[1]梁文清△ 周国芳 钱宇 徐国健 谢明华.多孔微球种子细胞储库型组织工程肌腱支架体外构建[J].中国中医骨伤科杂志,2016,24(01):9-12.
　LIANG Wenqing ZHOU Guofang QIAN Yu XU Guojian XIE Minghua.Porosity Microsphere as Seed Cells Storage to Build Tissue Engineering Tendon Scaffold in Vitro[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2016,24(01):9-12.
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多孔微球种子细胞储库型组织工程肌腱支架体外构建
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第24卷
期数:
2016年01期
页码:
9-12
栏目:
实验研究
出版日期:
2016-01-13

文章信息/Info

Title:
Porosity Microsphere as Seed Cells Storage to Build Tissue Engineering Tendon Scaffold in Vitro
文章编号:
1005-0205(2016)01-0009-04
作者:
梁文清1△ 周国芳1 钱宇1 徐国健1 谢明华1
1.浙江省绍兴市人民医院骨科(浙江 绍兴,312000)
△.通信作者 E-mail:chyygklwq@hotmail.com
Author(s):
LIANG Wenqing1△ ZHOU Guofang1 QIAN Yu1 XU Guojian1 XIE Minghua1
1.Department of Orthopedics, Shaoxing People's Hospital, Shaoxing 312000, Zhejiang China.
关键词:
多孔微球;  骨髓间充质干细胞;  同种异体肌腱;  组织工程
Keywords:
porosity microsphere;  bone marrow mesenchymal stem cells;  acellular allogenic tendon;  tissue engineering
分类号:
R-33
文献标志码:
A
摘要:
目的:以多孔微球作为种子细胞储库,构建体外组织工程肌腱支架。方法:分离、培养大鼠骨髓间充质干细胞(BMSCs)作为种子细胞,复乳法制备多孔微球并接种种子细胞。编织去细胞肌腱支架,并测定其力学特征及孔隙率。将种子细胞微球储库接种在编织去细胞肌腱支架上,测定胶原I,Ⅲ(Collagen I,Ⅲ)的基因表达。结果:BMSCs细胞可以进行成骨和成纤维分化,在多孔微球内可生长增殖。编织去细胞肌腱支架有较好的力学特征及较高的孔隙率。将种子细胞微球储库接种在编织去细胞肌腱支架上后,与对照组相比可以分泌更多的Collagen I,Ⅲ.结论:种子细胞微球储库型编织去细胞肌腱组织工程支架保留了较好的组织学形态及生物学特性。
Abstract:
Objective: To choose porosity microsphere as seed cells storage, and to build tissue engineering tendon scaffold in vitro.Methods: The bone marrow mesenchymal stem cells(BMSCs)of rats were isolate and culture as seed cell.The porosity microsphere was prepared by expanding foam method, and then it was implanted into seed cells.The acellular allogenic tendon was weaved and its porosity and mechanical strength change were analyzed.The BMSCs storage of porosity microsphere were inoculatein into weaved acellular allogenic tendon, and the gene expression of Collagen I, Ⅲ were detected.Results: BMSCs cell could differentiate into osteoblast cells and fibroblast cells, and solidly grow interiorly in porosity microsphere.The acellular allogenic tendon scaffold has the better mechanics property and high porosity.BMSCs can secrete more Collagen I, Ⅲ when combined cultured with tissue engineering scaffold.Conclusion: The acellular allogenic tendon scaffold with porosity microsphere as seed cells storage reserve histology and biomechanics property.

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备注/Memo

备注/Memo:
收稿日期:2015-03-07
基金项目:浙江省自然科学基金资助项目(LY13H060005)
浙江省公益性技术应用研究计划项目(2014C33254)
浙江省医药卫生省部共建培育计划项目(2015PYA012)
绍兴市公益性应用计划项目(2013B70081)
更新日期/Last Update: 2016-01-05