[1]刘伯龄△ 梁珪清 李照辉 刘少强 吴彬彬 林鹏程.透骨消痛胶囊含药血清对软骨细胞TGF-β通路中相关信号分子影响的实验研究[J].中国中医骨伤科杂志,2016,24(12):1-6.
　LIU Boling LIANG Guiqing LI Zhaohui LIU Shaoqiang WU Binbin LIN Pengcheng.Effect of Touguxiaotong Capsule on Related Molecules in TGF-β Signal Pathway of Chondrocytes[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2016,24(12):1-6.
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透骨消痛胶囊含药血清对软骨细胞TGF-β通路中相关信号分子影响的实验研究
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第24卷
期数:
2016年12期
页码:
1-6
栏目:
实验研究
出版日期:
2016-12-05

文章信息/Info

Title:
Effect of Touguxiaotong Capsule on Related Molecules in TGF-β Signal Pathway of Chondrocytes
作者:
刘伯龄1△ 梁珪清1 李照辉1 刘少强1 吴彬彬1 林鹏程1
Author(s):
LIU Boling1△ LIANG Guiqing1 LI Zhaohui1 LIU Shaoqiang1 WU Binbin1 LIN Pengcheng1
1Department of Orthopaedic,Second Hospital of Fuzhou,Affiliated to Xiamen University,Fuzhou 350007,China.
关键词:
透骨消痛胶囊;  含药血清;  软骨细胞;  TGF-β信号通路
Keywords:
touguxiaotong capsule;  medicated serum;  chondrocytes;  TGF-β
分类号:
R-33
文献标志码:
A
摘要:
目的:观察透骨消痛胶囊含药血清对硝普钠造模后软骨细胞TGF-β(转化生长因子-β)信号通路中ALK1,ALK5,ALK1/ALK5及MMP-13的表达影响,探讨其防治骨关节炎的作用机制。方法:2月龄清洁级雄性SD大鼠90只,随机分为空白组30只、壮骨关节丸组30只和透骨消痛胶囊组30只,制备含药血清。4周龄清洁级雄性SD大鼠膝关节软骨建立软骨细胞体外培养体系。1 mmol/L SNP干预第2代软骨细胞复制软骨细胞凋亡模型。分为1 mmol/L SNP组,1 mmol/L SNP+10%壮骨关节丸组,1 mmol/L SNP+5%、10%和15%透骨消痛胶囊组,分别培养3,6和9 d,Real-time PCR和Western blot分别检测各组软骨细胞ALK1,ALK5及MMP-13基因及蛋白表达。结果:含药血清分别干预细胞3,6和9 d后,ALK1,ALK1/ALK5及MMP-13基因及蛋白的表达,壮骨关节丸组和透骨消痛胶囊组均明显低于1 mmol/L SNP组(P<0.01,P<0.05); ALK5基因及蛋白的表达,壮骨关节丸组和透骨消痛胶囊组均明显高于1 mmol/L SNP组(P<0.01,P<0.05)。结论:透骨消痛胶囊通过调节TGF-β信号通路发挥抗软骨细胞凋亡作用。
Abstract:
Objective:To investigate the effect of Touguxiaotong capsule medicated serum on the expression of ALK1,ALK5,ALK1/ALK5 and MMP-13 in model chondrocytes intervened by SNP,and to explore its mechanism of prevention and treatment of osteoarthritis.Methods:Ninety male SD rats(two months old)were randomly divided into blank group,Zhuangguguanjie pill group,and Touguxiaotong capsule group(n=30 in each group),preparing for the medicated serum respectively.Chondrocytes cultured in vitro,which was collected from knee joint cartilage of 4-weeks-old male SD rats,was established.1 mmol/L SNP was chosen to mould the degenerated chondrocytes model in passage 2 chondrocytes,which were divided as follows:1 mmol/L SNP group,1 mmol/L SNP+ 10% Zhuangguguanjie pill group,and 1 mmol/L SNP+ 5%,10% and 15% Touguxiaotong capsule group,intervened by 3 d,6 d and 9 d respectively.The expression of ALK1,ALK5,MMP-13 and related protein in the chondrocytes of each group were detected by RT-PCR and Western blot respectively.Results:Compared with 1 mmol/L SNP group,the expression of ALK1,ALK1/ALK5,MMP-13 and related protein in Zhuangguguanjie pill group and Touguxiaotong capsule group were significantly lower after treated with medicated serum at 3th,6th,and 9th day respectively(P<0.01,P<0.05),while the expression of ALK5 were significantly increased(P<0.01,P<0.05).Conclusion:Touguxiaotong capsule plays an anti-chondrocyte apoptosis effect by regulating the TGF-β signal pathway.

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备注/Memo

备注/Memo:
基金项目:福建省福州市科技计划项目(2013-S-123-8) 1厦门大学附属福州第二医院骨科(福州,350007) △通信作者 E-mail:liuboling2@126.com
更新日期/Last Update: 2016-12-01