[1]王世海△ 武晔 王恒俊 张树茂.骨关节炎患者滑膜组织中胰岛素样生长因子结合蛋白2、表皮生长因子受体表达及临床意义[J].中国中医骨伤科杂志,2022,30(05):28-33.
　WANG Shihai WU Ye WANG Hengjun ZHANG Shumao.The Expression and Clinical Significance of Insulin-Like Growth Factor BindingProtein 2 and Epithelial Growth Factor Receptor in theSynovialTissue of Patients with Osteoarthritis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2022,30(05):28-33.
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骨关节炎患者滑膜组织中胰岛素样生长因子结合蛋白2、表皮生长因子受体表达及临床意义()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第30卷
期数:
2022年05期
页码:
28-33
栏目:
临床研究
出版日期:
2022-05-15

文章信息/Info

Title:
The Expression and Clinical Significance of Insulin-Like Growth Factor BindingProtein 2 and Epithelial Growth Factor Receptor in theSynovialTissue of Patients with Osteoarthritis
文章编号:
1005-0205(2022)05-0028-06
作者:
王世海1△ 武晔1 王恒俊2 张树茂3
1河北省沧州中西医结合医院创伤骨四科(河北 沧州,061000)
2河北省沧州中西医结合医院骨科
3河北省沧州中西医结合医院病理科
Author(s):
WANG Shihai1△ WU Ye1 WANG Hengjun2 ZHANG Shumao3
1Department of Traumatology and Orthopedics, Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Cangzhou 061000, Hebei China; 2Department of Orthopedics, Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Cangzhou 061000, Hebei China; 2Department of Pathology, Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Cangzhou 061000, Hebei China.
关键词:
膝骨关节炎; 滑膜组织; 胰岛素样生长因子结合蛋白2; 表皮生长因子受体
Keywords:
knee osteoarthritis;  synovial tissue;  insulin-like growth factor binding protein 2;  epidermal growth factor receptor
分类号:
R684.3
文献标志码:
A
摘要:
目的:探讨膝骨关节炎患者滑膜组织中胰岛素样生长因子结合蛋白2(IGFBP2)和表皮生长因子受体(EGFR)表达水平及其临床意义。方法:选取2019年3月至2021年3月进行手术治疗的75例膝骨关节炎患者为研究组,在手术过程中获取患者的滑膜组织标本; 另选取同期进行治疗的78例膝关节韧带断裂、半月板损伤的患者为对照组,在手术过程中获取体内需要去除的损伤的游离滑膜组织标本进行研究。采用实时荧光定量PCR(qRT-PCR)法测定所有受试者滑膜组织中IGFBP2 mRNA和EGFR mRNA的表达水平; 采用免疫组织化学法测定患者滑膜组织中IGFBP2和EGFR蛋白的阳性率,分析膝骨关节炎患者滑膜组织中IGFBP2和EGFR的表达水平与患者临床病理特征间的关系; Spearman法分析膝骨关节炎患者滑膜组织中IGFBP2和EGFR的相关性。结果:两组患者性别、年龄、身体质量指数比较,差异无统计学意义(P>0.05)。研究组患者滑膜组织中IGFBP2 mRNA表达水平、蛋白阳性率显著高于对照组,EGFR mRNA表达水平、蛋白阳性率显著低于对照组,差异有统计学意义(P<0.05); 膝骨关节炎患者滑膜组织中IGFBP2和EGFR蛋白表达呈负相关性,差异有统计学意义(r=-0.357,P<0.05); 患者滑膜组织IGFBP2和EGFR表达与患者病程及疾病严重程度、体内炎症水平、软骨代谢水平有关,差异有统计学意义(P<0.05)。结论:膝骨关节炎患者滑膜组织中IGFBP2水平升高、EGFR水平下降,呈负相关性,IGFBP2和EGFR表达水平可以显著反映患者疾病严重程度,可为临床诊断提供参考依据。
Abstract:
To explore the expression levels of insulin-like growth factor binding protein 2(IGFBP2)and epidermal growth factor receptor(EGFR)in synovial tissues of patients with knee osteoarthritis and their clinical significance. Methods:75 patients with knee osteoarthritis who underwent surgical treatment during March 2019 to March 2021 were selected as the research group, and 78 patients with non-knee osteoarthritis who were treated during the same period were selected as the control group. The synovial tissue samples of the patients were obtained during the operation for research. Real-time fluorescence quantitative PCR(qRT-PCR)was used to determine the expression levels of IGFBP2 mRNA and EGFR mRNA in the synovial tissues of all subjects; immunohistochemical method was used to determine the negative and positive of IGFBP2 and EGFR proteins in the synovial tissue. The relationship between the expression levels of IGFBP2 and EGFR in the synovial tissue of patients with knee osteoarthritis and the clinicopathological characteristics of the patients were analyzed. Spearman method was used to analyze the correlation between IGFBP2 and EGFR in synovial tissue of patients with knee osteoarthritis. Results:There was no significant difference in gender, age and body mass index between the two groups(P>0.05). The IGFBP2 mRNA expression level and protein positive rate in the synovial tissue of the study group were significantly higher than those of the control group, and the EGFR mRNA expression level and protein positive rate were significantly lower than those of the control group(P<0.05); the expression of IGFBP2 and EGFR proteins in the synovial tissue of patients with osteoarthritis was negatively correlated(r=-0.357, P<0.05); the expression of IGFBP2 and EGFR in the synovial tissue of patients was related to the course of the disease, the severity of the disease, the level of inflammation in the body and the level of cartilage metabolism(P<0.05). Conclusion: The level of IGFBP2 in the synovial tissue of patients with knee osteoarthritis increases and the level of EGFR decreases, and there is a negative correlation. The levels of IGFBP2 and EGFR can obviously reflect the severity of disease of patients, and may provide a reference for clinical diagnosis.

参考文献/References:

[1] HE L,PAN Y,YU J,et al.Decursin alleviates the aggravation of osteoarthritis via inhibiting PI3K-Akt and NF-κB signal pathway[J].International Immunopharmacology,2021,97:107657.
[2] 钱敏,李宁,谢兴文,等.膝关节骨性关节炎的中西医结合治疗进展[J].中医临床研究,2021,13(15):54-56.
[3] ZHENG L,ZHANG Z,SHENG P,et al.The role of metabolism in chondrocyte dysfunction and the progression of osteoarthritis[J].Ageing Research Reviews,2020,66:101249.
[4] 李琳,李琪,张林忠.胰岛素样生长因子-1对骨代谢调节的研究进展[J].实用骨科杂志,2021,27(8):732-735.
[5] YANG M,ZHANG T,ZHANG Y,et al.MYLK4 promotes tumor progression through the activation of epidermal growth factor receptor signaling in osteosarcoma[J].Journal of Experimental & Clinical Cancer Research:CR,2021,40(1):166.
[6] 中国中西医结合学会骨伤科专业委员会.膝骨关节炎中西医结合诊疗指南[J].中华医学杂志,2018,98(45):3653-3658.
[7] 安琪儿,张帅,江文宇,等.促进老年退行性骨关节炎软骨内源性修复的化合物研究进展[J].现代生物医学进展,2019,19(2):383-388.
[8] ELSHEIKH M M A,EL-HAWA M A A,EL-SAADANY H M,et al.The effect of balance training in knee osteoarthritis[J].Journal of Advances in Medicine and Medical Research,2020.DOI:10.9734/jammr/2020/V32i243 0775.
[9] 赵立,吴宗辉,冉丽娟.肌骨超声联合X线检查在老年膝骨关节炎临床诊断中的应用价值[J].中国老年学杂志,2018,38(23):5746-5749.
[10] 邵佳乐,李志忠,周建,等.低频脉冲电磁场通过IGF-1R/NO信号通路促进大鼠颅骨成骨细胞成熟及矿化[J].浙江大学学报(医学版),2019,48(2):158-164.
[11] 邵婉珍,王森,郭雄.胰岛素样生长因子结合蛋白-2在骨软骨疾病发生发展中的作用[J].国外医学(医学地理分册),2017,38(3):302-304.
[12] LI K,TANG Y,LI J,et al.Inhibition of hepatocyte carcinoma cell activity by pterostilbene via down-regulating insulin-like growth factor binding protein-2[J].Internation- al Journal of Clinical and Experimental Medicine,2017,10(9):13152-13158.
[13] ZHANG H,GE J,LU X.CircFADS2 is downregulated in osteoarthritis and suppresses LPS-induced apoptosis of chondrocytes by regulating miR-195-5p methylation[J].Archives of Gerontology and Geriatrics,2021,96:104477.
[14] 陈明,杨波,常彦海,等.大骨节病患者踝关节软骨IGF-1、IGFBP2基因表达的特征及意义[J].中华地方病学杂志,2021,40(3):179-185.
[15] 曾文波,兰生辉,蔡贤华,等.EGFR信号通路在骨性关节炎研究中进展[J].中华老年骨科与康复电子杂志,2019,5(2):109-113.
[16] PARK S,LEE S Y,KIM D,et al.Comparison of epidermal growth factor receptor tyrosine kinase inhibitors for patients with lung adenocarcinoma harboring different epidermal growth factor receptor mutation types[J].BMC Cancer,2021,21(1):52.
[17] 王华松,兰生辉,庞炯宇,等.抑制EGFR信号通路对骨折愈合早期骨内、外膜来源干细胞增殖、分化能力影响的研究[J].生物骨科材料与临床研究,2020,17(6):5-8.
[18] 戴燚,李浩,范彦博.青娥方调节MMP2/EGFR通路影响成骨细胞凋亡的实验研究[J].中国中医骨伤科杂志,2018,26(11):7-12.

备注/Memo

备注/Memo:
基金项目:沧州市重点研发计划指导项目(204106028)
△通信作者 E-mail:wsh1988w@163.com
更新日期/Last Update: 1900-01-01