[1]张翔 董晓俊 吴泱△ 齐理华.早期激素性股骨头缺血性坏死动物模型的建立研究[J].中国中医骨伤科杂志,2018,26(02):67-71.
　ZHANG Xiang DONG Xiaojun WU Yang QI Lihua.Model Establishment of Early Steroid-Induced Avascular Necrosis of Femoral Head in Rabbit[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2018,26(02):67-71.
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早期激素性股骨头缺血性坏死动物模型的建立研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第26卷
期数:
2018年02期
页码:
67-71
栏目:
实验研究
出版日期:
2018-01-15

文章信息/Info

Title:
Model Establishment of Early Steroid-Induced Avascular Necrosis of Femoral Head in Rabbit
文章编号:
1005-0205(2018)02-0067-05
作者:
张翔1 董晓俊2 吴泱1△ 齐理华3
1浙江中医药大学附属温州市中西医结合医院 (浙江 温州,325000) 2武汉市中医医院 3湖北中医药大学
Author(s):
ZHANG Xiang1 DONG Xiaojun2 WU Yang1△ QI Lihua3
1Integrated Traditional Chinese and Western Medicine Hospital of Wenzhou,Affiliated Hospital of Zhejiang Chinese Medicine University,Wenzhou 325000,Zhejiang China; 2Wuhan Hospital of Traditional Chinese Medicine,Wuhan 430014,China;
关键词:
早期激素性股骨头缺血坏死;  动物模型;  病理学检查;  死亡原因
Keywords:
Keywords: early steroid-Induced avascular necrosis;  animal model;  pathological examination;  death factors
分类号:
R-33
文献标志码:
A
摘要:
目的:采取内毒素加激素诱导建立早期激素性股骨头缺血坏死动物模型,采取病理学检查和影像学检查评价其可靠性,以及探讨造模过程中动物死亡原因。方法:20只健康成年白兔,体质量(2.5±0.2)kg,随机分为对照组、实验组,每组10只。实验组兔耳缘静脉注射LPS(10 μg/kg),24 h后再次给药1次; 在第2次注射LPS后,立刻臀肌注射醋酸泼尼松龙注射液(25 mg/kg),共3次。对照组臀肌注射等量生理盐水(10 mg/kg)。造模过程中记录动物一般情况,造模6周后行股骨头病理组织学检查及影像学检查。结果:通过病理组织学检查实验组骨小梁缩小,稠密度降低,局部断裂,甚至可见结缔组织包绕形成云片状; 骨细胞核固缩,空骨陷窝明显增多; 骨髓腔内脂肪细胞增多、增大; 骨髓受压变小。实验组死亡动物肝组织广泛水肿,组织充血,门管区炎性细胞浸润,部分细胞气球样变坏死。对照组和实验组空骨陷窝率分别为(9.368±2.310)%和(32.500±3.479)%,差异有统计学意义(P<0.01)。结论:内毒素加激素诱导可短期内建立动物模型,操作简便,重复性高,同时形成动物体内高凝血状态,更加符合激素性股骨头缺血性坏死临床病理特征。
Abstract:
Abstract Objective:To establish a rabbit model of steroid-induced avascular necrosis of the femoral head(SANFH)by LPS and sodium phosphate solution and to evaluate its validity and death factors with pathological examination.Methods:Twenty healthy adult rabbits with body weight of(2.5±0.2)kg were randomly divided into control group and model group,10 rabbits in each group.The model group received one intravenous injection of LPS(10 μg/kg),and it was repeated after 24 h.After the second injection of LPS,1 injection of dexamethasone sodium phosphate solution(25 mg/kg)was given intramuscularly immediately.The control group was injected with equal amount of saline,every day for 3 times.The general conditions of rabbits were recorded during modeling.The histopathological examination and imaging examination of the femoral head were made after 6weeks.Results: The histopathological examination indicated that the bone trabecula in model group shrinked,collapsed locally and decreased in bone density,and even it was surrounded by connective tissue form a cloud sheet.Light microscopy showed that the nueleus of osteocytes was compressed and the empty lacuna and the fatty cells increased,with the decreasing of bone marrow.Pathological changes in the experimental group included widely liver tissue edema,hyperemia,inflammatory cells infiltration within the portal area,ballooning changes and necrosis in liver cells.The rates of empty lacunae were(9.368±2.310)% in control group and(32.500±3.479)%in model group,showing significant difference(P<0.01).Conclusion:Endotoxin with hormone induction,which is simple and reproducible,can set up animal models in a short time.And the high blood coagulation state of the animal is more in line with the pathological characteristics of steroid-induced avascular necrosis of femoral head.

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备注/Memo

备注/Memo:
基金项目:湖北省中医药中西医结合科研项目(2013Z-Y12) △通信作者 E-mail:173552256@qq.com
更新日期/Last Update: 2018-02-15