[1]李丰,周文华,陈大勇,等.迷迭香酸对大鼠急性脊髓损伤的神经保护作用及其机制[J].中国中医骨伤科杂志,2023,31(02):1-5.
　LI Feng ZHOU Wenhua CHEN Dayong LIU Chao FU Junchu PAN Dan TAN Zhihong TANG Shaolong HUANG Qinghua.Neuroprotective Effect of Rosmarinic Acid on Acute Spinal Cord Injury in Rats and Its Mechanism[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2023,31(02):1-5.
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迷迭香酸对大鼠急性脊髓损伤的神经保护作用及其机制()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第31卷
期数:
2023年02期
页码:
1-5
栏目:
实验研究
出版日期:
2023-02-15

文章信息/Info

Title:
Neuroprotective Effect of Rosmarinic Acid on Acute Spinal Cord Injury in Rats and Its Mechanism
文章编号:
1005-0205(2023)02-0001-05
作者:
李丰1; 周文华1; 陈大勇1; 刘超1; 付军初1; 潘丹1; 谭志红1; 唐少龙1; 黄庆华1△
1中南大学湘雅医学院附属株洲医院脊柱外科(湖南 株洲,412000)
Author(s):
LI Feng1 ZHOU Wenhua1 CHEN Dayong1 LIU Chao1 FU Junchu1 PAN Dan1 TAN Zhihong1 TANG Shaolong1 HUANG Qinghua1△
1Department of Spinal Surgery, Zhuzhou Hospital, Xiangya School of Medicine, Central South University, Zhuzhou 412000, Hunan China.
关键词:
迷迭香酸;  急性脊髓损伤;  神经保护;  氧化应激反应;  炎症反应
Keywords:
rosmarinic acid;  acute spinal cord injury;  neuroprotection;  oxidative stress;  inflammatory response
分类号:
R-33
文献标志码:
A
摘要:
目的:探究迷迭香酸(RA)对大鼠急性脊髓损伤(ASCI)的神经保护作用及其机制。方法:50只SD大鼠随机分为5组,即假手术组(Sham组)、急性脊髓损伤组、迷迭香酸低、中、高剂量组(RA-L、RA-M、RA-H组),除Sham组外均建立大鼠急性脊髓损伤模型,造模成功后,Sham组和急性脊髓损伤组灌胃等量生理盐水,而RA-L组、RA-M组、RA-H组灌胃5,20,40 mg/kg剂量迷迭香酸。在给药后第3,7,14,28天进行BBB评分; ELISA法检测脊髓损伤组织SOD、GSH-PX、MDA、TNF-α、IL-1β、IL-10、NO; Western Blot法检测脊髓损伤组织iNOS、BDNF、p-NF-κB、p-p38 MAPK蛋白表达。结果:与Sham组比较,急性脊髓损伤组造模后第3,7,14,28天后BBB评分降低; 与急性脊髓损伤组比较,给药第3,7,14,28天后,RA-L组、RA-M组、RA-H组BBB评分升高。与Sham组比较,急性脊髓损伤组SOD、GSH-PX活性降低,MDA含量、TNF-α、IL-1β、IL-10、NO含量增加,iNOS、BDNF、p-NF-κB、 p-p38 MAPK蛋白表达水平上调; 与急性脊髓损伤组比较,RA-L组、RA-M组、RA-H组SOD、GSH-PX活性升高,MDA含量、TNF-α、IL-1β、IL-10、NO含量降低,iNOS、BDNF、p-NF-κB、p-p38 MAPK蛋白表达水平下调。结论:迷迭香酸可能通过抑制NF-κB、p38 MAPK信号通路减轻大鼠急性脊髓损伤组织氧化应激反应及炎症反应,对大鼠急性脊髓损伤的神经有保护作用。
Abstract:
Objective: To investigate the neuroprotective effect and mechanism of rosmarinic acid(RA)on acute spinal cord injury(ASCI)in rats. Methods:In the study, 50 SD rats were randomly divided into Sham operation group(Sham group), ASCI group, RA low, medium and high dose groups(RA-L, RA-M, RA-H group). Except for sham group, ASCI models were established in rats. After successful modeling, the Sham group and ASCI group were given the same amount of normal saline, while the RA-L group, RA-M group and RA-H group were given 5, 20, and 40 mg/kg RA. BBB score was performed on the day 3, day 7, day 14 and day 28 after administration. ELISA was used to detect SOD, GSH-PX, MDA, TNF-α, IL-1β, IL-10 and NO in spinal cord injury tissue. Western Blot was used to detect the protein expression of iNOS, BDNF, p-NF-κB and p-p38 MAPK in injured tissue. Results:Compared with the Sham group, the BBB score of the ASCI group decreased at day 3, day 7, day 14, and day 28 after administration. Compared with the ASCI group, the RA-L group, RA-M group, RA-H group BBB score increased at day 3, day 7, day 14, and day 28 after administration. Compared with the Sham group, the activities of SOD and GSH-PX in the ASCI group decreased, the contents of MDA, TNF-α, IL-1β, IL-10 and NO increased, and the protein expressions of iNOS, BDNF, p-NF-κB and p-p38 MAPK were up-regulated. Compared with the ASCI group, the activities of SOD and GSH-PX in the RA-L group, RA-M group and RA-H group were increased, and the contents of MDA, TNF-α, IL-1β, IL-10 and NO were decreased. The protein expression levels of BDNF, p-NF-κB and p-p38 MAPK were down-regulated.Conclusion:RA may reduce oxidative stress and inflammatory response in rat ASCI spinal cord injury tissues by inhibiting NF-κB and p38 MAPK signaling pathways, and has a protective effect on the nerves of rat ASCI.

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备注/Memo

备注/Memo:
△通信作者 E-mail:entra@163.com
更新日期/Last Update: 2023-02-10