[1]刘钟 杨依然 王均华 张佳锋 史晓林△.益气温经方对CKIP-1介导的破骨细胞凋亡的影响[J].中国中医骨伤科杂志,2018,26(08):14-17.
　LIU Zhong YANG Yiran WANG Junhua ZHANG Jiafeng SHI Xiaolin.Effect of Yiqiwenjing Prescription on Osteoclasts Apoptosis Mediated by CKIP-1[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2018,26(08):14-17.
点击复制

益气温经方对CKIP-1介导的破骨细胞凋亡的影响()
分享到：

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第26卷
期数:
2018年08期
页码:
14-17
栏目:
实验研究
出版日期:
2018-08-02

文章信息/Info

Title:
Effect of Yiqiwenjing Prescription on Osteoclasts Apoptosis Mediated by CKIP-1
文章编号:
1005-0205(2018)08-0014-04
作者:
刘钟1 杨依然1 王均华1 张佳锋1 史晓林2△
1浙江中医药大学(杭州,310053) 2浙江中医药大学附属第二医院
Author(s):
LIU Zhong1 YANG Yiran1 WANG Junhua1 ZHANG Jiafeng1 SHI Xiaolin2△
1Zhejiang Chinese Medical University, Hangzhou 310053, China; 2The secand ffiliared tespital of Zhejiang Chinese Medical University,Hangzhou 310005, China.
关键词:
益气温经方;  CKIP-1;  破骨细胞;  凋亡
Keywords:
Keywords:Yiqiwenjing prescription;  casein kinase 2 interaction protein 1;  osteoclasts;  apoptosis
分类号:
R-33
文献标志码:
A
摘要:
目的:观察益气温经方对酪蛋白激酶2相互作用蛋白1(Casein Kinase 2 Interaction Protein 1, CKIP-1)介导的破骨细胞凋亡程度的影响。方法:从SD乳鼠中分离破骨细胞体外培养,建立CKIP-1过表达和低表达载体,病毒包装构建稳定表达的细胞系。分为以下5组:破骨细胞对照组(A组),破骨细胞+空载病毒组(B组),破骨细胞+CKIP-1过表达病毒组(C组),破骨细胞+CKIP-1过表达病毒+益气温经方处理组(D组),破骨细胞+CKIP-1低表达病毒组(E组)。病毒感染96 h后,Annexin V-FITC/PI检测细胞凋亡。结果:在破骨细胞中,与A组相比,过表达CKIP-1病毒组凋亡细胞比例显著降低,差异有统计学意义(P=0.017),而益气温经方处理后,凋亡细胞比例有升高的趋势,与A组相比差异无统计学意义(P=0.059),与C组相比差异有统计学意义(P=0.000 6); 与A组相比,低表达CKIP-1组凋亡细胞比例显著升高,差异有统计学意义(P<0.000 1)。结论:CKIP-1具有抑制破骨细胞凋亡的功能,益气温经方可能通过逆转这种趋势,抑制骨吸收,从而起到治疗骨质疏松的作用。
Abstract:
Abstract Objective:To observe the effect of Yiqiwenjing prescription on osteoclasts apoptosis mediated by casein kinase 2 interaction protein 1(CKIP-1).Methods: Osteoclasts were isolated from SD neonatal rats in vitro, we established CKIP-1 overexpression and low expression vector.With the method of virus packaging, stable expressed cell lines were established.Then these cell lines were divided into the following 5 groups:group A, osteoclasts control group; group B, osteoclasts & vector group; group C, osteoclasts & CKIP-1 overexpression virus group; group D, osteoclasts & CKIP-1 overexpression virus & Yiqiwenjing prescription intervened group; group E, osteoclasts & CKIP-1 low expression virus group.96h after virus infection, Annexin V-FITC/PI was used to detect apoptosis.Results: Among osteoclasts, compared with the group A, the proportion of apoptotic cells in CKIP-1 overexpression virus group was significantly reduced(P=0.017).After intervened by Yiqiwenjing prescription, the proportion of apoptotic cells increased, and there were no significant difference compared with group A(P=0.059), while significant difference compared with group C(P=0.000 6).The proportion of apoptotic cells showed significantly increased compared with group A(P<0.000 1).Conclusion: CKIP-1 has the function of inhibiting the apoptosis of osteoclasts, and Yiqiwenjing prescription's effect may lie in reversing this trend, inhibiting bone absorption, and treating osteoporosis.

参考文献/References:

[1] 姚建亮, 王博, 吴鹏,等.强骨饮对去卵巢大鼠骨折愈合骨小梁Micro-CT改变研究[J].中国骨质疏松杂志, 2016, 22(11):1395-1398. [2] 张志强, 李春雯, 潘定权,等.蛋白组学在骨质疏松中的应用[J].中华中医药学刊, 2014,32(6):1316-1319. [3] 胡炯, 王博, 吴鹏,等.CKIP-1与骨质疏松的最新研究进展[J].中国骨质疏松杂志, 2016, 22(8):1053-1057. [4] Iida T, Wagatsuma K, Hirayama D, et al.Is osteopontin a friend or foe of cell apoptosis in inflammatory gastrointestinal and liver diseases?[J].International Journal of Molecular Sciences, 2018, 19(1). [5] Ye T, Cao P, Qi J, et al.Protective effect of low-dose risedronate against osteocyte apoptosis and bone loss in ovariectomized rats[J].Plos One, 2017, 12(10):e0186012. [6] Chen SY, Shiau AL, Wu CL, et al.P53-derived hybrid peptides induce apoptosis of synovial fibroblasts in the rheumatoid joint[J].Oncotarget, 2017, 8(70):115413-115419. [7] Wang L, Zhang HY, Gao B, et al.Tetramethylpyrazine protects against glucocorticoid-induced apoptosis by promoting autophagy in mesenchymal stem cells and improves bone mass in glucocorticoid-induced osteoporosis rats[J].Stem Cells & Development, 2017, 26(6):419-430. [8] Chen GM, Ding RF, Tan YD, et al.Role of the CKIP1 gene in proliferation and apoptosis of the human lung cancer cell line H1299[J].Genetics & Molecular Research Gmr, 2015, 14(2):4005-4014. [9] 王秉义, 潘剑.丹参素拮抗氧化应激所致骨质疏松并通过PI3k/Akt通路减少成骨细胞的凋亡[J].中国骨质疏松杂志, 2017, 23(1):1-5. [10] 王晓晖, 罗向霞, 史晓伟,等.葛根素对高糖培养成骨细胞增殖、矿化及凋亡的影响[J].中国中医药科技, 2017,24(6):733-735. [11] 明磊国, 王鸣刚, 陈克明,等.蛇床子素对体外培养破骨细胞骨吸收及细胞凋亡的影响[J].药学学报, 2012,47(2):174-179. [12] 邱慈鑫.温阳补肾方对体外培养破骨细胞活性与凋亡影响的实验研究[D].福州:福建中医药大学, 2015. [13] Chen GM, Ding RF, Tan YD, et al.Role of the CKIP1 gene in proliferation and apoptosis of the human lung cancer cell line H1299[J].Genetics & Molecular Research Gmr, 2015, 14(2):4005-4014. [14] Peng X, Wu X, Zhang J, et al.The role of CKIP-1 in osteoporosis development and treatment[J].Bone & Joint Research, 2018, 7(2):173-178. [15] Laplante M, Sabatini DM.mTOR signaling in growth control and disease[J].Cell, 2012, 149(2):274-293.

备注/Memo

备注/Memo:
△通信作者 E-mail:xlshi-2002@163.com
更新日期/Last Update: 2018-08-02