[1]赵永见,薛纯纯,王利波,等.益气化瘀方缓解关节不稳型小鼠膝骨关节炎的实验研究[J].中国中医骨伤科杂志,2015,23(06):1-4.
　ZHAO Yongjian,XUE Chunchun,WANG Libo,et al.Research of Yiqihuayu Recipe on Knee Osteoarthritis of Joint Instability Type in Mice[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2015,23(06):1-4.
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益气化瘀方缓解关节不稳型小鼠膝骨关节炎的实验研究
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第23卷
期数:
2015年06期
页码:
1-4
栏目:
实验研究
出版日期:
2015-06-15

文章信息/Info

Title:
Research of Yiqihuayu Recipe on Knee Osteoarthritis of Joint Instability Type in Mice
文章编号:
1005-0205(2015)06-0001-04
作者:
赵永见1 2;  薛纯纯1 2;  王利波1 2;  李晓锋1 2;  施杞1 2;  王拥军1 2;  舒冰1 2△
1.上海中医药大学附属龙华医院(上海,200032)
2.上海中医药大学脊柱病研究所
△.通信作者 E-mail:shubing523@hotmail.com
Author(s):
ZHAO Yongjian1; 2;  XUE Chunchun1; 2;  WANG Libo1; 2;  LI Xiaofeng1; 2; SHI Qi1; 2;  WANG Yongjun1; 2;  SHU Bing1; 2
1.Longhua Hospital, Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
2.Spine Research Institute, Shanghai University of Traditional Chinese Medicine
关键词:
膝骨关节炎;  益气化瘀方;  软骨退变
Keywords:
Knee osteoarthritis;  Yiqihuayu recipe;  Cartilage degeneration
分类号:
R-33
文献标志码:
A
摘要:
目的:观察益气化瘀方是否能够缓解关节不稳诱导的小鼠膝骨关节炎病理改变,初步探讨疗效机制。方法:取12周龄C57BL/6小鼠30只,分为假手术组、模型组和益气化瘀方组,模型组和益气化瘀方组左膝关节行内侧半月板及前交叉韧带离断术诱导膝骨关节炎,并分别給予生理盐水和益气化瘀方煎剂灌胃。8周后取左侧膝关节,Micro-CT扫描后进行三维重建、阿尔辛兰/橙黄G染色以及Ⅱ型胶原免疫组织化学染色,观察膝关节软骨病理改变。结果:术后8周,模型组小鼠左侧膝关节内侧半月板钙化明显增加,关节软骨边缘骨赘形成,关节软骨表面不平整,出现破裂,阿尔辛兰和Ⅱ型胶原染色层变薄; 益气化瘀方组小鼠膝关节稍有软骨边缘骨赘形成和半月板钙化,但无模型组显著,关节软骨表面稍有不平整,未出现破裂,阿尔辛兰和Ⅱ型胶原染色层稍有变薄,但无模型组显著。结论:益气化瘀方可以减轻半月板和前交叉韧带离断诱导的小鼠膝骨关节炎病理改变。
Abstract:
Objective:To observe the curative effect and pathological changes of Yiqihuayu recipe on the knee osteoarthritis of joint instability type in mice. Methods:All 30 C57BL/6 mice of 12 weeks were randomly divided into sham group, model group and Yiqihuayu group. Mice in model group and Yiqihuayu group were performed of anterior cruciate ligament transection and partial medial menisectomy in left knee joints to induce knee osteoarthritis, and were treated with saline and Yiqihuayu recipe respectively. Eight weeks after-treatment, the left knee joint was harvested. Micro-CT scanning and reconstruction was performed to observe the structure of knee joints. Alcian blue/orange G staining was performed to reveal the pathological changes of knee joints. Immunohistochemistry(IHC)staining was performed to detect the changes of type Ⅱ collagen.Results:Eight weeks after the surgery, medial meniscus ossification of the left knee joint was developed and osteophyte at the edge of articular cartilage was formed in model group. The cartilage surface was unsmooth with little cracks and reduced alcian blue and type Ⅱ collagen staining. In Yiqihuayu group, medial meniscus ossification and osteophyte formation were partially decreased and the cartilage destruction was improved. Conclusion:Yiqihuayu recipe could alleviate the pathological changes of knee osteoarthritis in mice model of joint instability.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-01-01
基金项目:国家自然科学基金青年基金(81403239)
上海市自然科学基金青年项目(12ZR1450400)
上海高校青年教师培养资助计划(ZZSZY12064)
教育部“创新团队”发展计划(IRT1270)
上海中医药大学“085工程”中医药学一流学科建设项目(085ZY1204)
上海高校实验技术队伍建设计划(LH01.24.001)
更新日期/Last Update: 2015-06-10