[1]帅波 马陈 沈霖△ 杨艳萍 卢俊杰 杨功旭 孙敏.补肾活血方对激素性股骨头缺血性坏死小鼠肾素-血管紧张素系统经典轴的影响[J].中国中医骨伤科杂志,2022,30(06):1-5.
　SHUAI Bo MA Chen SHEN Lin YANG Yanping LU Junjie YANG Gongxu SUN Min.Efficacy of Bushen Huoxue Recipe on ACE/AngⅡ/AT1R of RAS Classical Axis in Steroid Induced Ischemic Necrosis of Femoral Head in Mice[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2022,30(06):1-5.
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补肾活血方对激素性股骨头缺血性坏死小鼠肾素-血管紧张素系统经典轴的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第30卷
期数:
2022年06期
页码:
1-5
栏目:
实验研究
出版日期:
2022-06-15

文章信息/Info

Title:
Efficacy of Bushen Huoxue Recipe on ACE/AngⅡ/AT1R of RAS Classical Axis in Steroid Induced Ischemic Necrosis of Femoral Head in Mice
文章编号:
1005-0205(2022)06-0001-05
作者:
帅波1 马陈1 沈霖1△ 杨艳萍1 卢俊杰1 杨功旭2 孙敏2
1华中科技大学同济医学院附属协和医院中西医结合科(武汉,430022)2湖北省中医院骨科
Author(s):
SHUAI Bo1 MA Chen1 SHEN Lin1△ YANG Yanping1 LU Junjie1 YANG Gongxu2 SUN Min2
1Department of Integrated Traditional Chinese Medicine and Western Medicine,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China; 2Department of Orthopaedics,Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430074,China.
关键词:
激素性股骨头坏死;  补肾活血方;  维生素D受体 mRNA;  血管紧张素;  血管紧张素转换酶
Keywords:
steroid induced ischemic necrosis of femoral head;  Bushen Huoxue recipe;  VDR mRNA;  angiotensin;  angiotensin converting enzyme
分类号:
R-33
文献标志码:
A
摘要:
目的:初步探讨补肾活血方对激素性股骨头缺血性坏死(INFH)小鼠肾素-血管紧张素系统(RAS)经典轴血管紧张素转换酶(ACE)/血管紧张素Ⅱ(AngⅡ)/血管紧张素Ⅱ-1型受体(AT1R)的影响。方法:将60只SPF 级C57BL/6雄性小鼠随机分5组,即健康对照组、激素性INFH模型组、补肾活血方组、骨化三醇组及阿利吉仑组。治疗3个月后,ELISA法检测循环血中1,25(OH)2D3及AngⅡ的含量,并检测股骨头局部VDR、ACE、AT1R mRNA表达情况,行免疫组化分析。结果:与模型组相比,健康对照组、补肾活血方组、骨化三醇组及阿利吉仑组循环血中1,25(OH)2D3含量明显升高,差异有统计学意义(P<0.05),而AngⅡ含量明显降低,差异有统计学意义(P<0.05)。与模型组相比,健康对照组、补肾活血方组、骨化三醇干预组及阿利吉仑组骨组织局部VDR mRNA的表达明显升高,差异有统计学意义(P<0.05),而ACE、AT1R表达明显降低,差异有统计学意义(P<0.05)。免疫组化结果显示:模型组骨组织中ACE及AT1R阳性表达较其他各组高,差异有统计学意义(P<0.05),而VDR阳性表达较其他各组低,差异有统计学意义(P<0.05)。结论:激素性INFH小鼠RAS经典轴ACE/AngⅡ/AT1R被激活,补肾活血方可激活1,25(OH)2D3/VDR信号通路,并能抑制激活的RAS经典轴ACE/AngⅡ/AT1R,这可能是补肾活血方防治激素性INFH的部分机制。
Abstract:
Objective:To study the efficacy of Bushen Huoxue recipe on ACE/AngⅡ/AT1R of steroid induced ischemic necrosis of femoral head(INFH)in mice.Methods:All 60 SPF C57BL/6 male mice were randomly divided into 5 groups:control group,steroid induced INFH model group,Bushen Huoxue recipe group,calcitriol intervention group and aliskiren intervention group.The levels of 1,25(OH)2D3 and AngⅡ in the circulating blood were detected by ELISA method; the expression of VDR,ACE,AT1R mRNA in the femoral head was detected,and immunohistochemistry was performed three months after treatment.Results:Compared with the model group,the content of 1,25(OH)2D3 in the circulating blood of the healthy control group,Bushen Huoxue recipe group,calcitriol intervention group and aliskiren group was increased significantly(P<0.05),while the content of AngⅡ was decreased significantly(P<0.05).Compared with the model group,the expression of VDR mRNA in bone tissue of healthy control group,Bushen Huoxue recipe group,calcitriol intervention group and aliskiren group was increased significantly(P<0.05),while the expression of ACE and AT1R was decreased significantly(P<0.05).The immunohistochemistry results were showed that the expression of ACE and AT1R in bone tissue of model group was higher than that of other groups(P<0.05),while the expression of VDR was lower than that of other groups(P<0.05).Conclusion:ACE/AngⅡ/AT1R of RAS classic axis in steroid induced INFH mice is activated.Bushen Huoxue recipe can activate 1,25(OH)2D3/VDR signal pathway and inhibit the activated ACE/AngⅡ/AT1R of RAS classic axis,which may be part of the mechanism of Bushen Huoxue recipe in the prevention and treatment of steroid induced INFH.

参考文献/References:

[1] SHUAI B,YANG Y,SHEN L,et al.Effects of Qing’e formula on the expression of bone metabolic markers and VDR mRNA in postmenopausal osteoporosis patients[J].Chin Med,2014,5(3):145-152.
[2] 帅波,沈霖,郭向飞,等.密骨胶囊对去卵巢骨质疏松大鼠体内25(OH)D3和1,25(OH)2D3含量及肾脏VDR mRNA表达的影响[J].中西医结合研究,2009,1(4):177-181.
[3] 帅波,沈霖,杨艳萍,等.补肾活血方治疗早期缺血性股骨头坏死患者的临床研究[J].中国中医骨伤科杂志,2013,21(1):11-14.
[4] SHUAI B,YANG Y,SHEN L,et al.Effect of Qing’e pill plus salvia on non-traumatic osteonecrosis of the femoral head of idiopathic type in earlier stage:a case report of a twelve-month of period[J].Chin Med,2014,5(2):113-117.
[5] SHEN L,MA C,SHUAI B,et al.Effects of 1,25 dihydroxyvitamin D3 on the local bone reninangiotensin system in a murine model of glucocorticoidinduced osteoporosis[J].Experimental and Therapeutic Medicine,2017,13(6):3297-3304.
[6] ABUOHASHISH H M,AHMED M M,SABRY D,et al.Angiotensin(1-7)ameliorates the structural and biochemical alterations of ovariectomy-induced osteoporosis in rats via activation of ACE-2/Mas receptor axis[J].Scientific Reports,2017,7(1):2293.
[7] METTA S,UPPALA S,BASALINGAPPA D R,et al.Association of angiotensin converting enzymegene insertion/deletion polymorphism with risk of ischemic heart disease in a population of smokers in southern India[J].Journal of Clinical & Diagnostic Research,2015,9(4):1-4.
[8] LI Y,CAO Y,ZENG Z,et al.Angiotensin-converting enzyme 2/angiotensin-(1-7)/Mas axis prevents lipopolysaccharide-induced apoptosis of pulmonary microvascular endothelial cells by inhibiting JNK/NF-κB pathways[J].Scientific Reports,2015,5(3):8209.
[9] LI Y,ZENG Z,CAO Y,et al.Angiotensin-converting enzyme 2 prevents lipopolysaccharide induced rat acute lung injury via suppressing the ERK1/2 and NF-κB signaling pathways[J].Scientific Reports,2016,6:27911.
[10] 李雄,袁浩,贝美莲,等.大量激素冲击应用与长期应用对股骨头坏死影响的动物实验[J].骨与关节损伤杂志,1999,14(4):242.
[11] 帅波,沈霖,杨艳萍,等.血管紧张素转换酶抑制剂对去卵巢骨质疏松小鼠骨组织结构的影响[J].中华骨质疏松和骨矿盐疾病杂志,2015,8(3):229-235.
[12] SHUAI B,YANG Y P,SHEN L,et al.Local renin-angiotensin system is associated with bone mineral density of glucocorticoid-induced osteoporosis patients[J].Osteoporosis International,2015,26(3):1063-1071.
[13] LI Y C,QIAO G,USKOKOVIC M,et al.Vitamin D:a negative endocrine regulator of the renin-angiotensin system and blood pressure[J].Journal of Steroid Biochemistry & Molecular Biology,2004,89/90:387-392.
[14] 鞠云枫,杨留才.1,25-(OH)2D3在调节RAS中的作用研究[J].中国医药导报,2009,6(36):21-22.
[15] 帅波,杨功旭,沈霖,等.加味青娥丸对激素性股骨头坏死小鼠局部1,25(OH)2D3/VDR mRNA/RAS信号转导通路的影响[J].中国中医骨伤科杂志,2017,25(7):1-5.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81974546,82174182,82004201)
中央高校基本科研业务费专项资金资助项目(2019kfyRCPY093)
△通信作者 E-mail:shenlinhb@sina.cn
帅波和马陈为共同第一作者
更新日期/Last Update: 1900-01-01