[1]闫坤,陈华,张瑞坤,等.益骨汤对去卵巢骨质疏松大鼠核因子κB受体活化因子配体信号通路的影响[J].中国中医骨伤科杂志,2022,30(09):1-6.
　YAN Kun,CHEN Hua,ZHANG Ruikun,et al.Efficacy of Yigu Decoction on the Signal Pathway of Nuclear Factor κB Receptor Activator Ligand in Ovariectomized Osteoporosis Rats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2022,30(09):1-6.
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益骨汤对去卵巢骨质疏松大鼠核因子κB受体活化因子配体信号通路的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第30卷
期数:
2022年09期
页码:
1-6
栏目:
实验研究
出版日期:
2022-09-15

文章信息/Info

Title:
Efficacy of Yigu Decoction on the Signal Pathway of Nuclear Factor κB Receptor Activator Ligand in Ovariectomized Osteoporosis Rats
文章编号:
1005-0205(2022)09-0001-06
作者:
闫坤1; 陈华2; 张瑞坤1; 王佳鑫1; 安忠诚2; 姚新苗1; 3△
1浙江中医药大学第三临床医学院(杭州,310053)
2浙江中医药大学附属第二医院
3浙江中医药大学附属第三医院
Author(s):
YAN Kun1; CHEN Hua2; ZHANG Ruikun1; WANG Jiaxin1; AN Zhongcheng2; YAO Xinmiao1; 3△
1The Third Clinical Medical College of Zhejiang Chinese Medical University,Hangzhou 310053,China; 2The Second Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310005,China; 3The Third Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310005,China.
关键词:
骨质疏松;  益骨汤;  破骨细胞;  核因子κB受体活化因子配体;  骨保护素;  肿瘤坏死因子受体相关因子6
Keywords:
osteoporosis;  Yigu decoction;  osteoclast;  receptor activator of nuclear factor-κB ligand;  osteoprotegerin;  TNF receptor associated factor 6
分类号:
R-33
文献标志码:
A
摘要:
目的:观察益骨汤对去卵巢骨质疏松大鼠骨组织中核因子κB受体活化因子配体(RANKL)信号通路的影响,分析益骨汤治疗骨质疏松症的作用机制。方法:取12周龄SD雌性大鼠50只,随机分为正常组、假手术组、模型组、雌激素组和益骨汤组,治疗1次/d(12周后给药),持续12周。以双能X线骨密度仪测定大鼠股骨骨密度,经micro-CT扫描测定骨组织形态参数,免疫组化法和Western Blot法检测OPG、RANK和TRAF-6蛋白表达量,qPCR法检测相应基因的表达量。结果:与假手术组比较,模型组大鼠的骨密度明显降低,差异有统计学意义(P<0.01); 与模型组比较,雌激素组和益骨汤组骨密度均有所升高,差异有统计学意义(P<0.01)。与模型组比较,益骨汤组和雌激素组大鼠BV/TV升高,差异有统计学意义(P<0.05); Po(tot)下降,差异有统计学意义(P<0.05)。与雌激素组比较,益骨汤组TB.Sp降低,差异有统计学意义(P<0.05)。免疫组化结果:与模型组比较,正常组、假手术组、雌激素组和益骨汤组OPG蛋白的积分光密度(IOD)值均升高(P<0.01),TRAF-6蛋白的IOD值降低,差异有统计学意义(P<0.01,P<0.01,P<0.01,P<0.05)。Western Blot结果:与模型组比较,雌激素组和益骨汤组OPG蛋白的表达升高,差异有统计学意义(P<0.05,P<0.01); RANK、TRAF-6蛋白的表达均降低,差异有统计学意义(P<0.05)。qPCR结果:与模型组比较,雌激素组和益骨汤组OPG基因的表达升高,差异有统计学意义(P<0.05); RANK、TRAF-6基因的表达降低,差异有统计学意义(P<0.01,P<0.05)。结论:益骨汤可提高骨密度和骨微结构,其机制可能与调控RANKL通路干预破骨细胞分化相关。
Abstract:
Objective:To observe the efficacy of Yigu decoction(YGD)on the signal pathway of nuclear factor κB receptor activator ligand(RANKL)in bone tissue of ovariectomized osteoporosis rats,and analyze the mechanism of Yigu decoction in treating osteoporosis.Methods:Fifty 12-week-old SD female rats were randomly divided into normal group,sham operation group,model group,estrogen group and YGD group.Each group was treated once a day for 12 weeks.Bone mineral density of femur in rats was measured by dual-energy X-ray bone densitometer.The morphological parameters of bone tissue were measured by micro-CT scanning.Immunohistochemistry and Western Blot were used to detect the expression of OPG,RANK and TRAF-6 protein.The expression of corresponding genes was detected by qPCR method.Results:Compared with sham operation group,the bone mineral density(BMD)of model group rats was significantly lower(P<0.01).Compared with the model group,the BMD in estrogen group and YGD group was higher(P<0.01).Compared with the model group,BV/TV was higher(P<0.05)and Po(tot)was lower(P<0.05).Compared with estrogen group,TB.Sp in YGD group was lower(P<0.05).Immunohistochemical results showed that compared with the model group,the integrated optical density(IOD)value of OPG protein in normal group,sham operation group,estrogen group and YGD group was higher(P<0.01),while the IOD value of TRAF-6 protein was lower(P<0.01,P<0.01,P<0.01,P<0.05).Western Blot results showed that compared with the model group,the expression of OPG protein in estrogen group and YGD group was higher(P<0.05,P<0.01),and the expression of RANK and TRAF-6 protein was lower(P<0.05).qPCR results showed that compared with the model group,the expression of OPG gene in estrogen group and YGD group was higher(P<0.05),and the expression of RANK and TRAF-6 gene was lower(P<0.01,P<0.05).Conclusion:Yigu decoction can improve bone density and bone microstructure parameters,and its mechanism may be related to inhibition of RANKL signaling pathway.

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备注/Memo

备注/Memo:
基金项目:浙江省自然科学基金项目(LY17H270005)
全国名老中医药专家传承工作室建设项目(国中医药人教发〔2014〕20号)
浙江省国医名师建设项目(浙卫发〔2018〕70号)
△通信作者 E-mail:yxmtcm@aliyun.com
更新日期/Last Update: 2022-09-10