[1]周煜虎 段明明△ 曹强.续断醇提物对骨关节炎的治疗作用及相关分子机制的研究[J].中国中医骨伤科杂志,2020,28(07):1-5.
　ZHOU Yuhu DUAN Mingming CAO Qiang.Study on the Effects of Dipsacusasperoides Alcohol Extract on the Treatment of Osteoarthritis and Its Related Molecular Mechanism[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2020,28(07):1-5.
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续断醇提物对骨关节炎的治疗作用及相关分子机制的研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第28卷
期数:
2020年07期
页码:
1-5
栏目:
实验研究
出版日期:
2020-07-10

文章信息/Info

Title:
Study on the Effects of Dipsacusasperoides Alcohol Extract on the Treatment of Osteoarthritis and Its Related Molecular Mechanism
文章编号:
1005-0205(2020)07-0001-05
作者:
周煜虎1 段明明1△ 曹强1
1延安大学附属医院创伤骨科(陕西 延安,716000)
Author(s):
ZHOU Yuhu1 DUAN Mingming1△ CAO Qiang1
1Department of Orthopaedic Trauma,Affiliated Hospital of Yan'an University,Yan'an 716000,Shaanxi China.
关键词:
续断醇提物;  骨关节炎;  滑膜炎
Keywords:
dipsacusasperoides alcohol extract;  osteoarthritis;  synovitis
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨续断醇提物(RD)对骨关节炎(OA)的治疗作用及相关信号通路的影响。方法:将SD雄性大鼠(220～250 g)48只随机分为假手术组、模型组、RD-L,RD-M,RD-H及阳性对照组,采用右膝关节前交叉韧带切断术复制大鼠骨关节炎模型,评估OA大鼠的关节炎指数和机械性痛阈值; 采用苏木精-伊红和TUNEL染色检测OA大鼠滑膜组织的病理变化; 采用ELISA和RT-PCR法分别检测OA大鼠血清和滑膜组织中IL-6,IL-1β,TNF-α的含量和表达水平; 采用Western Blot法检测OA大鼠滑膜组织中Wnt/β-catenin信号分子蛋白的表达水平。结果:续断醇提物能够减小关节炎指数和增加机械性痛阈值,差异有统计学意义(P<0.05),同时缓解滑膜炎性细胞浸润和滑膜细胞凋亡,除此之外,还能够降低OA大鼠血清中IL-6,IL-1β,TNF-α的含量和下调滑膜组织中IL-6,IL-1β,TNF-α的表达水平,也能够下调滑膜组织中Wnt和β-catenin的蛋白表达,差异有统计学意义(P<0.05)。结论:续断醇提物对骨关节炎具有潜在的保护作用,同时抑制滑膜组织Wnt/β-catenin信号通路活化。
Abstract:
Objective:To explore the effect of dipsacusasperoides alcohol extract(RD)on osteoarthritis(OA)and its role on regulating Wnt/β-catenin signaling pathway. Methods:48 SD male rats(220-250 g)were randomly divided into sham operation group,model group,RD-L,RD-M,RD-H and positive control group. The rat model of osteoarthritis was reconstructed by right anterior cruciate ligament debridement.The arthritis index and mechanical pain threshold of OA rats were evaluated. The pathological changes of synovial tissue in OA rats were detected by HE and TUNEL staining. The levels and expression levels of IL-6,IL-1β and TNF-α in serum and synovial tissue of OA rats were detected by ELISA and RT-PCR. The Wnt/β-catenin signaling molecule protein expression level in synovial tissue of OA rats was detected by Western Blot. Results:RD can reduce the arthritis index and increase the mechanical pain threshold and alleviated the occurrence of synovitis and synovial cell apoptosis. It can reduce the levels of IL-6,IL-1β,TNF-α in serum and down-regulate the expression levels of IL-6,IL-1β and TNF-α in synovial tissue of OA rats(P<0.05),and also down-regulate protein expression of β-catenin and Wnt1 in synovial tissue(P<0.05). Conclusion:RD has potential protective effect on osteoarthritis,and this effect may be related to the inhibition of Wnt/β-catenin signaling pathway.

参考文献/References:

[1] 陈明,王丹娜,高福明,等. 曲安奈德、玻璃酸钠联合硫酸氨基葡萄糖治疗膝骨性关节炎疗效评价[J]. 中国卫生检验杂志,2016,26(12):1725-1728.
[2] 陈明,董启榕,黄群,等.0.5 Gy X线照射通过Wnt/β-Catenin信号通路促进成骨细胞分化[J]. 中华医学杂志,2017,97(23):1820-1825.
[3] 鲁花,于露,甄欢欢,等.人参皂苷Rg1通过抑制Wnt/β-catenin通路促进退变人腰椎间盘髓核细胞的生长及胞外基质合成[J]. 中国病理生理杂志,2018,34(4):705-710.
[4] FERNÁNDEZ-TORRES J,ZAMUDIO-CUEVAS Y,LPEZ-REYES A,et al. Gene-gene interactions of the Wnt/β-catenin signaling pathway in knee osteoarthritis[J]. Mol Biol Rep,2018,45(5):1089-1098.
[5] 王婉莹,彩云,孙丽艳.Wnt/β-catenin信号通路在骨关节炎和大骨节病发病中的作用及研究进展[J]. 中华地方病学杂志,2018,37(11):933-936.
[6] 李胜光. 中医治疗膝关节骨性关节炎疗效分析[J]. 中国民族民间医药,2010,19(7):102.
[7] 王祥杰,潘月兴,杜志仙,等.壮筋续骨汤对大鼠骨折骨痂中骨形态发生蛋白-7和神经肽Y表达的影响[J]. 中华中医药杂志,2013,28(8):214-216.
[8] 覃香,赵顺吕,傅婷,等.壮医药线点灸配合针灸治疗膝关节骨性关节炎60例[J]. 中国中医骨伤科杂志,2017,25(10):45-47.
[9] HUANG H,SKELLY J D,AYERS D C,et al. Age-dependent changes in the articular cartilage and subchondral bone of C57BL/6 mice after surgical destabilization of medial meniscus[J]. Sci Rep,2017,7:42294.
[10] MOBASHERI A,BAY-JENSEN A C,SPIL W E V,et al. Osteoarthritis year in review 2016:biomarkers(biochemical markers)[J]. Osteoarthritis Cartilage,2018,26(3):312-318.
[11] VAN DALEN S C,BLOM A B,SLÖETJES A W,et al. Interleukin-1 is not involved in synovial inflammation and cartilage destruction in collagenase-induced osteoarthritis[J]. Osteoarthritis Cartilage,2017,25(3):385-396.
[12] LIETMAN C,WU B,LECHNER S,et al. Inhibition of Wnt/β-catenin signaling ameliorates osteoarthritis in a murine model of experimental osteoarthritis[J]. Jci Insight,2018,3(3):e96308.
[13] RAN J,MA C,XU K,et al. Schisandrin B ameliorated chondrocytes inflammation and osteoarthritis via suppression of NF-κB and MAPK signal pathways[J]. Drug Des Devel Ther,2018,12:1195-1204.
[14] 周伟,吴进,林晓光,等. 活血膏对膝骨性关节炎大鼠关节软骨Wnt/β-catenin信号通路的影响[J]. 世界中医药,2018,13(6):1495-1498.
[15] MIYAMOTO K,OHKAWARA B,ITO M,et al. Fluoxetine ameliorates cartilage degradation in osteoarthritis by inhibiting Wnt/β-catenin signaling[J]. Plos One,2017,12(9):e0184388.
[16] DING Q H,YE C Y,CHEN E M,et al. Emodin ameliorates cartilage degradation in osteoarthritis by inhibiting NF-κB and Wnt/β-catenin signaling in-vitro and in-vivo[J]. Int Immunopharmacol,2018,61:222-230.

备注/Memo

备注/Memo:
(收稿日期:2019-11-15)基金项目:陕西省教育厅科学研究项目(17JK0876) △通信作者 E-mail:lstlst99@163.com
更新日期/Last Update: 2020-07-10