[1]吕江华 杨华瑞 刘万军 刘健 陈园 陈江水 方志 邹亮 鄢飞 舒讯 杨宜 龚春龙 尧浩 赵龙桃 熊炎强 鲍同柱△.栀子蜂蜜膏外敷治疗兔急性软组织损伤的实验研究[J].中国中医骨伤科杂志,2017,25(08):6-10.
　LV Jianghua YANG Huarui LIU Wanjun LIU Jian CHEN Yuan CHEN Jiangshui FANG Zhi ZOU Liang YAN Fei SHU Xun YANG Yi GONG Chunlong YAO Hao ZHAO Longtao XIONG Yanqiang BAO Tongzhu.Effect of Gardenia Honey Cream External Application on Acute Soft Tissue Injury in Rabbits[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2017,25(08):6-10.
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栀子蜂蜜膏外敷治疗兔急性软组织损伤的实验研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第25卷
期数:
2017年08期
页码:
6-10
栏目:
实验研究
出版日期:
2017-08-03

文章信息/Info

Title:
Effect of Gardenia Honey Cream External Application on Acute Soft Tissue Injury in Rabbits
文章编号:
1005-0205(2017)08-0006-05
作者:
吕江华1 杨华瑞2; 3 刘万军2; 3 刘健2; 3 陈园4 陈江水2; 3 方志2; 3 邹亮2; 3 鄢飞2; 3 舒讯2; 3 杨宜2; 3 龚春龙2; 3 尧浩2; 3 赵龙桃2; 3 熊炎强5 鲍同柱2; 3△
1.湖北枝江市人民医院(湖北 枝江,443200)
2.三峡大学第一临床医学院骨科
3.三峡大学创伤研究所
4.三峡大学第一临床医学院急诊科
5.三峡大学肿瘤微环境与免疫治疗湖北省重点实验室
△.通信作者 E-mail:baotongzhu110@163.com
Author(s):
LV Jianghua1 YANG Huarui2; 3 LIU Wanjun2; 3 LIU Jian2; 3 CHEN Yuan4 CHEN Jiangshui2; 3 FANG Zhi2; 3 ZOU Liang2; 3 YAN Fei2; 3 SHU Xun2; 3 YANG Yi2; 3 GONG Chunlong2; 3 YAO Hao2; 3 ZHAO Longtao2; 3 XIONG Yanqiang5 BAO Tongzhu2; 3△
关键词:
栀子;  蜂蜜膏;  软组织损伤;  炎性因子
Keywords:
gardenia;  soft tissue;  damage points;  inflammatory factor
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨栀子蜂蜜膏对兔急性软组织损伤的影响。方法:34只新西兰大白兔,随机分为正常组4只,实验组10只,扶他林组10只,对照组10只。采取改良击打法制造兔急性软组织损伤模型,造模30 min后,实验组外敷栀子蜂蜜膏,扶他林组外用涂抹扶他林,对照组外敷生理盐水纱布,外敷面积略大于击打面积,2次/d.分别于造模后、给药第3天、给药第7天做局部损伤病理组织切片,并记录每天的损伤积分。ELISA法测定各组损伤组织中给药第3天、第7天IL-1及TNF-α含量。各项数据釆用(x^-&#177;s)表示,用SPSS 18.0软件进行t检验,P<0.05差异有统计学意义。结果:实验组在局部外用栀子蜂蜜膏后,软组织损伤积分低于对照组,差异有统计学意义(P<0.05),并且优于扶他林组; 实验组局部外用栀子蜂蜜膏第3天和第7天后,与对照组比较,局部损伤软组织病理形态学有明显改善。在给药第3天和第7天后,实验组兔局部损伤组织内IL-1含量高于正常组,差异有统计学意义(P<0.01,P<0.01),低于对照组(P<0.01,P<0.01),并且低于扶他林组,差异有统计学意义(P<0.05,P<0.05); 在给药第3天和第7天后,实验组兔局部损伤软组织内TNF-α含量高于正常组,差异有统计学意义(P<0.01,P<0.01),低于对照组,差异有统计学意义(P<0.01,P<0.01),并且低于扶他林组,差异有统计学意义(P<0.05,P<0.05)。结论:栀子蜂蜜膏可以明显改善兔急性软组织损伤局部症状,可以减少兔局部损伤软组织(肌肉)炎性细胞浸润; 栀子蜂蜜膏可以降低兔局部损伤软组织中炎性因子IL-1及TNF-α的含量; 栀子蜂蜜膏治疗兔急性软组织损伤的机制可能是通过抑制局部损伤组织中IL-1及TNF-α的表达而起作用。
Abstract:
Objective:To investigate the effect of gardenia honey cream on acute soft tissue injury in rabbits.Methods:Thirty-four New Zealand white rabbits were randomly divided into normal group(n=4),control group(n=10),experimental group(n=10)and voltaren treated group(n=10).The model of acute soft tissue injury of rabbits was made by modified hitting method.After 30 minutes of modeling,the rabbits were applied with different drugs twice a day.The experimental group were treated with gardenia honey cream,voltaren treated group were treated with voltaren,control group was treated with saline,and the applied area was slightly larger than the hit area.The local injury pathological sections were taken respectively after the modeling,day 3 and day 7 after administration.Damage points were record daily.The levels of IL-1 and TNF-α on day 3 and day 7 after administration were determined by ELISA.The data were expressed as mean±standard deviation,with t test with SPSS 18.0 software,P<0.05 means statistically significant.Results:Damage points in the experimental group was lower than that in the control group(P<0.05),and was better than that in the voltaren treated group.Compared with the control group,local injury soft tissue pathology in the experimental group three days and seven days after administration were improved significantly.Compared with normal group,the level of IL-1 in experimental group was higher in three days and seven days after administration(P<0.01,P<0.01); but it was lower than that in voltaren treated group(P<0.05,P<0.05).The level of TNF-α in experimental group was higher in three days and seven days after administration than normal group(P<0.01,P<0.01),and it was lower than that in control group(P<0.01,P<0.01)and voltaren treated group(P<0.05,P<0.05).Conclusion:Gardenia honey cream can significantly improve the local symptoms of acute soft tissue injury in rabbits; and it can reduce the infiltration of inflammatory cells in soft tissue(muscle)of rabbits with local damage.It can also reduce the level of inflammatory factors IL-1 and TNF-α in soft tissue of rabbits with local injury.The mechanism of gardenia honey cream in the treatment of acute soft tissue injury in rabbits may be mediated by inhibition the expression of IL-1 and TNF-α in locally injured tissue.

参考文献/References:

[1] 庄洪,岑泽波,袁浩,等.外用双柏散浸剂治疗急性软组织损伤的实验研究[J].中医正骨,1992(2):1-4.
[2] 孙燕,廖怀章,刘绪银,等.活血化瘀利水方对急性软组织损伤模型大鼠IL-1β和PGE-2影响的实验研究[J].中国中医骨伤科杂志,2015,23(12):6-8.
[3] 韩学哲,崔刚,刘传镐,等.展筋活血散对大鼠软组织损伤的影响[J].中药药理与临床,2014,30(6):151-154.
[4] Bleakley CM,Glasgow P,Macauley DC.PRICE needs updating,should we call the POLICE?[J].Br J Sports Med,2012,46(4):220-221.
[5] Wolfe MW,Uhl TL,Mattacola CG,et al.Management of ankle sprains[J].Am Fam Physician,2001,63(1):93-104.
[6] 叶朝辉.《黄帝内经》论治筋伤探讨[J].河南中医,2013(10):1620-1623.
[7] 董静,叶锐彬,张志峰,等.郑氏伤科活络膏对急性伤筋大鼠的实验研究[J].辽宁中医药大学学报,2008,10(11):173-175.
[8] 薛彬,奚小冰,李飞跃.外敷药治疗软组织损伤的临床应用进展[J].中国中医骨伤科杂志,2014,22(6):78-80.
[9] 张彩丽,陈渭良.中药外治急性软组织损伤研究近况浅析[J].中国中医骨伤科杂志,2007,15(3):60-61.
[10] 张向荣,潘文军.生山栀外敷减轻创伤肿痛效果观察[J].中医正骨,2008,20(5):18-20.
[11] 南京中医药大学.中药大辞典[M].上海:上海科学技术出版社,2006.
[12] Damlar I,Esen E,Tatli U.Effects of glucosamine-chondroitin combination on synovial fluid IL-1beta,IL-6,TNF-alpha and PGE2 levels in internal derangements of temporomandibular joint[J].Med Oral Patol Oral Cir Bucal,2015,20(3):e278-e283.
[13] Ochman S,Frey S,Raschke MJ,et al.Local application of VEGF compensates callus deficiency after acute soft tissue trauma--results using a limb-shortening distraction procedure in rabbit tibia[J].J Orthop Res,2011,29(7):1093-1098.
[14] Wang L,Gao W,Xiong K,et al.VEGF and BFGF Expression and Histological Characteristics of the Bone-Tendon Junction during Acute Injury Healing[J].J Sports Sci Med,2014,13(1):15-21.
[15] Valenzuela A,Blanco J,Krust B,et al.Neutralizing antibodies against the V3 loop of human immunodeficiency virus type 1 gp120 block the CD4-dependent and independent binding of virus to cells[J].J Virol,1997,71(11):8289-8298.
[16] Ferreira SH,Lorenzetti BB,Bristow AF,et al.Interleukin-1 beta as a potent hyperalgesic agent antagonized by a tripeptide analogue[J].Nature,1988,334(6184):698-700.
[17] Hata H,Sakaguchi N,Yoshitomi H,et al.Distinct contribution of IL-6,TNF-alpha,IL-1,and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice[J].J Clin Invest,2004,114(4):582-588.
[18] Li X,Huang Q,Ong CN,et al.Chrysin sensitizes tumor necrosis factor-alpha-induced apoptosis in human tumor cells via suppression of nuclear factor-kappaB[J].Cancer Lett,2010,293(1):109-116.
[19] Singh U,Kumar A,Sinha R,et al.Calreticulin transacetylase catalyzed modification of the TNF-alpha mediated pathway in the human peripheral blood mononuclear cells by polyphenolic acetates[J].Chem Biol Interact,2010,185(3):263-270.
[20] Wilms H,Sievers J,Rickert U,et al.Dimethylfumarate inhibits microglial and astrocytic inflammation by suppressing the synthesis of nitric oxide,IL-1beta,TNF-alpha and IL-6 in an in-vitro model of brain inflammation[J].J Neuroinflammation,2010,7:30.
[21] Singh U,Kumar A,Sinha R,et al.Calreticulin transacetylase catalyzed modification of the TNF-alpha mediated pathway in the human peripheral blood mononuclear cells by polyphenolic acetates[J].Chem Biol Interact,2010,185(3):263-270.

备注/Memo

备注/Memo:
基金项目:湖北省自然科学基金(2013CFB220)
湖北省卫生计生西医类重点项目基金 (WJ2015MA025)
宜昌市卫生科研课题(A16-301-09)
更新日期/Last Update: 2017-08-15