[1]邹恩苗 高丽萍 胡洁 林海燕 屠文展△.督脉电针治疗对脊髓损伤大鼠神经细胞自噬及凋亡的影响[J].中国中医骨伤科杂志,2019,27(11):11-14.
　ZOU Enmiao GAO Liping HU Jie LIN Haiyan TU Wenzhan.Effect of Du Meridian Electro Acupuncture on Autophagy and Apoptosis after Spinal Cord Injury in Rats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2019,27(11):11-14.
点击复制

督脉电针治疗对脊髓损伤大鼠神经细胞自噬及凋亡的影响()
分享到：

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第27卷
期数:
2019年11期
页码:
11-14
栏目:
实验研究
出版日期:
2019-10-30

文章信息/Info

Title:
Effect of Du Meridian Electro Acupuncture on Autophagy and Apoptosis after Spinal Cord Injury in Rats
文章编号:
1005-0205(2019)11-0011-04
作者:
邹恩苗1 高丽萍1 胡洁1 林海燕1 屠文展1△
1温州医科大学附属第二医院(育英儿童医院) (浙江 温州,325027)
Author(s):
ZOU Enmiao1 GAO Liping1 HU Jie1 LIN Haiyan1 TU Wenzhan1△
1The Second Affiliated Hospital(Yuying Children's Hospital)of Wenzhou Medical University,Wenzhou 325027, Zhejiang China.
关键词:
督脉电针;  脊髓损伤;  细胞凋亡;  自噬;  微管相关蛋白1轻链3-Ⅱ
Keywords:
Du meridian electro acupuncture;  spinal cord injury;  apoptosis;  autophagy;  microtubule-associated protein 1 light chain 3-Ⅱ
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨电针治疗对脊髓损伤大鼠神经细胞自噬、凋亡及运动功能的影响。方法:将36只SD大鼠按照随机数字表法分为假手术组、脊髓损伤组、督脉电针组,每组12只。采用MASCIS Impacto1精确撞击制作脊髓损伤模型。假手术组只行椎板切除术,不造成脊髓损伤; 脊髓损伤组采用MASCIS Impacto1法制作脊髓损伤模型,不进行干预治疗; 督脉电针组在脊髓损伤后给予督脉电针治疗。于损伤后第1,3,7天分别对各组大鼠进行BBB运动功能评分。损伤后第7天取脊髓组织,用Western Blot法检测微管相关蛋白1轻链3(LC3)-Ⅱ的变化,用TUNEL法检测细胞凋亡情况。结果:损伤后第3天和第7天,督脉电针组BBB评分为(5.8±0.9)和(9.5±0.9),与脊髓损伤组(4.1±0.8)和(6.4±0.7)分比较显著升高,差异有统计学意义(P<0.05),而术后第1天两者评分为(2.8±0.7)和(2.4±0.5),差异无统计学意义(P>0.05)。与假手术组相比,脊髓损伤组LC3-Ⅱ表达明显升高,差异有统计学意义(P<0.05),细胞凋亡显著增加,差异有统计学意义(P<0.05); 与脊髓损伤组相比,督脉电针组LC3-Ⅱ显著升高,差异有统计学意义(P<0.05),细胞凋亡显著降低,差异有统计学意义(P<0.05)。结论:督脉电针治疗能增强脊髓损伤大鼠神经细胞自噬,减少凋亡,改善大鼠运动功能。
Abstract:
Objective:To explore the effect of electro acupuncture(EA)onautophagy,apoptosis and locomotor function after spinal cord injury in rats.Methods:Thirty-six adult female SD rats were randomly divided into three groups:Sham surgery group,spinal cord injury(SCI)group and Du meridian EA group,with 12 rats in each group.Spinal cord injury model was established using precision impacting with MASCIS Impactol.The Sham group only received laminectomy.The rats in SCI group were sustained SCI and were given no intervention.The rats in Du meridian EA group received Du meridian EA treatment after SCI.Scores of Basso Beattie Bresnahan(BBB)motor functions were assessed at 1,3,7 d after the injury.Then the rats were sacrificed at 7 d after the injury,the expression of LC3-Ⅱ in spinal cord was detected with Western Blot,and the number of apoptotic cells were assessed with TUNEL staining.Results:Scores of BBB scale at 3,7 d after injury in Du meridian EA group(5.8±0.9,9.5±0.9)were increased compared with those in Sham group(4.1±0.8,6.4±0.7)(P<0.05),but there were no significant difference between two groups(2.8±0.7 vs 2.4±0.5,P>0.05)at 1 d after injury.The expression of LC3-Ⅱ and the number of apoptotic cells in SCI group were increased compared with those in Sham group(P<0.05).The expression of LC3-Ⅱ were increased while the number of apoptotic cells were decreased in Du meridian EA group compared with those in SCI group(P<0.05).Conclusion:Du meridian electro acupuncture may enhance autophagy and inhibit apoptosisto improve the locomotor function after spinal cord injury in rats.

参考文献/References:

[1] ALLISON D J,GABRIEL D A,KLENTROU P,et al.The influence of chronic inflammation on peripheral motor nerve conduction following spinal cord injury:a randomized clinical trial[J].Topics in Spinal Cord Injury Rehabilitation,2017,23(4):377-385. [2] BIGFORD G,NASH M S.Nutritional health considerations for persons with spinal cord injury[J].Topics in Spinal Cord Injury Rehabilitation,2017,23(3):188-206. [3] DUMONT R J,VERMA S,OKONKWO D O,et al.Acute spinal cord injury,part Ⅱ:contemporary pharmacotherapy[J].Clinical Neuropharmacology,2001,24(5):265-279. [4] KANNO H,OZAWA H,SEKIGUCHI A,et al.Induction of autophagy and autophagic cell death in damaged neural tissue after acute spinal cord injury in mice[J].Spine(Phila Pa 1976),2011,36(22):1427-1434. [5] 于晨,刘刚,刘佳,等.脊髓损伤中细胞自噬的研究进展[J].临床和实验医学杂志,2014,13(19):1645-1648. [6] WANG Z Y,LIU W G,MUHARRAM A,et al.Neuroprotective effects of autophagy induced by rapamycin in rat acute spinal cord injury model[J].Neuroimmunomodulation,2014,21(5):257-267. [7] YU H,LI L,LIU R,et al.Autophagy in long propriospinal nell-rons is activated after spinal cord injury in adult rats[J].Neurosei Lett,2016,634(12):138-145. [8] JIN H P,KIM J H,OH S K,et al.Analysis of equivalent parameters of two spinal cord injury devices:the New York university impactor versus the infinite horizon impactor[J].Spine Journal,2016,16(11):1392-1403. [9] 张林,孙忠人,陈晨,等.近10年电针治疗脊髓损伤机制的实验研究进展[J].针灸临床杂志,2012,28(1):62-65. [10] 谭龙旺,张海波,杨利学,等.电针联合嗅鞘细胞移植对大鼠脊髓损伤区脑源性神经营养因子表达的影响[J].中国中医骨伤科杂志,2014,22(8):4-8. [11] 李华南,张海明,顾兵,等.针刺促进脊髓损伤后神经功能恢复的机制及相关信号通路的作用[J].中国康复理论与实践,2017,23(6):641-644. [12] GAO S,ZHANG Z M,SHEN Z L,et al.Atorvastatin activates autophagy and promotes neurological function recovery after spinal cord injury[J].Neural Regeneration Research,2016,11(6):977-982. [13] SCHAAF M B E,KEULERS T G,VOOIJS M A,et al.LC3/GABARAP family proteins:autophagy-(un)related functions[J].The FASEB Journal,2016,30(12):3961-3978. [14] MIZUSHIMA N,KOMATSU M.Autophagy:renovation of cells and tissues[J].Cell,2011,147(4):728-741. [15] KABEYA Y,MIZUSHIMA N,UENO T,et al.LC3,a mammalian homologue of yeastApg8p,is localized in autophagosome membranes after processing[J].EMBO J,2000,19(21):5720-5728. [16] 王延雷,齐英娜,董春科,等.脊髓减压联合督脉电针对急性脊髓压迫损伤大鼠疗效影响的实验研究[J].中国中医骨伤科杂志,2018,26(2):3-7. [17] 李晓宁,单筱淳,吴磊,等.急性脊髓损伤后最佳干预时间窗的实验研究[J].中国中医急症,2017,26(7):1213-1214. [18] ZHAO H,JAFFER T,EGUCHI S,et al.Role of necroptosis in the pathogenesis of solid organ injury[J].Cell Death and Disease,2015,6(11):e1975. [19] 齐英娜,谭明生,王延雷,等.补阳还五汤对急性脊髓损伤大鼠内质网应激相关因子影响的研究[J].中国中医骨伤科杂志,2018,26(2):8-12. [20] CHEN H C,FONG T H,HSU P W,et al.Multifaceted effects of rapamycin on functional recovery after spinal cord injury in rats through autophagy promotion,anti-inflammation,and neuroprotection[J].Journal of Surgical Research,2013,179(1):203-210.

备注/Memo

备注/Memo:
基金项目:浙江省医药卫生科技计划项目(2017KY482) △通信作者 E-mail:tuwenzhan@163.com(收稿日期:2019-07-06)
更新日期/Last Update: 2019-10-30