[1]张韬 翁艳 陈冬冬 周燕芸 肖莉莉 张怡元△.Noggin基因过表达重组腺病毒载体的构建及其对骨肿瘤细胞MG63迁移侵袭能力的影响[J].中国中医骨伤科杂志,2017,25(07):11-15.
　ZHANG Tao WENG Yan CHEN Dongdong ZHOU Yanyun XIAO Lili ZHANG Yiyuan.Construction of Recombinant Adenovirus Vectors Overexpressing Noggin Gene and Its Effect on the Migration and Invasion Ability of Bone Tumor Cells MG63[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2017,25(07):11-15.
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Noggin基因过表达重组腺病毒载体的构建及其对骨肿瘤细胞MG63迁移侵袭能力的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第25卷
期数:
2017年07期
页码:
11-15
栏目:
实验研究
出版日期:
2017-07-15

文章信息/Info

Title:
Construction of Recombinant Adenovirus Vectors Overexpressing Noggin Gene and Its Effect on the Migration and Invasion Ability of Bone Tumor Cells MG63
文章编号:
1005-0205(2017)07-0011-05
作者:
张韬1 翁艳2 陈冬冬2 周燕芸2 肖莉莉2 张怡元2△
1.厦门大学附属福州第二医院骨科研究所(福州,350007)
2.厦门大学附属福州第二医院骨一区
△.通信作者 E-mail:zhangyiyuan_fj@126.com
Author(s):
ZHANG Tao1 WENG Yan2 CHEN Dongdong2 ZHOU Yanyun2 XIAO Lili2 ZHANG Yiyuan2△
1.Orthopaedic Institute, Fuzhou Second Hospital, Affiliated to Xiamen University, Fuzhou 350007, China;
2.The Frist Department of Orthopedics, Fuzhou Second Hospital, Affiliated to Xiamen University, Fuzhou 350007, China.
关键词:
Noggin基因;  过表达重组腺病毒;  骨肿瘤细胞MG63;  迁移;  侵袭
Keywords:
Noggin gene;  overexpressing recombinant adenovirus;  bone tumor cells MG63;  migration;  invasion
分类号:
R-33
文献标志码:
A
摘要:
目的:研究Noggin基因对骨肿瘤细胞MG63中Noggin,BMP-2,血管内皮生长因子(VEGF)及结缔组织生长因子(CTGF)的表达及细胞迁移侵袭的影响,探讨Noggin基因在骨肿瘤的发生和发展过程中的作用机制。方法:通过构建Noggin基因过表达重组腺病毒载体,并将其转染到骨肿瘤细胞MG63中,采用RT-PCR和Western blot分别检测空白对照组、过表达重组腺病毒组中Noggin,BMP-2,VEGF和CTGF的mRNA水平和蛋白水平,Transwell细胞迁移和侵袭能力实验考察两组细胞的迁移和侵袭能力。结果:成功构建Noggin基因过表达重组腺病毒载体,将其转染到骨肿瘤细胞MG63后,Noggin的mRNA及蛋白表达水平显著升高,BMP-2,VEGF及CTGF的mRNA及蛋白表达水平显著降低,转染Noggin基因过表达重组腺病毒的骨肿瘤细胞MG63迁移和侵袭的能力明显下降。结论:过表达Noggin基因能成功抑制骨肿瘤细胞MG63的侵袭和转移,有望成为骨肿瘤基因治疗的新方法。
Abstract:
Objective: To investigate the effect of Noggin gene on the expression of Noggin, BMP-2, vascular endothelial growth factor(VEGF)and connective tissue growth factor(CTGF)and the cell migration and invasion in bone tumor cells MG63, and to explore the mechanism of Noggin gene in the development and progression of bone tumors. Methods:The Noggin gene overexpressing recombinant adenovirus vectors were constructed and transfected into bone tumor cells MG63. The mRNA and protein levels of Noggin, BMP-2, VEGF, and CTGF in the blank control group and the overexpressing recombinant adenovirus group were detected by RT-PCR and Western blot respectively, and the migration and invasion ability of cells in the two groups were validated by Transwell cell migration and invasion ability experiment. Results:After the Noggin overexpressing recombinant adenovirus vectors were successfully constructed and transfected into bone tumor cells MG63, the mRNA and protein expression of Noggin were increased significantly, the mRNA and protein expression of BMP-2, VEGF and CTGF were inhibited significantly, and the migration and invasion ability of bone tumor cells MG63 infected with Noggin gene overexpressing recombinant adenovirus was decreased significantly. Conclusion:Overexpression of Noggin gene can inhibit the invasion and metastasis of bone tumor cells MG63 successfully, which is expected to be a new method for gene therapy of bone tumors.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:福建省卫计委创新课题(2014-cxb-23)
福州市科技计划项目(2015-S-141-10)
福州市临床重点专科建设项目(骨科)
福建省自然科学基金面上项目(2016J01594)
更新日期/Last Update: 2017-07-15