[1]孟祥东 冯宇△ 李福龙 郭亚.盘龙七片对膝骨关节炎大鼠软骨细胞凋亡的抑制及其作用机理[J].中国中医骨伤科杂志,2020,28(02):1-4.
　MENG Xiangdong FENG Yu LI Fulong GUO Ya.Inhibitory Effect of Panlongqi Tablet on Chondrocyte Apoptosis inRats with Knee Osteoarthritis and Its Mechanism[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2020,28(02):1-4.
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盘龙七片对膝骨关节炎大鼠软骨细胞凋亡的抑制及其作用机理()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第28卷
期数:
2020年02期
页码:
1-4
栏目:
实验研究
出版日期:
2020-02-10

文章信息/Info

Title:
Inhibitory Effect of Panlongqi Tablet on Chondrocyte Apoptosis inRats with Knee Osteoarthritis and Its Mechanism
文章编号:
1005-0205(2020)02-0001-04
作者:
孟祥东1 冯宇1△ 李福龙1 郭亚1
1山东菏泽市中医医院(山东 菏泽,273000)
Author(s):
MENG Xiangdong1 FENG Yu1△ LI Fulong1 GUO Ya1
1Heze Traditional Chinese Medicine Hospital,Heze 273000,Shandong China.
关键词:
盘龙七片;  膝骨关节炎;  软骨细胞;  细胞凋亡;  炎性因子
Keywords:
Panlongqi tablet;  knee osteoarthritis;  chondrocytes;  apoptosis;  inflammatory factors
分类号:
R-33
文献标志码:
A
摘要:
目的:分析中成药盘龙七片对膝骨关节炎大鼠软骨细胞凋亡的抑制作用,并探讨其作用机理。方法:采用膝关节穿刺注射胶原酶法制备膝骨关节炎大鼠模型,检测不同剂量盘龙七片处理后大鼠膝关节肿胀及外周血中炎性因子IL-1β和IL-6水平变化情况; 同时检测盘龙七片对炎症因子诱导的软骨细胞凋亡及Bcl-2和Caspase-3表达水平、GSK3β磷酸化水平的影响。结果:高、低剂量盘龙七片均可降低膝骨关节炎大鼠膝关节肿胀程度及炎症因子IL-1β和IL-6水平。盘龙七片处理后,大鼠膝关节软骨组织中及体外炎症因子诱导的软骨细胞中凋亡抑制因子Bcl-2的表达水平明显增高,Caspase-3表达量则显著降低,激酶GSK3β磷酸化水平及细胞凋亡水平明显下降,差异有统计学意义(P<0.05)。以GSK3β激活剂LY294002作用于细胞后,可明显减弱盘龙七片对软骨细胞凋亡的抑制作用。结论:盘龙七片可通过抑制GSK3β激酶活性,缓解膝关节软骨细胞的凋亡,进而对膝骨关节炎发挥治疗作用。
Abstract:
Objective:To analysis the inhibitory effect of Panlongqi tablet on chondrocyte apoptosis in rats with knee osteoarthritis and its potential mechanism.Methods:Model rats of knee osteoarthritis were established by injection of collagenase into knee joint.The swelling of knee joint and the levels of IL-1β and IL-6 in peripheral blood of rats were tested after treatment with different doses of Panlongqi tablet.At the same time,the effects of Panlongqi tablet on chondrocyte apoptosis,Bcl-2 and Caspase-3 expression level,GSK3β kinase level induced by inflammatory factors were detected.Results:The degree of knee swelling and the levels of inflammatory factors IL-1β and IL-6 in rats with knee osteoarthritis were reduced by both high and low dose of Panlongqi tablet.Besides,the expression of Bcl-2 and Caspase-3 in articular cartilage and chondrocytes induced by inflammatory factors in vitro were significantly increased,whereas the levels of Caspase-3 and apoptosis as well as GSK3β kinase were significantly decreased(P<0.05).After treatment with GSK3β activator LY294002,the inhibitory effect of Panlongqi tablet on chondrocyte apoptosis was significantly weakened.Conclusion:Panlongqi tablet plays a role in the treatment of knee osteoarthritis by inhibiting the activity of GSK3β kinase,alleviating the apoptosis of chondrocytes in knee joint.

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备注/Memo

备注/Memo:
△通信作者 E-mail:fy654-2@163.com(收稿日期:2019-08-10)
更新日期/Last Update: 2020-02-10