[1]帅波 杨功旭 沈霖△ 杨艳萍 孙敏 马陈 徐晓娟 夏雪.加味青娥丸对激素性股骨头坏死小鼠局部1,25(OH)2D3/VDR mRNA/RAS信号转导通路的影响[J].中国中医骨伤科杂志,2017,25(07):1-5.
　SHUAI Bo,YANG Gongxu,SHEN Lin,et al.Effect of Modified Qing'e Pill on Local 1,25(OH)2D3/ VDR mRNA/RAS Signal Transduction Pathway in Steroid-induced Ischemic Necrosis of Femoral Head in Mice[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2017,25(07):1-5.
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加味青娥丸对激素性股骨头坏死小鼠局部1,25(OH)2D3/VDR mRNA/RAS信号转导通路的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第25卷
期数:
2017年07期
页码:
1-5
栏目:
实验研究
出版日期:
2017-07-15

文章信息/Info

Title:
Effect of Modified Qing'e Pill on Local 1,25(OH)2D3/ VDR mRNA/RAS Signal Transduction Pathway in Steroid-induced Ischemic Necrosis of Femoral Head in Mice
文章编号:
1005-0205(2017)07-0001-05
作者:
帅波1 杨功旭2 沈霖1△ 杨艳萍1 孙敏2 马陈1 徐晓娟1 夏雪1
1.华中科技大学同济医学院附属协和医院(武汉,430022)
2.湖北省中医院
△.通信作者 E-mail:shenlinhb@sina.com 本文帅波、杨功旭为共同第一作者
Author(s):
SHUAI Bo1;  YANG Gongxu2;  SHEN Lin1△;  YANG Yanping1;  SUN Min2;  MA Chen1;  XU Xiaojuan1;  XIA Xue1
1.Department of Integrated Traditional Chinese Medicine and Western Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;
2.Hubei provincial Hospital of Traditional Chinese Medicine,Wuhan 430074,China.
关键词:
激素性股骨头坏死;  青娥丸;  活性维生素D;  维生素D受体;  肾素-血管紧张素系统
Keywords:
steroid-induced ischemic necrosis of femoral head;  Qing'e pill;  1; 25(OH)2D3;  Vitamin D receptor(VDR);  Remin-Angiotensin system
分类号:
R-33
文献标志码:
A
摘要:
S信号转导通路的影响,以探讨加味青娥丸治疗激素性股骨头坏死的机制。方法:8周龄SPF 级C57BL/6雄性小鼠随机分5组,每组12只,即空白对照组(A组)、激素性骨坏死模型组(B组)、青娥丸加味组(C组)、骨化三醇干预组(D组)及血管紧张素转换酶抑制剂(ACEI)干预组(E组),治疗半年后,运用micro-CT检测并经三维重建获得股骨头颈局部骨组织微观结构,检测股骨头颈局部1,25(OH)2D3/VDR mRNA/RAS系统(ACE,AngⅡ mRNA)表达情况。结果:空白对照组(A组)骨体积分数(BV/TV)、骨小梁数目(Tb.N)、骨小梁厚度(Tb.Th)较模型组(B组)显著增加,差异有统计学意义(P<0.05),结构模型指数(SMI)、骨小梁间隙(Tb.Sp)、骨表面积体积比(BS/BV)较模型组(B组)明显降低,差异有统计学意义(P<0.05),模型组股骨头局部骨小梁稀疏,孔隙率增高,结构紊乱,部分骨小梁结构不完整,表明模型成功。治疗后C组、D组及E组1,25(OH)2D3,VDR mRNA表达均较模型组高,差异有统计学意义(P<0.05),而ACE及AngⅡ mRNA表达较模型组低,差异有统计学意义(P<0.05)。结论:加味青娥丸可能通过调节1,25(OH)2D3/VDR mRNA/RAS信号转导通路而对股骨头坏死疾病的进展起到一定的治疗作用。
Abstract:
Objective: To observe the effects of modified Qing'e Pill on the local signal transduction pathway of 1,25(OH)2D3/VDR mRNA/RAS in mice of steroid-induced ischemic necrosis of femoral head, and to explore the mechanism of modified Qing'e pill in the treatment of steroid induced necrosis of femoral head.Methods: All 60 SPF male C57BL/6 mice, 8-weeks-old, were randomly divided into 5 groups, 12 rats in each group: control group(group A), steroid induced osteonecrosis model group(group B), Qing'e pill group(group C), calcitriol intervention group(group D)and inhibitor of angiotensin converting enzyme(ACEI)intervention group(group E).Six months later, micro-CT detection and three-dimensional reconstruction were used to obtain the local bone tissue of femoral head, and the level of 1,25(OH)2D3, VDR mRNA and RAS system(ACE and AngⅡ mRNA)in the femoral head were detected.Results: The bone volume fraction(BV/TV), the number of trabeculae(Tb.N)and the thickness of the trabecular bone(Tb.Th)in the blank control group A increased significantly compared with the model group(group B)(P<0.05), but the structure model index(SMI), trabecular space(Tb.Sp)and bone surface area volume ratio(BS/BV)were significantly lower(P<0.05).In the model group, the local trabecular bone of the femoral head was sparse, the porosity was increased, the structure was disturbed and some trabecular structures were incomplete.The expressions of 1,25(OH)2D3 and VDR mRNA in group C, group D and group E were higher than those in model group(P<0.05).The expression of ACE and AngⅡ mRNA in group C, D and E was lower than that in model group(P<0.05).Conclusion: Modified Qing'e pill is effective for treating the steroid-induced ischemic necrosis of femoral head through the local signal transduction pathway of 1,25(OH)2D3/VDR mRNA/RAS.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(81403257,81473492,81273907)
更新日期/Last Update: 2017-07-15