[1]彭汉书,陈令斌,刘融,等.甘草甜素调控多发性骨髓瘤细胞增殖和凋亡机制研究[J].中国中医骨伤科杂志,2023,31(03):16-21.[doi:10.20085/j.cnki.issn1005-0205.230304]
　PENG Hanshu,CHEN Lingbin,LIU Rong,et al.Mechanism Study of Glycyrrhizin Regulating the Proliferation and Apoptosis of Multiple Myeloma Cells[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2023,31(03):16-21.[doi:10.20085/j.cnki.issn1005-0205.230304]
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甘草甜素调控多发性骨髓瘤细胞增殖和凋亡机制研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第31卷
期数:
2023年03期
页码:
16-21
栏目:
实验研究
出版日期:
2023-03-15

文章信息/Info

Title:
Mechanism Study of Glycyrrhizin Regulating the Proliferation and Apoptosis of Multiple Myeloma Cells
文章编号:
1005-0205(2023)03-0016-06
作者:
彭汉书1; 陈令斌2; 刘融2; 徐进3△
1武汉科技大学附属普仁医院手足显微外科(武汉,430080)
2武汉科技大学附属普仁医院骨科
3湖北文理学院附属医院(襄阳市中心医院)骨科
Author(s):
PENG Hanshu1; CHEN Lingbin2; LIU Rong2; XU Jin3△
1Department of Hand and Foot Microsurgery, Puren Hospital Affiliated to Wuhan University of Science and Technology, Wuhan 430080, China;
2Department of Orthopedics, Puren Hospital Affiliated to Wuhan University of Science and Technology, Wuhan 430080, China;
3Department of Orthopedics, Affiliated Hospital of Hubei University of Arts and Sciences(Xiangyang Central Hospital), Xiangyang 441021, Hubei China.
关键词:
甘草甜素;  微小核糖核酸-4651;  多发性骨髓瘤;  增殖;  凋亡
Keywords:
glycyrrhizin;  microRNA-4651;  multiple myeloma;  proliferation;  apoptosis
分类号:
R-33
DOI:
10.20085/j.cnki.issn1005-0205.230304
文献标志码:
A
摘要:
目的:探讨甘草甜素调控miR-4651表达对多发性骨髓瘤RPMI8226细胞增殖、凋亡的影响机制。方法:将多发性骨髓瘤RPMI8226细胞随机分为对照组、甘草甜素低、中、高浓度组、miR-4651组、miR-NC组、甘草甜素+anti-miR-4651组、甘草甜素+anti-miR-NC组; 用四甲基偶氮唑盐比色法(MTT)检测细胞增殖抑制率,流式细胞仪检测细胞凋亡,实时荧光定量聚合酶链式反应(RT-qPCR)检测miR-4651表达水平。结果:与对照组比较,甘草甜素低、中、高浓度组骨髓瘤RPMI8226细胞增殖抑制率、细胞凋亡率和miR-4651表达水平均逐渐升高(P<0.05),呈剂量依赖性。上调miR-4651表达后,骨髓瘤RPMI8226细胞增殖抑制率和细胞凋亡率均升高(P<0.05),而抑制miR-4651的表达则逆转了甘草甜素对骨髓瘤RPMI8226细胞增殖和凋亡的作用。结论:甘草甜素通过上调miR-4651表达可抑制多发性骨髓瘤RPMI8226细胞增殖,促进细胞凋亡。
Abstract:
Objective:To explore the mechanism of glycyrrhizin regulating the expression of miR-4651 on the proliferation and apoptosis of multiple myeloma RPMI8226 cells. Methods:Multiple myeloma RPMI8226 cells were randomly divided into control group, glycyrrhizin low, medium and high concentration group, miR-4651 group, miR-NC group, glycyrrhizin+anti-miR-4651 group and glycyrrhizin+anti-miR-NC group. Cell proliferation inhibition rate was detected by tetramethylazolium salt colorimetric method(MTT), apoptosis was detected by flow cytometry, real-time fluorescent quantitative PCR(RT-qPCR)was used to detect the expression level of miR-4651. Results:Compared with the control group, the proliferation inhibition rate, apoptosis rate and miR-4651 expression level of myeloma RPMI8226 cells in the low, medium and high concentration groups of glycyrrhizins were gradually increased in a dose-dependent manner(P<0.05). After up-regulating the expression of miR-4651, the proliferation inhibition rate and apoptosis rate of myeloma RPMI8226 cells were increased(P<0.05), while inhibiting the expression of miR-4651 reversed the efficacy of glycyrrhizin on the proliferation and apoptosis of myeloma RPMI8226 cells. Conclusion:Glycyrrhizin can inhibit the proliferation of multiple myeloma RPMI8226 cells and promote cell apoptosis by up-regulating the expression of miR-4651.

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备注/Memo

备注/Memo:
基金项目:湖北省卫健委科研基金项目(WJ2018H0042)
△通信作者 E-mail:516289565@qq.com
更新日期/Last Update: 2023-03-10