[1]刘敏,白爽,杨倩,等.臭氧水关节腔注射通过调节炎症和氧化应激促进大鼠膝关节软骨损伤后修复[J].中国中医骨伤科杂志,2024,32(04):13-17+23.[doi:10.20085/j.cnki.issn1005-0205.240403]
　LIU Min,BAI Shuang,YANG Qian,et al.Study of Injection of Ozone Water into Joint Cavity in Promoting the Repair of Knee Cartilage Injury in Rats by Regulating Inflammation and Oxidative Stress[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2024,32(04):13-17+23.[doi:10.20085/j.cnki.issn1005-0205.240403]
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臭氧水关节腔注射通过调节炎症和氧化应激促进大鼠膝关节软骨损伤后修复()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第32卷
期数:
2024年04期
页码:
13-17+23
栏目:
实验研究
出版日期:
2024-04-15

文章信息/Info

Title:
Study of Injection of Ozone Water into Joint Cavity in Promoting the Repair of Knee Cartilage Injury in Rats by Regulating Inflammation and Oxidative Stress
文章编号:
1005-0205(2024)04-0013-05
作者:
刘敏1; 白爽1; 杨倩1; 张汉孺1; 周友龙2△
1河南中医药大学针灸推拿学院(郑州,450046)
2河南中医药大学国际教育学院
Author(s):
LIU Min1; BAI Shuang1; YANG Qian1; ZHANG Hanru1; ZHOU Youlong2△
1College of Acupuncture and Massage,Henan University of Chinese Medicine,Zhengzhou 450046,China; 2College of International Education,Henan University of Chinese Medicine,Zhengzhou 450046,China.
关键词:
臭氧水;  膝骨关节炎;  软骨修复;  炎症;  氧化应激
Keywords:
ozone water;  knee osteoarthritis;  cartilage repair;  inflammation;  oxidative stress
分类号:
R-33
DOI:
10.20085/j.cnki.issn1005-0205.240403
文献标志码:
A
摘要:
目的:分析臭氧水关节腔注射治疗大鼠膝关节软骨损伤后修复的最佳浓度及干预周期,并探讨其潜在的作用机制。方法:210只SD大鼠采用碘乙酸钠制备膝骨关节炎(KOA)软骨损伤模型后,分别采用5,10,20 μg/mL的臭氧水,按照每1 d、3 d和7 d一次的治疗频率分别连续治疗1,2,4周。治疗结束后,测量各组大鼠膝关节直径,分析大鼠关节肿胀程度; 处死并充分暴露大鼠关节软骨面进行软骨表面大体评分,软骨组织苏木精-伊红(HE)染色及改良的Mankin's评分评价关节软骨的修复情况; ELISA法检测血清炎症因子(IL-1β、TNF-α和IL-10)水平; 比色法检测氧化应激(SOD和MDA)的水平。结果:当治疗周期为1周时,20 μg/mL臭氧水3 d治疗一次软骨修复效果最佳,差异有统计学意义(P<0.05),而当治疗周期持续2周或4周时,7 d治疗一次的软骨修复效果显著优于每3 d治疗一次,差异有统计学意义(P<0.05)。ELISA法检测结果显示20 μg/mL臭氧水关节腔注射,7 d治疗一次,治疗2周时可显著下调膝骨关节炎大鼠血清IL-1β和TNF-α的表达水平,上调IL-10的表达水平,且能显著提高膝骨关节炎大鼠SOD活性,降低MDA活性,差异有统计学意义(P<0.05)。结论:20 μg/mL的臭氧水每7 d一次,连续治疗2周时疗效最佳。此外,臭氧水关节腔注射可能通过调节炎症和氧化应激促进软骨组织修复,是膝骨关节炎治疗的有效候选方法。
Abstract:
Objective:To study the optimal concentration,treatment frequency and treatment cycle of injection of ozone water into joint cavity to repair knee osteoarthritis(KOA)rat cartilage,and to explore the potential mechanism.Methods:210 SD rats with KOA cartilage injury model were prepared by sodium iodoacetate.The rats were treated with ozonated water(5,10,20 μg/mL)for 1,2 and 4 weeks according to the treatment frequency of once every 1,3 and 7 d respectively.After treatment,the diameter of the knee joint in each group were measured.Rats were killed and the articular cartilage surface of the rats were fully exposed the articular cartilage surface for cartilage surface gross score.Hematoxylin-eosin(HE)staining and modified Mankin's score were used to evaluate the repair of articular cartilage.Serum levels of inflammatory factors(IL-1β,TNF-α,IL-10)were determined by ELISA.The colorimetric cartridge was used to detect the level of oxidative stress(SOD,MDA).Results:When the treatment cycle was 1 week,the treatment with 20 μg/mL ozone water once every 3 d had the best effect on cartilage repair(P<0.05).When the treatment cycle was 2 weeks and 4 weeks,the effect of cartilage repair once every 7 d was significantly better than once every 3 d(P<0.05).ELISA results showed that injection of 20 μg/mL ozone water into articular cavity once every 7 d,after 2 weeks' treatment,the expression level of pro-inflammatory cytokine of IL-1β and TNF-α significantly reduced.The expression level of anti-inflammatory cytokine of IL-10 increased.The SOD activity was significantly increased,and the MDA level in KOA rats was decreased(P<0.05).Conclusion:The 20 μg/mL ozone water is the optimal concentration,and once every 7 d for 2 weeks is the optimal treatment frequency and treatment cycle.Moreover,ozone water joint cavity injection may promote cartilage repair by regulating inflammation and oxidative stress,and is an effective candidate for KOA therapy.

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备注/Memo

备注/Memo:
基金项目:河南省特色骨干学科中医学学科建设项目(STG-ZYXKY-2020032,STG-ZYX02-202115)
△通信作者 E-mail:zhou_youlong@hactcm.edu.cn
更新日期/Last Update: 2024-04-15