyD88信号通路,进而抑制类风湿关节炎成纤维样滑膜细胞的迁移和侵袭。
Abstract:
Objective:To investigate the impacts of Calycosin on the migration and invasion of human rheumatoid fibroblast-like synoviocytes(RA-FLS cells)by regulating the TLR9/MyD88 signaling pathway.Methods:RA-FLS cells were assigned into control group(normal cultured cells),low-dose Calycosin group(5 μmol/L),medium-dose Calycosin group(10 μmol/L),high-dose Calycosin group(20 μmol/L),and CpG 1826(1 μg/mL)+high-dose Calycosin group(20 μmol/L).The activity,apoptosis,migration,invasion of RA-FLS cells,and the expression of MMP-2,MMP-9,TLR9,and MyD88 proteins were detected using CCK-8 assay,flow cytometry,scratch assay,Transwell assay,and Western Blot,respectively.Results:Compared with the control group,the OD value,scratch healing rate,number of invasive cells,the protein levels of MMP-2,MMP-9,TLR9,and MyD88 were greatly reduced(P<0.05),and the apoptosis rate was increased(P<0.05)in the low-dose Calycosin group,medium-dose Calycosin group,and high-dose Calycosin group.Compared with the high-dose Calycosin group,the OD value,scratch healing rate,number of invasive cells,the protein levels of MMP-2,MMP-9,TLR9,and MyD88 were greatly increased(P<0.05),and the apoptosis rate was reduced(P<0.05)in CpG 1826+high-dose calycosin group.Conclusion:Calycosin can inhibit the migration and invasion of RA-FLS cells,and its mechanism may be related to the TLR9/MyD88 signaling pathway.

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备注/Memo

备注/Memo:
基金项目:湖南省中医药科研计划项目(D2022126) △通信作者 E-mail:171895916@qq.com
更新日期/Last Update: 2025-04-10