[1]王峰 刘国强 潘浩 季文辉△.黄连素通过基因调控对骨肉瘤细胞增殖、凋亡和侵袭的影响[J].中国中医骨伤科杂志,2021,29(05):16-20.
　WANG Feng LIU Guoqiang PAN Hao JI Wenhui.Effects of Berberine on the Proliferation,Apoptosis andInvasion of Osteosarcoma Cells through Gene Regulation[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2021,29(05):16-20.
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黄连素通过基因调控对骨肉瘤细胞增殖、凋亡和侵袭的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第29卷
期数:
2021年05期
页码:
16-20
栏目:
实验研究
出版日期:
2021-05-15

文章信息/Info

Title:
Effects of Berberine on the Proliferation,Apoptosis andInvasion of Osteosarcoma Cells through Gene Regulation
文章编号:
1005-0205(2021)05-0016-05
作者:
王峰1 刘国强1 潘浩1 季文辉1△
1河北沧州中西医结合医院骨关节外二科(河北 沧州,061000)
Author(s):
WANG Feng1 LIU Guoqiang1 PAN Hao1 JI Wenhui1△
1Department of Orthopedics and Joint Surgery,Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine,Cangzhou 061000,Hebei China.
关键词:
黄连素;  骨肉瘤;  增殖;  侵袭
Keywords:
berberine;  osteosarcoma;  proliferation;  invasion
分类号:
R738.1
文献标志码:
A
摘要:
目的:探究黄连素通过长链非编码RNA HNF1A-AS1调控miRNA-363对骨肉瘤细胞增殖、凋亡和侵袭的影响。 方法:将骨肉瘤细胞系U2OS分为对照组、黄连素组、黄连素+HNF1A-AS1组和HNF1A-AS1组。黄连素+HNF1A-AS1组和HNF1A-AS1组转染pcDNA-HNF1A-AS1质粒,黄连素组、黄连素+HNF1A-AS1组在培养基中加入终浓度为100 μmol/L的黄连素。分别用CCK-8、流式细胞仪和Transwell检测各组细胞增殖、凋亡和侵袭能力。通过双荧光素酶报告验证HNF1A-AS1与miRNA-363之间的靶向关系。 结果: 与对照组比较,黄连素组的HNF1A-AS1表达水平明显降低,miRNA-363表达水平显著升高,细胞增殖和侵袭能力显著降低,细胞凋亡率明显上升(P<0.05); HNF1A-AS1组细胞内HNF1A-AS1表达水平、细胞增殖和侵袭能力显著升高,细胞凋亡率和miRNA-363表达水平显著降低,差异有 统计学意义(P<0.05)。黄连素+HNF1A-AS1组细胞内HNF1A-AS1表达水平、细胞增殖和侵袭能力显著低于HNF1A-AS1组而高于黄连素组,细胞凋亡率和miRNA-363表达水平显著高于HNF1A-AS1组而低于黄连素组,差异有统计学意义(P<0.05)。双荧光素酶报告实验证实HNF1A-AS1可以直接靶向调控miRNA-363。结论:黄连素可以通过抑制HNF1A-AS1上调miRNA-363的表达,从而抑制OS细胞的增殖、侵袭和促进凋亡。
Abstract:
To explore the efficacy of berberine on cell proliferation,apoptosis and invasion of osteosarcoma(OS)cells by regulating miRNA-363 through LncRNA HNF1A-AS1.Methods:OS cell line U2OS was divided into NC group,Ber group,Ber+HNF1A-AS1 group and HNF1A-AS1 group.PcDNA-HNF1A-AS1 plasmid was transfected into Ber+HNF1A-AS1 group and HNF1A-AS1 group.Ber and Ber+HNF1A-AS1 groups were added into the medium with Ber with a final concentration of 100 μmol/L.CCK-8,flow cytometry and Transwell were used to detect the proliferation,apoptosis and invasion of each group.The targeted relationship between HNF1A-AS1 and miRNA-363 was verified by dual-luciferase report.Results:Compared with the NC group,the expression level of HNF1A-AS1 in Ber group was significantly decreased,the expression level of miRNA-363 was significantly increased,cell proliferation and invasion ability were significantly decreased,and cell apoptosis rate was significantly increased(P<0.05).In the HNF1A-AS1 group,HNF1A-AS1 expression level,cell proliferation and invasion ability were significantly increased,while cell apoptosis rate and miRNA-363 expression level were significantly decreased(P<0.05).In Ber+HNF1A-AS1 group,the HNF1A-AS1 expression level,cell proliferation and invasion ability of HNF1A-AS1 group were significantly lower than those of HNF1A-AS1 group and higher than those of Ber group.The cell apoptosis rate and expression level of miRNA-363 were significantly higher than those of HNF1A-AS1 group and lower than those of Ber group(P<0.05).Double luciferase reporting experiments confirmed that HNF1A-AS1 could directly target miRNA-363.Conclusion:Ber can inhibit the proliferation,invasion and promote apoptosis of OS cells by inhibiting HNF1A-AS1 to up-regulate the expression of miRNA-363.

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备注/Memo

备注/Memo:
基金项目:河北省中医药管理局科研计划项目(2015296)
△通信作者 E-mail:wangfeng5782@163.com
更新日期/Last Update: 2021-05-15