[1]鲍荣华△ 周虹 李旭云 孔令成.补肾健脾方干预大鼠成骨细胞增殖和凋亡的实验研究[J].中国中医骨伤科杂志,2021,29(10):9-12.
　BAO Ronghua ZHOU Hong LI Xuyun KONG Lingcheng.Efficacy of Tonifying Kidney and Spleen Compound onProliferation and Apoptosis of Rat Osteoblasts[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2021,29(10):9-12.
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补肾健脾方干预大鼠成骨细胞增殖和凋亡的实验研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第29卷
期数:
2021年10期
页码:
9-12
栏目:
实验研究
出版日期:
2021-10-15

文章信息/Info

Title:
Efficacy of Tonifying Kidney and Spleen Compound onProliferation and Apoptosis of Rat Osteoblasts
文章编号:
1005-0205(2021)10-0009-04
作者:
鲍荣华1△ 周虹1 李旭云1 孔令成1
1杭州市富阳中医骨伤医院(杭州,311400)
Author(s):
BAO Ronghua1△ ZHOU Hong1 LI Xuyun1 KONG Lingcheng1
1Fuyang Traditional Chinese Medicine Orthopedics Hospital, Hangzhou 311400, China.
关键词:
补肾健脾方;  成骨细胞;  细胞凋亡;  骨质疏松症
Keywords:
Tonifying Kidney and Spleen compound;  osteoblasts;  apoptosis;  osteoporosis
分类号:
R-33
文献标志码:
A
摘要:
目的:通过对补肾健脾方调控细胞凋亡途径干预大鼠成骨细胞增殖和凋亡的实验研究,探讨其治疗骨质疏松症的作用机制。方法:15只6个月龄SD雌性大鼠,随机分为补肾健脾方组、阿伦膦酸钠组、空白对照组(等体积生理盐水),每组5只,连续给药12 d,腹主动脉采血收集血清备用。细胞实验分为3组:空白对照组,补肾健脾方含药血清组和阿伦膦酸钠含药血清组。运用MTS测定成骨细胞增殖,实时荧光定量聚合酶链式反应法(qRT-PCR)测定Caspase-3、ERO1、Bax和Bcl-2 mRNA表达量。结果:MTS测定结果显示48 h和72 h补肾健脾方含药血清均对大鼠成骨细胞的增殖无明显的影响,差异无统计学意义(P>0.05)。qRT-PCR结果显示补肾健脾方含药血清相比于空白对照组能明显降低Caspase-3(P<0.01)和Bax(P<0.05)基因表达水平,提高Bcl-2 mRNA表达量,差异有统计学意义(P<0.01),但阿伦膦酸钠含药血清组Caspase-3和Bax基因下调更明显。结论:补肾健脾方含药血清能减少成骨细胞的凋亡,可能是通过Caspase-3/Bcl-2途径实现的。补肾健脾方对治疗骨质疏松症有一定的作用。
Abstract:
Objective:To observe the efficacy of Tonifying Kidney and Spleen compound on the proliferation and apoptosis of rat osteoblasts, and to explore its mechanism on treatment of osteoporosis. Methods:Fifteen 6-month-old female SD rats were randomly divided into three groups: the Tonifying Kidney and Spleen compound group, the Alendronate sodium group, and the blank control group(equal volume of normal saline), with 5 rats in each group. The rats were treated for 12 d continuously. Blood samples were collected from abdominal aorta and serum was collected for reserve. The cells were divided into three groups: blank control group, Tonifying Kidney and Spleen compound group and Alendronate sodium group. The proliferation of osteoblasts was measured by MTS, and the mRNA expression of Caspase-3, ERO1,Bcl2 associated x(Bax)and B-cell lymphama-2(Bcl-2)were determined by quantitative real-time polymerase chain reaction(qRT-PCR). Results:The MTS assay results showed that Tonifying Kidney and Spleen compound group had no significant efficacy on the proliferation of rat osteoblasts at 48 h and 72 h(P>0.05). Compared with the negative control group, the results of qRT-PCR displayed that Tonifying Kidney and Spleen compound significantly reduced the expression of Caspase-3(P<0.01)and Bax(P<0.05), and increased the expression of Bcl-2(P<0.01), but the expression of Caspase-3 and Bax in Alendronate sodium group were more obvious. Conclusion:The serum containing Tonifying Kidney and Spleen compound can reduce the apoptosis of osteoblasts, which may be achieved by Caspase-3/Bcl-2 pathway. It is suggested that Tonifying Kidney and Spleen compound may be a potential drug target for the treatment of osteoporosis.

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备注/Memo

备注/Memo:
基金项目:浙江省(杭州市)
科技计划项目(20181228Y149)
△通信作者 E-mail:13647516@qq.com
更新日期/Last Update: 1900-01-01