[1]鄢来军 颜浪辉 林庆宾 唐焕章△.通督活血汤对大鼠脊髓损伤后内质网应激信号表达的影响[J].中国中医骨伤科杂志,2020,28(05):1-4.
　YAN Laijun YAN Langhui LIN Qingbin TANG Huanzhang.Effects of Tongduhuoxue Decoction on Expression of EndoplasmicReticulum Stress Pathway of Rats with Spinal Cord Injury[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2020,28(05):1-4.
点击复制

通督活血汤对大鼠脊髓损伤后内质网应激信号表达的影响()
分享到：

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第28卷
期数:
2020年05期
页码:
1-4
栏目:
实验研究
出版日期:
2020-05-10

文章信息/Info

Title:
Effects of Tongduhuoxue Decoction on Expression of EndoplasmicReticulum Stress Pathway of Rats with Spinal Cord Injury
文章编号:
1005-0205(2020)05-0001-04
作者:
鄢来军1 颜浪辉1 林庆宾1 唐焕章2△
1福建中医药大学中医骨伤及运动康复教育部重点实验室(福州,350122)2中国人民解放军联勤保障部队第900医院
Author(s):
YAN Laijun1 YAN Langhui1 LIN Qingbin1 TANG Huanzhang2△
1Key Laboratory of the Ministry of Education of the People’s Republic of China,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China; 2900th Hospital of the Joint Logistics Support Force of PLA,Fuzhou 350025,China.
关键词:
通督活血汤;  脊髓损伤;  内质网应激;  凋亡
Keywords:
Tongduhuoxue decoction;  spinal cord injury;  endoplasmic reticulum stress;  apoptosis
分类号:
R-33
文献标志码:
A
摘要:
目的:观察通督活血汤对大鼠脊髓损伤(SCI)后肢体运动功能恢复与内质网应激p-PERK/ATF4/CHOP信号通路表达的影响。方法:56只SD大鼠随机分为假手术组、模型组、实验组,利用改良Allen打击法建立脊髓损伤动物模型,分别于术后1,3,7 d后采用BBB评分和苏木精-伊红(HE)染色观察大鼠后肢功能与脊髓组织形态变化,Western Blot检测p-PERK,ATF4,CHOP表达情况。结果:自干预第3天起,实验组行为学评分优于模型组,差异有统计学意义(P<0.05或P<0.01)。苏木精-伊红染色提示实验组神经细胞水肿较轻,神经细胞形态较好,空泡样改变较少,损伤神经细胞恢复中,部分神经细胞细胞核肿大,核仁消失。Western Blot检测结果示:假手术组p-PERK,ATF4,CHOP表达较低; 模型组和实验组p-PERK,ATF4和CHOP在第3天达到高峰; 与模型组比较,实验组各时间点p-PERK,ATF4,CHOP的表达显著减少,差异有统计学意义(P<0.05或P<0.01)。结论:通督活血汤治疗SCI的作用机制可能与该方调控SCI后p-PERK/ATF4/CHOP信号表达有关。
Abstract:
Objective:To observe the effects of Tongduhuoxue decoction on limb motor function recovery and expression of endoplasmic reticulum stress related p-PERK/ATF4/CHOP pathway after spinal cord injury(SCI)in rats.Methods: 56 SD rats were randomly divided into control group,model group and experimental group.The Allen method was used to establish the animal model.The hind limb function and spinal cord histomorphology were detected by BBB and HE staining at 1,3 and 7 d after intervention.The expressions of p-PERK,ATF4 and CHOP were detected by Western Blot.Results:The behavioral score of the experimental group was better than that of the model group after 3 d intervention(P<0.05 or P<0.01).HE staining revealed that the experimental group had less edema of nerve cells,better morphology of nerve cells and vacuolization with damaged nerve cells recovery,and some nerve cells showed enlarged nucleus and the nucleolus disappears.Western Blot revealed that the expression of p-PERK,ATF4 and CHOP in the sham operation group was lower.The model group and the experimental group p-PERK,ATF4 and CHOP reached the peak values on the 3rd day.Compared with the model group,the expression of p-PERK,ATF4,CHOP in experimental group were significantly reduced at each time point(P<0.05 or P<0.01).Conclusion:The mechanism of the treatment of SCI by Tongduhuoxue decoction may be related to the expression of p-PERK/ATF4/CHOP pathway after SCI regulation.

参考文献/References:

[1] 杨俊松,郝定均,刘团江,等.急性脊髓损伤的临床治疗进展[J].中国脊柱脊髓杂志,2018,28(4):368-373.
[2] SIDDALL P J,MIDDLETON J W.Spinal cord injury-induced pain:mechanisms and treatments[J].Pain Manag,2015,5(6):493-507.
[3] BARBARA-BATALLER E,MENDEZ-SUAREZ J L,ALEMAN-SANCHEZ C,et al.Change in the profile of traumatic spinal cord injury over 15 years in Spain[J].Scand J Trauma Resusc Emerg Med,2018,26(1):27.
[4] 杨枭雄,于前进,秦江,等.脊髓损伤住院患者1 027例流行病学分析[J].脊柱外科杂志,2016,14(5):301-305.
[5] SONG S,TAN J,MIAO Y,et al.Crosstalk of autophagy and apoptosis:involvement of the dual role of autophagy under ER stress[J].J Cell Physiol,2017,232(11):2977-2984.
[6] MCQUISTON A,DIEHL J A.Recent insights into PERK-dependent signaling from the stressed endoplasmic reticulum[J].F1000Res,2017,6:1897.
[7] WU H,NG B H,THIBAULT G.Endoplasmic reticulum stress response in yeast and humans[J].Bioscience Reports,2014,34(4):321-330.
[8] 梁平,周绍稳.通督活血汤治疗退行性腰椎管狭窄症112例[J].中国中医骨伤科杂志,2014,22(8):55-56.
[9] 孟祥琨.通督活血汤配合后路椎板减压螺钉置入治疗胸腰椎段骨折合并脊髓损伤疗效观察[J].现代中西医结合杂志,2017,26(19):2154-2156.
[10] 李强,汤耿民.通督活血汤治疗退行性腰椎管狭窄症[J].中国中医骨伤科杂志,1990,6(3):19-21.
[11] DUNHAM K A,FLOYD C L.Contusion models of spinal cord injury in rats[M]//Animal Models of Movement Disorders,Humana Press,2011:345-362.
[12] QIU J.China spinal cord injury network:changes from within[J].Lancet Neurol,2009,8(7):606-607.
[13] KUMAR R,LIM J,MEKARY R A,et al.Traumatic spinal injury:global epidemiology and worldwide volume[J].World Neurosurg,2018,113:e345-e363.
[14] ZHU G,LEE A S.Role of the unfolded protein response,GRP78 and GRP94 in organ homeostasis[J].J Cell Physiol,2015,230(7):1413-1420.
[15] PENAS C,GUZMAN M S,VERDU E,et al.Spinal cord injury induces endoplasmic reticulum stress with different cell-type dependent response[J].J Neurochem,2007,102(4):1242-1255.
[16] 齐英娜,吴鑫杰,王延雷,等.从督论治脊髓损伤的研究进展[J].时珍国医国药,2018,29(6):1425-1427.
[17] 刘江亭,李慧芬,崔伟亮,等.丹参研究述要[J].中医药学报,2017,45(6):127-130.
[18] 国家药典委员会.中华人民共和国药典[M].北京: 中国医药科技出版社,2015.
[19] 李强,李同生.通督活血汤治疗腰椎管狭窄症的临床及实验研究[J].中国中医骨伤科杂志,1991,7(4):18-20.
[20] 范筱,张俐.益气活血法调控脊髓损伤后相关基因的表达[J].中华中医药杂志,2017,32(3):1168-1171.
[21] DOU H C,CHEN J Y,RAN T F,et al.Panax quinquefolius saponin inhibits endoplasmic reticulum stress-mediated apoptosis and neurite injury and improves functional recovery in a rat spinal cord injury model[J].Biomed Pharmacother,2018,102:212-220.
[22] LIU X,YANG J,LI Z,et al.Hyperbaric oxygen treatment protects against spinal cord injury by inhibiting endoplasmic reticulum stress in rats[J].Spine(Phila Pa 1976),2015,40(24):E1276-E1283.
[23] CHEN L,LIU L,XIE Z Y,et al.Protein kinase RNA-like ER kinase/eukaryotic translation initiation factor 2alpha pathway attenuates tumor necrosis factor alpha-induced apoptosis in nucleus pulposus cells by activating autophagy[J].J Cell Physiol,2019,234(7):11631-11645.

备注/Memo

备注/Memo:
基金项目:南京军区联勤部卫生部重点项目(14ZX26)福建省自然科学基金(2018J01891)△通信作者 E-mail:tanghz@126.com(收稿日期:2019-11-11)
更新日期/Last Update: 2020-05-10