[1]郑怀亮 郭晓辉△ 李艳侠 夏祖辉 李磊 倘艳锋.川芎嗪对脊髓缺血再灌注损伤自噬相关蛋白影响的实验研究[J].中国中医骨伤科杂志,2019,27(12):5-8.
　ZHENG Huailiang GUO Xiaohui LI Yanxia XIA Zuhui LI lei TANG Yanfeng.Experimental Study on the Effect of Ligustrazine on Spinal Cord Ischemia-reperfusion Injury Autophagy Correlative Protein[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2019,27(12):5-8.
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川芎嗪对脊髓缺血再灌注损伤自噬相关蛋白影响的实验研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第27卷
期数:
2019年12期
页码:
5-8
栏目:
实验研究
出版日期:
2019-12-04

文章信息/Info

Title:
Experimental Study on the Effect of Ligustrazine on Spinal Cord Ischemia-reperfusion Injury Autophagy Correlative Protein
文章编号:
1005-0205(2019)12-0005-04
作者:
郑怀亮1 郭晓辉1△ 李艳侠1 夏祖辉1 李磊1 倘艳锋1
1河南省洛阳正骨医院(河南省骨科医院)(河南 洛阳,471002)
Author(s):
ZHENG Huailiang1 GUO Xiaohui1△ LI Yanxia1 XIA Zuhui1 LI lei1 TANG Yanfeng1
1Henan Luoyang Orthopaedic Hospital(Henan Orthopaedic Hospital),Luoyang 471002,Henan China.
关键词:
川芎嗪;  自噬;  蛋白;  脊髓缺血再灌注损伤
Keywords:
ligustrazine;  autophagy;  protein;  spinal cord ischemia-reperfusion injury
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨川芎嗪对脊髓缺血再灌注自噬蛋白Beclinl,LC3及P62影响的机制。方法:48只SD大鼠随机分为假手术组、模型组、川芎嗪组和3-MA(自噬抑制剂)处理组,造模后,在缺血3 h及6 h后进行行为学评分并取材,免疫组化检测Beclinl,LC3及P62表达变化。结果:在缺血3 h及6 h后自噬相关蛋白Beclin1,LC3 和 P62等方面进行比较,模型组、川芎嗪组、3-MA组与假手术组差异有统计学意义(P<0.05),三种蛋白均表达上调,模型组尤其明显,透射电镜发现干预后显著抑制自噬现象,可以推测川芎嗪干预优于3-MA,且SCII后行为学评分也与其一致。结论:川芎嗪可能会对细胞自噬进行双向调节,保护神经细胞。
Abstract:
Objective:To investigate the mechanism of ligustrazine on autophagic protein Beclin-1,LC3 and P62 in spinal cord ischemia-reperfusion injury(SCII).Methods:Forty-eight SD rats were randomly divided into 4 groups with sham operation group,model group,ligustrazine group and 3-MA(autophagy inhibitor)treatment group.The spinal cord ischemia-reperfusion injury model was built.Behavioral scores and samples were collected after 3 h and 6 h of ischemia.The expressions of Beclin-1,LC3 and P62 were detected by immunohistochemistry.Results:The expressions of autophagy-related proteins Beclin-1,LC3 and P62 were compared after ischemia for 3 h and 6 h.There was significant difference among model group,ligustrazine group,3-MA group and sham operation group(P< 0.05).The expression of all three proteins were up-regulated,especially in model group.Transmission electron microscopy showed that 3-MA intervention significantly inhibited the occurrence of autophagy.We can speculated that ligustrazine intervention was better than 3-MA,and the behavioral score after SCII was consistent with it.Conclusion:Ligustrazine may regulate the autophagy in both two directions to protect nerve cells.

参考文献/References:

[1] WYNN M M,ACHER C W.A modern theory of spinal cord ischemia/injury in thoracoabdominal aortic surgery and its implications for prevention of paralysis[J].J Cardiothorac Vasc Anesth,2014,28(4):1088-1099. [2] 姜宇懋,王丹巧.川芎嗪药理作用研究进展[J].中国现代中药,2016,18(10):1364-1370. [3] ZIVIN J A,DEGIROLAMI U.Spinal coed infarction:a higllly reproducible stroke model[J].Strodk,1980,11(2):200-205. [4] LIU B,HUANG W,XIAO X,et al.Neuroprotective effect of ulinastatin on spinal cord ischemia-reperfusion injury in rabbits[J].Oxid Med Cell Longev,2015:624819. [5] 杨成喜,张蕾,吴风雷,等.瘦素、雌激素及雌激素受体在肺癌组织中的表达及其相关性[J].肿瘤防治研究,2013,40(6):572-575. [6] JU L L,ZHAO C Y,YE K F,et al.Expression and clinical implicalion of Beclinl,HMGBl,p62,survivin,BRCAl and ERCCl inepithelial ovarian tumor tissues[J].Eur Rev Med Pharmacol Sci,2016,20(10):1993-2003. [7] 张宏,张慧.多细胞生物自噬的分子机制和生理功能[J].安徽大学学报(自然科学版),2018,42(5):105-114. [8] 李建荣,蒋晓帆,张磊,等.脑缺血模型中皮层Sirt3与自噬蛋白LC3表达的相关性[J].中华神经外科疾病研究杂志,2018,17(5):387-390.[9] YANG R,SONG Z,WU S,et al.Toll-like receptor 4 contributes to a myofibroblast phenotype in cardiac fibroblasts and is assoeiated with autophagy after myocardial infarction in a mouse model[J].Atherosclerosis,2018,279:23-31. [10] JIANG W W,HUANG B S,HAN Y,et al.Sodium hydrosulfide attenuates cerebral isehemia/reperfusion injury by suppressing overactivated autophagy in rats[J].FEBS Open Bio,2017,7(11):1686-1695. [11] 苏朋,孙永明,华俊,等.大鼠脊髓损伤后细胞自噬的变化及高压氧对其影响[J].中华航海医学与高气压医学杂志,2016,23(1):31-35. [12] 孙晓伟,高营,王新宇,等.针刺对脑缺血再灌注损伤神经细胞自噬影响的研究概况[J].中医药信息,2019,36(2):122-126. [13] ERLICH S,ALEXANDROVICH A,SHOHAMI E,et al.Rapamycin is a neuroprotective treatment for traumatic brain injury[J].Neurobiol Dis,2007,26(1):86-93. [14] ZHANG Y B,LI S X,CHEN X P,et al.Autophagy is activated and might protect neurons from degeneration after traumatic brain injury[J].Neurosci Bull,2008,24(3):143-149. [15] KOMATSU M,WAGURI S,CHIBA T,et al.Loss of autophagy in the central nervous system causes neurodegeneration in mice[J].Nature,2006,441(7095):880-884. [16] LIU C L,CHEN S,DIETRICH D,et al.Changes in autophagy after traumatic brain injury[J].J Cereb Blood Flow Metab,2015,28(4):674-683. [17] KANNO H,OZAWA H,SEKIGUCHI A,et al.The role of autophagy in spinal cord injury[J].Autophagy,2013,5(3):390-392.

备注/Memo

备注/Memo:
基金项目:河南省中医药科学研究专项课题(2015ZY02067)△通信作者 E-mail:gxh2005055@126.com(收稿日期:2019-05-16)
更新日期/Last Update: 2019-12-04