[1]余光书 熊国胜 熊圣仁 林焱斌△.基于质谱技术分析软骨细胞线粒体蛋白质及细胞凋亡机制[J].中国中医骨伤科杂志,2018,26(01):1-4.
　YU Guangshu XIONG Guosheng XIONG Shengren LIN Yanbin.Mitochondrial Protein Research in Chondrocytes and Analysis of Cell Apoptosis Mechanism Based on Mass Spectrometry[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2018,26(01):1-4.
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基于质谱技术分析软骨细胞线粒体蛋白质及细胞凋亡机制()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第26卷
期数:
2018年01期
页码:
1-4
栏目:
实验研究
出版日期:
2018-01-15

文章信息/Info

Title:
Mitochondrial Protein Research in Chondrocytes and Analysis of Cell Apoptosis Mechanism Based on Mass Spectrometry
文章编号:
1005-0205(2018)01-0001-04
作者:
余光书1 熊国胜1 熊圣仁1 林焱斌1△
1厦门大学附属福州第二医院骨科(福州,350007) △通信作者 E-mail:812953900@qq.com
Author(s):
YU Guangshu1 XIONG Guosheng1 XIONG Shengren1 LIN Yanbin1△
1Department of Orthopedics,Fuzhou Second Hospital Affiliated to Xiamen University,Fuzhou Fujian 350007,China.
关键词:
串联质谱标签(TMT);  软骨细胞;  线粒体;  蛋白质组学
Keywords:
Keywords: tandem mass tags(TMT);  chondrocytes;  mitochondria;  proteomics
分类号:
R-33
文献标志码:
A
摘要:
目的:通过定性观察膝骨性关节炎软骨细胞线粒体中的蛋白质,进一步探讨软骨细胞凋亡的分子机制。方法:将收集到的膝关节软骨组织进行线粒体提取,并采用定量蛋白质组学串联质谱标签标记技术进行检测,再通过搜索Uniprot数据库和利用Proteome Discoverer软件进行分析,从而进一步分析其生物学信息。结果:通过数据库检索得到294个蛋白质点,其中有14种蛋白质点定位于线粒体,另外根据数据库及线粒体的结构可能有11种定位于线粒体。通过数据库的筛选、分析、分类,归纳出的蛋白质有ATP合酶、超氧化物歧化酶、谷氨酸脱氢酶1、谷胱甘肽S-转移酶、天冬氨酸转氨酶、胸苷激酶2、苹果酸脱氢酶、补体、细胞色素b-c1、热休克蛋白10 kDa、热休克蛋白60 kDa、Trifunctional enzyme及Stress-70 protein.另外,通过蛋白复合物的生物学进程、细胞成分及分子功能的分析,可能存在的信号通路有BMP4-BGN与Cofilin-actin-CAP1.结论:对软骨细胞线粒体中的蛋白质作全面的定性观察有助于进一步了解软骨细胞凋亡的分子机制,从而为骨性关节炎寻找更好的预测指标和干预靶点。
Abstract:
Abstract Objective:To explore the molecular mechanism of chondrocytes apoptosis by observing the proteins of chondrocytes of knee osteoarthritis.Methods:Mitochondria were extracted from the organization of knee osteoarthritis,and were detected by the quantitative proteomics TMT technology.Then the Uniprot database and Proteome Discoverer software were applied to analyze the bioinformatics.Results:All 294 protein spots were retrieved from the database.14 protein spots were located in mitochondria,and 11 protein spots were expected to be located in mitochondria according to the structure of the mitochondria and database.ATP synthase,superoxide dismutase,glutamate dehydrogenase 1,glutathione s-transferase p,aspartate aminotransferase,thymidine kinase 2,malate dehydrogenase,complement,cytochrome b-c1,10 kDa heat shock protein,60 kDa heat shock protein,trifunctional enzyme subunit alpha,stress-70 protein was summarized by screening,analyzing and classifying based on database.In addition,the signaling pathways of BMP4-BGN and Cofilin-actin-CAP1 may be in mitochondria by analyzing of biological process,cellular component,and molecular function.Conclusion:The molecular mechanisms of chondrocyte apoptosis is further understood by studying the proteins of chondrocytes of knee osteoarthritis,which will be used to screen the diagnostic biomarkers and related to therapeutic targets.

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备注/Memo

备注/Memo:
基金项目:福建省自然科学基金资助项目(2016J01597) 福州市科技计划项目(2017-S-130-5) 福州市卫生计生科技计划项目(2016-S-wq2)
更新日期/Last Update: 2018-01-15