[1]严可 欧梁 邝高艳 谢佳佳 卢敏 王林华△.加味独活寄生汤对兔膝骨关节炎软骨细胞凋亡的影响[J].中国中医骨伤科杂志,2019,27(04):1-6.
　YAN Ke OU Liang KUANG Gaoyan XIE Jiajia LU Min WANG Linhua.Influence of Modified Duhuo Parasitic Mixture on Chondrocyte Apoptosis in Rabbit Knee Osteoarthritis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2019,27(04):1-6.
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加味独活寄生汤对兔膝骨关节炎软骨细胞凋亡的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第27卷
期数:
2019年04期
页码:
1-6
栏目:
实验研究
出版日期:
2019-04-12

文章信息/Info

Title:
Influence of Modified Duhuo Parasitic Mixture on Chondrocyte Apoptosis in Rabbit Knee Osteoarthritis
文章编号:
1005-0205(2019)04-0001-06
作者:
严可1 欧梁1 邝高艳1 谢佳佳1 卢敏1 王林华1△
1湖南中医药大学第一附属医院(长沙,410007)
Author(s):
YAN Ke1 OU Liang1 KUANG Gaoyan1 XIE Jiajia1 LU Min1 WANG Linhua1△
1First Affiliated Hospital, Hunan University of Traditional Chinese Medicine,Changsha 410007,China.
关键词:
加味独活寄生汤;  膝骨关节炎;  细胞凋亡;  实验研究
Keywords:
modified Duhuo parasitic mixture;  knee osteoarthritis;  apoptosis;  experimental study
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨加味独活寄生汤对兔膝骨关节炎软骨细胞凋亡的作用机制。方法:将新西兰大白兔32只随机分组为空白组、模型组、加味独活寄生汤组、氨基葡萄糖组,每组8只; 造模后予加味独活寄生汤组3.4 mL/kg的剂量灌胃给药,空白组、模型组、氨基葡萄糖组等剂量生理盐水溶解灌胃。观察体征、体质量、活动情况,采用TUNEL法检测软骨细胞凋亡情况,RT-PCR 检测Wnt/β-catenin等mRNA表达情况。结果:加味独活寄生汤组体征表现有明显改善,TUNEL法阳性细胞计数结果显示:与空白组比较,模型组、加味独活寄生汤组和氨基葡萄糖组软骨中TUNEL法阳性细胞数量明显增加,差异有统计学意义(P<0.05); 与模型组比较,加味独活寄生汤组和氨基葡萄糖组软骨中TUNEL法凋亡的阳性细胞数量明显减少,差异有统计学意义(P<0.05)。Wnt3a,β-catenin及MMP-13的表达:与空白组比较,模型组、加味独活寄生汤组和氨基葡萄糖组的Wnt3a,β-catenin及MMP-13 mRNA表达显著升高,差异有统计学意义(P<0.05); 与模型组比较,加味独活寄生汤组和氨基葡萄糖组的Wnt3a,β-catenin及MMP-13 mRNA表达均明显下调,差异有统计学意义(P<0.05)。结论:加味独活寄生汤通过调控Wnt/β-catenin通路和下游因子MMP-13等相关基因表达,从而抑制膝关节软骨细胞凋亡,可能是加味独活寄生汤治疗骨关节炎的作用机制。
Abstract:
Objective:To investigate the mechanism of modified Duhuo parasitic mixture on chondrocyte apoptosis in rabbit knee osteoarthritis.Methods:Thirty-two New Zealand white rabbits were randomly divided into blank group, model group, modified Duhuo parasitic mixture group, glucosamine group(8 in each group).The dosage of 3.4 mL/kg in the group of modified Duhuo parasitic mixture was given by gavage.In the glucosamine group, 5 mg/kg was dissolved and perfused with normal saline at the same dose to observe the signs, weights and activities.TUNEL method was used to detect the apoptosis of chondrocytes, in RT-PCR,the expression of Wnt/β-catenin and mRNA were detected.Results:The physical signs of rabbits in the modified Duhuo parasitic mixture group were obviously improved.The results of TUNEL positive cell count showed that,compared with the blank group, the model group,the number of TUNEL positive cells in cartilage of modified Duhuo parasitic mixture group and glucosamine group were significantly increased(P<0.05).Compared with the model group, the number of TUNEL apoptotic positive cells in the modified Duhuo parasitic mixture group and the glucosamine group were significantly decreased(P<0.05), and Wnt3a, β-catenin,MMP-13 were also found in the cartilage group(P<0.05).Compared with blank group, the expression of Wnt3a, β-catenin,MMP-13 mRNA in model group, modified Duhuo parasitic mixture group and glucose group were significantly increased(P<0.05).Compared with the model group, the expression of Wnt3a, β-catenin,MMP-13 mRNA in the modified Duhuo parasitic mixture group and glucose group were significantly decreased(P<0.05).Conclusion:Modified Duhuo parasitic mixture can inhibit the fine cartilage of knee joint by regulating the expression of Wnt/β-catenin pathway and downstream factor MMP-13.Apoptosis may be the mechanism of modified Duhuo parasitic mixture in the treatment of osteoarthritis.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(81574004,81874476) 湖南省中医药科研计划项目(2017149) 湖南省教育厅项目(17C1224) △通信作者 E-mail:155053648@qq.com
更新日期/Last Update: 2019-04-12