[1]汪小飞 李晶晶.淫羊藿总黄酮对老年骨质疏松大鼠Notch和Smads通路蛋白表达的影响[J].中国中医骨伤科杂志,2019,27(02):1-5.
　WANG Xiaofei LI Jinjin.The Influence of Total Flavonoids from Herba Epimedii on theProtein Expression of Notch and Smads SignalPathway in Senile Osteoporosis Rats Model[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2019,27(02):1-5.
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淫羊藿总黄酮对老年骨质疏松大鼠Notch和Smads通路蛋白表达的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第27卷
期数:
2019年02期
页码:
1-5
栏目:
实验研究
出版日期:
2019-02-15

文章信息/Info

Title:
The Influence of Total Flavonoids from Herba Epimedii on theProtein Expression of Notch and Smads SignalPathway in Senile Osteoporosis Rats Model
文章编号:
1005-0205(2019)02-0001-05
作者:
汪小飞1 李晶晶2
1浙江中医药大学附属第一医院药剂科(杭州,310006)2浙江省立同德医院药学部
Author(s):
WANG Xiaofei1 LI Jinjin2
1The First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310006,China; 2Tongde Hospital of Zhejiang Province,Hangzhou 310012,China.
关键词:
骨质疏松;  淫羊藿总黄酮;  骨密度;  蛋白;  通路
Keywords:
osteoporosis;  total flavonoids from herba epimedii;  bone mineral density;  Notch1 protein;  Smads protein
分类号:
R-33
文献标志码:
A
摘要:
观察淫羊藿总黄酮对老年骨质疏松大鼠Notch和Smads通路蛋白表达的影响。方法:将60只老年雄性SD大鼠随机分为空白对照组、模型对照组、试验组(包括低、中、高剂量组)和阳性对照组共6组,试验组根据淫羊藿总黄酮灌胃剂量分为低剂量组、中剂量组和高剂量组,除空白对照组外,其他5组腹腔注射D-半乳糖建立老年骨质疏松大鼠模型,造模8周后连续灌胃给药12周,观察各组大鼠的骨密度和股骨抗弯力,ELISA法检测血清碱性磷酸酶(ALP)、骨保护素(OPG)及25-羟基维生素D(25OHD)含量,免疫组化法检测骨组织Notch1蛋白表达,免疫荧光法检测Smad4和Smad7,并比较肝肾功能指标变化。结果:试验组和阳性对照组的骨密度、股骨抗弯力、OPG和25OHD显著高于模型对照组,而ALP显著低于模型对照组,差异均有统计学意义(P<0.001),但试验高剂量组和阳性对照组比较,差异无统计学意义(P>0.05)。试验组和阳性对照组的Notch1和Smad4蛋白表达显著高于模型对照组,而Smad7蛋白表达低于模型对照组,差异均有统计学意义(P<0.001),但试验高剂量组和阳性对照组比较,差异无统计学意义(P>0.05)。结论:淫羊藿总黄酮可增加老年骨质疏松大鼠骨密度,改善骨代谢,可能与上调Notch1和Smad4蛋白表达及下调Smad7蛋白表达有关。
Abstract:
To investigate the influence of total flavonoids from herba epimedii(TFHE)on the protein expression of Notch and Smads signal pathway in senile osteoporosis rats model. Methods:Sixty senile male SD rats were randomly divided into the blank control group, model control group, treatment group and positive control group, and the treatment group were divided into low group, middle group and high group. Excepted the blank control group, the other groups were given intraperitoneal injection of D-galactose to establish the senile osteoporosis rats model. After 8 weeks, all groups were given drugs by lavage continuous 12 weeks, the bone mineral density(BMD)and bending force of femur were observed. The levels of serum alkaline phosphatase(ALP), bone protection element(OPG), 25-hydroxy vitamin D(25OHD)were detected by ELISA method, Notch1 were detected by immunohistochemical method, and the expression of Smad4 and Smad7 were tested by immunofluorescence, the liver and kidney function index changes were compared. Results:The BMD, bending force of femur, OPG and 25OHD in the experimental group and positive control group were significantly higher than the model control group, the ALP was significantly lower than the model control group, the differences were statistically significant(P<0.001), but the experimental-H group and positive control group was no statistically significant difference(P>0.05). The protein expression of Notch1 and Smad4 in experimental group and positive control group were significantly higher than the model control group, and the Smad7 was lower than the model control group, the difference were statistical significance(P<0.001), but the Experimental-H group and positive control group was no statistically significant difference(P>0.05). Conclusion:TFHE can increase the senile osteoporosis rats BMD, improve bone metabolism, may be related to increase Notch1 and Smad4 protein expression, decrease Smad7 protein expression.

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备注/Memo

备注/Memo:
1浙江中医药大学附属第一医院药剂科(杭州,310006)2浙江省立同德医院药学部
更新日期/Last Update: 2019-02-15