[1]杜伟斌△ 华爱兰 沈福祥 杨亚冬 陈荣良 王拓 曹国平.壳聚糖/角蛋白/虎杖苷生物支架的制备及其性能的研究[J].中国中医骨伤科杂志,2021,29(12):1-5.
　DU Weibin HUA Ailan SHEN Fuxiang YANG Yadong.Study of Preparation and Properties of Chitosan/Keratin/Polydatin Biological Scaffolds[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2021,29(12):1-5.
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壳聚糖/角蛋白/虎杖苷生物支架的制备及其性能的研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第29卷
期数:
2021年12期
页码:
1-5
栏目:
实验研究
出版日期:
2021-12-15

文章信息/Info

Title:
Study of Preparation and Properties of Chitosan/Keratin/Polydatin Biological Scaffolds
文章编号:
1005-0205(2021)12-0001-05
作者:
杜伟斌1△ 华爱兰1 沈福祥1 杨亚冬2 陈荣良1 王拓1 曹国平1
Author(s):
DU Weibin1△ HUA Ailan1 SHEN Fuxiang1 YANG Yadong2
1Jiangnan Hospital Affiliated to Zhejiang Chinese Medical University(Hangzhou Xiaoshan Traditional Chinese Medicine Hospital),Hangzhou 311200,China; 2Hangzhou Medical College(Zhejiang Academy of Medical Sciences),Hangzhou 310013,China.
关键词:
壳聚糖;  角蛋白;  虎杖苷;  支架材料;  医用敷料;  软组织损伤
Keywords:
chitosan;  keratin;  polydatin;  scaffold material;  medical dressings;  soft tissue injury
分类号:
R-3
文献标志码:
A
摘要:
目的:应用冷冻干燥技术制备壳聚糖/虎杖苷/角蛋白复合支架,研究其物理化学性能。方法:采用CCK-8法比较Vero、HeLa细胞增殖抑制率,选择虎杖苷最适工作浓度; 制备不同体积比壳聚糖/角蛋白基础生物支架,进行形态学观察后,选择适宜体积比基础生物支架; 制备壳聚糖/角蛋白/虎杖苷生物支架,通过扫描电镜、孔隙率、吸水率、膨胀率等测定,对比物理化学性能。结果:不超过200 μmol/L虎杖苷浓度对细胞增殖抑制相对较低。4:6和3:7体积比壳聚糖/角蛋白基础生物支架形态学更适宜。4:6壳聚糖/角蛋白/虎杖苷生物支架孔隙最多,差异有统计学意义(P<0.5),且孔与孔间连通良好,孔隙率也相对更稳定。支架去酸后,4:6组和3:7组吸水膨胀率,差异有统计学意义(P<0.5),且支架在水溶液中稳定。结论:4:6壳聚糖/角蛋白配比含虎杖苷(最终浓度不超过200 μmol/L)的生物支架物理化学性能最佳,可为创面修复、软组织退肿等医用敷料的应用提供新思路。
Abstract:
Objective:To prepare chitosan/polydatin/keratin composite scaffolds by freeze-drying technology and study their physical and chemical properties.Methods:CCK-8 method was used to detect and compare the proliferation inhibition rates of Vero and HeLa cells,and the optimal working concentration of polydatin was selected.The chitosan/keratin based biological scaffolds with different volume ratios were prepared.After morphological observation,the appropriate volume ratio of basic biological scaffolds was selected.The chitosan/keratin/polydatin biological scaffold was prepared and its physical and chemical properties were measured by scanning electron microscopy(SEM),porosity,water absorption and expansion rate.Results:The inhibition of cell proliferation was relatively low when the concentration of polydatin was less than 200 μmol/L.The 4:6 and 3:7 volumes ratio of morphology of chitosan/keratin based biological scaffolds were more suitable.The 4:6 chitosan/keratin/polydatin biological scaffold had the largest pore size(P<0.5).The pore-to-pore connectivity was good,and the porosity was relatively stable.After the stents were deacidified,the water absorption and swelling rates of the 4:6 and 3:7 groups were significantly different(P<0.5),and the stents were stable in aqueous solution.Conclusion:The biological scaffold with 4:6 chitosan/keratin ratio containing polydatin(the final concentration is no more than 200 μmol/L)has the best physical and chemical properties,which can provide a new idea for the application of wound repair,soft tissue swelling reduction and other medical dressings.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金青年项目(81904053)浙江省医药卫生科技计划项目(2020KY796,2020KY797)杭州市萧山区社会发展重大科技计划项目(11216,2019318)1浙江中医药大学附属江南医院(杭州萧山中医院)(杭州,311200)2杭州医学院(浙江省医学科学院)△通信作者 E-mail:dwbbdm@163.com
更新日期/Last Update: 1900-01-01