[1]杨佳凡,薛纯纯,赵福来,等.健腰强脊方对腰椎不稳诱导椎间盘退变小鼠模型的影响[J].中国中医骨伤科杂志,2025,33(04):1-6.[doi:10.20085/j.cnki.issn1005-0205.250401 ]
　YANG Jiafan,XUE Chunchun,ZHAO Fulai,et al.Effect of Jianyao Qiangji Formula on a Mouse Model of Lumbar Spine Instability-Induced Intervertebral Disc Degeneration[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2025,33(04):1-6.[doi:10.20085/j.cnki.issn1005-0205.250401 ]
点击复制

健腰强脊方对腰椎不稳诱导椎间盘退变小鼠模型的影响()
分享到：

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第33卷
期数:
2025年04期
页码:
1-6
栏目:
实验研究
出版日期:
2025-04-10

文章信息/Info

Title:
Effect of Jianyao Qiangji Formula on a Mouse Model of Lumbar Spine Instability-Induced Intervertebral Disc Degeneration
文章编号:
1005-0205(2025)04-0001-06
作者:
杨佳凡1; 2; 薛纯纯2; 赵福来2; 刘爽2; 薛永鹏1; 2; 杜文岚2; 李灵星2; 宋敏1; 施杞2; 李晓锋2△
1甘肃中医药大学(兰州,730000); 2上海中医药大学附属市中医医院
Author(s):
YANG Jiafan1; 2; XUE Chunchun2; ZHAO Fulai2; LIU Shuang2; XUE Yongpeng1; 2; DU Wenlan2; LI Lingxing2; SONG Min1; SHI Qi2; LI Xiaofeng2△
1Gansu University of Chinese Medicine,Lanzhou 730000,China; 2Shanghai Municipal Hospital of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200071,China.
关键词:
椎间盘退变;  健腰强脊方;  软骨终板;  软骨基质
Keywords:
intervertebral disc degeneration;  Jianyao Qiangji formula;  cartilaginous endplate;  cartilage matrix
分类号:
R-33
DOI:
10.20085/j.cnki.issn1005-0205.250401
文献标志码:
A
摘要:
目的:观察健腰强脊方对腰椎不稳诱导椎间盘退变小鼠模型的影响。方法:8周龄SPF级雄性C57BL/6小鼠30只,随机分为假手术组、模型组及健腰强脊方组,每组各10只。模型组小鼠行腰椎不稳(LSI)手术,健腰强脊方组在模型组的基础上给予健腰强脊方(生药2.94 g/mL)灌胃干预; 假手术组和模型组则给予0.9%生理盐水灌胃。干预8周后,获得各组小鼠腰椎组织,进行苏木精-伊红染色和番红O-固绿染色观察椎间盘形态,并进一步分析椎间盘高度和软骨终板钙化及缺损情况; 免疫组织化学染色分析软骨终板Col Ⅱ、Col Ⅹ及MMP-9的表达水平。结果:苏木精-伊红染色和番红O-固绿染色显示,腰椎不稳术后椎间盘高度显著下降(147±74)μm,软骨终板钙化面积明显增多,差异有统计学意义(P<0.01); 健腰强脊方干预后椎间盘高度显著增加(96±21)μm,软骨终板钙化面积明显减少,差异有统计学意义(P<0.05)。免疫组织化学染色分析结果显示,软骨终板Col Ⅱ表达显著升高,Col Ⅹ和MMP-9蛋白表达降低,差异有统计学意义(P<0.05)。结论:健腰强脊方可以改善腰椎不稳诱导椎间盘退变模型小鼠软骨终板钙化和细胞外基质降解,从而起到延缓椎间盘退变的效果。
Abstract:
Objective:To observe the effect of Jianyao Qiangji formula on a mouse model of lumbar spine instability(LSI)-induced intervertebral disc degeneration(IVDD).Methods:Thirty 8-week-old C57BL/6J male mice were randomly divided into the sham group,model group and Jianyao Qiangji formula group.Mice in model group and Jianyao Qiangji formula group were performed with LSI surgery.The Jianyao Qiangji formula group was given the Jianyao Qiangji formula(raw herb 2.94 g/mL)by gavage while the sham group and the model group were given 0.9% saline gavage.After 8 weeks of intervention,the lumbar spine tissues of mice in each group were collected.Hematoxylin-Eosin(HE)staining and Safranine O-Fast Green staining were performed to observe the morphology of the intervertebral discs,the height of the intervertebral discs,and the calcification defects of the cartilage endplates.Besides,the expression level of Col Ⅱ,Col Ⅹ,and MMP-9 was detected by immunohistochemical staining.Results:The histomorphological observation of HE and Safranine O-Fast Green staining showed that the height of intervertebral discs decreased significantly by(147±74)μm after LSI surgery(P<0.01),and the calcification area of cartilage endplate increased significantly(P<0.01).After the intervention of Jianyao Qiangji formula,the height of intervertebral discs increased significantly by(96±21)μm(P<0.05),and the calcification area of cartilage endplate was reduced(P<0.05).Immunohistochemical results showed that the expression of Col Ⅱ of cartilage endplate was significantly increased(P<0.001),and the expression of Col Ⅹ and MMP-9 protein was decreased(P<0.05).Conclusion:Jianyao Qiangji formula can ameliorate cartilage endplate calcification,inhibit extracellular matrix degradation in LSI-induced IVDD mice,and thus delay intervertebral disc degeneration.

参考文献/References:

[1] TAO S,SHEN Z,CHEN J,et al.Red light-mediated photoredox catalysis triggers nitric oxide release for treatment of cutibacterium acne induced intervertebral disc degeneration[J].ACS Nano,2022,16(12):20376-20388.
[2] MAHER C,UNDERWOOD M,BUCHBINDER R.Non-specific low back pain[J].Lancet,2017,389(10070):736-747.
[3] VIDEMAN T,BATTIE M C,GIBBONS L E,et al.A new quantitative measure of disc degeneration[J].Spine J,2017,17(5):746-753.
[4] JACKSON T,THOMAS S,STABILE V,et al.Prevalence of chronic pain in low-income and middle-income countries:a systematic review and meta-analysis[J].Lancet,2015,385(Suppl 2):S10.
[5] KIRNAZ S,CAPADONA C,WONG T,et al.Fundamentals of intervertebral disc degeneration[J].World Neurosurg,2022,157:264-273.
[6] NEIDLINGER-WILKE C,GALBUSERA F,PRATSINIS H,et al.Mechanical loading of the intervertebral disc:from the macroscopic to the cellular level[J].Eur Spine J,2014,23(Suppl 3):S333-S343.
[7] ZHU Q,GAO X,LEVENE H B,et al.Influences of nutrition supply and pathways on the degenerative patterns in human intervertebral disc[J].Spine(Phila Pa),2016,41(7):568-576.
[8] 曹旺烽,陈长贤.椎间盘软骨终板退变的研究进展[J].中国中医骨伤科杂志,2016,24(10):70-74.
[9] 施杞,王拥军,周重建,等.气血理论在延缓椎间盘退变过程的运用与发展[J].中国中医骨伤科杂志,2006,14(S2):190-192.
[10] 王拥军,梁倩倩,崔学军,等.调和气血法防治慢性筋骨病的应用与发展[J].上海中医药杂志,2017,51(6):1-4.
[11] 孙凯,魏戌,朱立国,等.壮腰通络方对腰椎间盘退行性病变大鼠病理变化及血清炎症因子表达的影响[J].中国中医药信息杂志,2022,29(7):93-98.
[12] 沈兴潮,夏炳江,韦金忠,等.五福饮对椎间盘退变大鼠模型血清及椎间盘炎性因子表达的影响[J].中国中医药科技,2020,27(4):525-528.
[13] 杨光露,郭杨,马勇,等.扶阳宣痹汤对大鼠椎间盘退变及基质金属蛋白酶表达的影响[J].中国中医骨伤科杂志,2021,29(4):1-7.
[14] 付方达,邵晶奕,童培建,等.脊柱轴线力学失稳诱导椎间盘退变小鼠模型的建立及评价[J].中国中医骨伤科杂志,2020,28(1):1-7.
[15] 黄继汉,黄晓晖,陈志扬,等.药理试验中动物间和动物与人体间的等效剂量换算[J].中国临床药理学与治疗学,2004(9):1069-1072.
[16] LIU S,SUN Y,DONG J,et al.A mouse model of lumbar spine instability[J].J Vis Exp,2021,170:61722
[17] MOON S M,YODER J H,WRIGHT A C,et al.Evaluation of intervertebral disc cartilaginous endplate structure using magnetic resonance imaging[J].Eur Spine J,2013,22(8):1820-1828.
[18] WANG W J,YU X H,WANG C,et al.MMPs and ADAMTSs in intervertebral disc degeneration[J].Clin Chim Acta,2015,448:238-246.
[19] LI X,YANG S,QIN L,et al.Type Ⅱ collagen-positive embryonic progenitors are the major contributors to spine and intervertebral disc development and repair[J].Stem Cells Transl Med,2021,10(10):1419-1432.
[20] LIU S,YANG S D,HUO X W,et al.17β-estradiol inhibits intervertebral disc degeneration by down-regulating MMP-3 and MMP-13 and up-regulating type Ⅱ collagen in a rat model[J].Artif Cells Nanomed Biotechnol,2018,46(sup2):182-191.
[21] LAKSTINS K,YEATER T,ARNOLD L,et al.Investigating the role of culture conditions on hypertrophic differentiation in human cartilage endplate cells[J].J Orthop Res,2021,39(6):1204-1216.
[22] DU J,XU M,KONG F,et al.CB2R attenuates intervertebral disc degeneration by delaying nucleus pulposus cell senescence through AMPK/GSK3β pathway[J].Aging Dis,2022,13(2):552-567.
[23] LI L,WEI K,DING Y,et al.M2a macrophage-secreted CHI3L1 promotes extracellular matrix metabolic imbalances via activation of IL-13Rα2/MAPK pathway in rat intervertebral disc degeneration[J].Front Immunol,2021,12:666361.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(82374473,81973881) 上海医学创新发展基金会未来计划项目(WLJH2021ZY-GZS001,WLJH2021ZY-ZLZX001,WLJH2021ZY-MZY034,WL-XJRY-2021003K,WLJH2022ZY-ZYY001) 甘肃省中医药研究中心2022-2023年开放课题(zyzx-2023-14) △通信作者 E-mail:lixiaofeng0409@163.com
更新日期/Last Update: 2025-04-10