[1]徐逸生△,张秋平,杨仁轩,等.金桂外洗方对完全弗氏佐剂性炎症痛小鼠血清 及局部组织PGE2浓度的影响[J].中国中医骨伤科杂志,2015,23(10):5-7.
　XU Yisheng,ZHANG Qiuping,YANG Renxuan,et al.Effect of Jingui Waixi Decoction on PGE2 Concentration in Serum and Local Tissue in Complete Freund's Adjuvant Induced Inflammatory Pain Mice Model[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2015,23(10):5-7.
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金桂外洗方对完全弗氏佐剂性炎症痛小鼠血清 及局部组织PGE2浓度的影响
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第23卷
期数:
2015年10期
页码:
5-7
栏目:
实验研究
出版日期:
2015-10-01

文章信息/Info

Title:
Effect of Jingui Waixi Decoction on PGE2 Concentration in Serum and Local Tissue in Complete Freund's Adjuvant Induced Inflammatory Pain Mice Model
文章编号:
1005-0205（2015）10-0005-03
作者:
徐逸生1△; 张秋平2; 杨仁轩1; 曹学伟1; 刘金文1
1广东省中医院骨三科（广州，510120）
2广州中医药大学中药学院
Author(s):
XU Yisheng1△; ZHANG Qiuping2; YANG Renxuan1; CAO Xuewei1; LIU Jinwen1
1Department of Third Orthopeadic, Guangdong Hospital of Tradtional Chinese Medicine,,Guangzhou 510120, China;
2College of Chinese Pharmaceutical Science, Guangzhou University of Tradtional Chinese Medicine,Guangzhou 510405,China.
关键词:
金桂外洗方; 外用; 炎症; 前列腺素E2(PGE2)
Keywords:
Jingui Waixi decoction; external usage; inflammation; PGE2
分类号:
R 33
文献标志码:
A
摘要:
目的：研究金桂外洗方对完全弗氏佐剂性炎症痛小鼠血清和局部前列腺素E2(PGE2)浓度的影响。方法：将体质量180~200 g SPF级雌雄各20只KM小鼠在右后脚掌以完全弗氏佐剂法建立炎症模型，随机分为金桂外洗方外洗组（中药组）、扶他林溶液外洗组（西药组）、金桂外洗方+扶他林溶液外洗组（中西组）、造模组（阳性组）和空白组，1周后取小鼠血清和局部组织进行ELISA来测定PGE2.结果：1）血清PGE2浓度：阳性组＞西药组＞中药组＞中西组＞空白组，阳性组和西药组、中药组和中西组、中西组与空白组组间比较，组间差异有统计学意义（P＜0.01）。中药组、西药组、中西组、阳性组和空白组血清PGE2浓度分别是（1.02±0.20）pg/mL，（1.11±0.17）pg/mL，（0.85±0.15）pg/mL，（1.65±0.11）pg/mL，（0.56±0.14）pg/mL.2）局部组织PGE2浓度：阳性组＞西药组＞中药组＞中西组＞空白组。中西组与空白组组间比较，组间差异无统计学意义（P＞0.05）；阳性组与西药组、西药组与和中药组、中药组和中西组组间比较，组间差异有统计学意义（P＜0.01）。中药组、西药组、中西组、阳性组和空白组局部组织PGE2浓度分别是（130.19±17.00）pg/mL，（210.29±14.50）pg/mL，（65.79±17.30）pg/mL，（788.00±95.15）pg/mL，（37.77±7.48）pg/mL.结论：金桂外洗方发挥消炎止痛的作用途径是通过降低血清和局部组织PGE2的浓度来实现，较扶他林作用明显。
Abstract:
Objective: To investigate the effect of Jingui Waixi decoction on PGE2 concentration in serum and local tissue in complete Freund's adjuvant (CFA) induced inflammatory pain mice model. Methods: CFA inflammation rats model was set up in the right rear foot of 180~200 g SPF level KM mice which were 20 male and 20 female respectively. Mice were randomly divided into five groups, which were Jingui Waixi decoction washed group (Chinese traditional medicine group, TCM group), voltaren washed group (modern medicine group), Jingui Waixi decoction added voltaren washed group (combination group), CFA model group (positive group) and saline treated group (blank group). The PGE2 concentration of serum and local tissue was detected by ELISA kit one week after treatment. Results: 1)The serum PGE2 concentration were 1.02±0.20, 1.11±0.17, 0.85±0.15, 1.65±0.11, and 0.56±0.14 (pg/mL) in the TCM group, modern medicine group, combination group, positive group, and blank group respectively. The concentration was followed by positive group>modern medicine group>TCM group> combination group>blank group. The differences between positive group and modern medicine group, TCM group and combination group, combination group and blank group were statistically significant (P＜0.01). 2)The local tissue PGE2 concentration were 130.19±17.00, 210.29±14.50, 65.79±17.30, 788.00±95.15, and 37.77±7.48 (pg/mL) in the TCM group, modern medicine group, combination group, positive group, and blank group respectively. The concentration was followed by positive group>modern medicine group>TCM group> combination group>blank group. There was no significant difference between the combination group and the blank group (P＞0.05); The differences between positive group and modern medicine group, modern medicine group and TCM group, TCM group and combination group werre statistically significant (P＜0.01). Conclusion: The mechanism of Jingui Waixi decoction for inhibiting inflammation and pain is though reducing PGE2 concentration both in serum and local tissue, and the effect is better than voltaren.

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备注/Memo

备注/Memo:
收稿日期：2015-01-11
基金项目：建设中医药强省立项课题（20132171）
广东省科技厅科研基金资助（2011B031700021）
1广东省中医院骨三科（广州，510120）
2广州中医药大学中药学院

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更新日期/Last Update: 2017-09-08