[1]史铭钰 黄玮.当归多糖对激素性股骨头坏死大鼠骨代谢的影响[J].中国中医骨伤科杂志,2026,34(05):18-24.[doi:10.20085/j.cnki.issn1005-0205.260503]
　SHI Mingyu,HUANG Wei.Effect of Angelica Polysaccharide on Bone Metabolism in Rats with Hormonal Femoral Head Necrosis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2026,34(05):18-24.[doi:10.20085/j.cnki.issn1005-0205.260503]
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当归多糖对激素性股骨头坏死大鼠骨代谢的影响()

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第34卷
期数:
2026年05期
页码:
18-24
栏目:
基础研究
出版日期:
2026-05-10

文章信息/Info

Title:
Effect of Angelica Polysaccharide on Bone Metabolism in Rats with Hormonal Femoral Head Necrosis
文章编号:
1005-0205(2026)05-0018-07
作者:
史铭钰 黄玮
1常州市中医医院(江苏 常州,213000)
Author(s):
SHI Mingyu1; HUANG Wei1△
1Changzhou Hospital of Traditional Chinese Medicine,Changzhou 213000,Jiangsu China.
关键词:
当归多糖;  激素性股骨头坏死大鼠;  骨代谢;  股骨头坏死
Keywords:
angelica polysaccharide;  hormonal femoral head necrosis rats;  bone metabolism;  femoral head necrosis
分类号:
R285.5
DOI:
10.20085/j.cnki.issn1005-0205.260503
文献标志码:
A
摘要:
目的:基于腺苷酸活化蛋白激酶(AMPK)/哺乳动物雷帕霉素靶蛋白(mTOR)信号通路探究当归多糖对激素性股骨头坏死大鼠骨代谢的影响。方法:将大鼠分为对照组、模型组、低当归多糖组、中当归多糖组、高当归多糖组、当归多糖+抑制剂组,每组12只,除对照组外的其余5组均通过注射脂多糖与甲泼尼龙诱导激素性股骨头坏死大鼠模型。显微CT扫描分析大鼠骨密度(BMD)、骨体积分数(BV/TV)、骨小梁厚度(Tb.Th)和骨小梁数(Tb.N); 苏木精-伊红(HE)染色观察股骨病理形态; 抗酒石酸酸性磷酸酶(TRAP)染色观察破骨细胞; 免疫组织化学染色分析骨代谢相关蛋白表达; 二氢乙锭(DHE)染色检测活性氧(ROS)水平; Western Blot法检测大鼠股骨AMPK/mTOR通路蛋白表达。结果:与对照组相比,模型组大鼠股骨头骨密度、BV/TV、Tb.Th和Tb.N均降低,软骨面积和骨小梁面积减少,存在大量空陷窝,骨表面破骨细胞数增加,Osterix、Runx相关转录因子2(RUNX2)和骨钙素(OCN)阳性细胞数减少,活性氧水平和mTOR蛋白表达升高,p-AMPK蛋白表达降低,差异有统计学意义(P<0.05); 与模型组相比,低/中/高当归多糖组大鼠股骨头骨密度、BV/TV、Tb.Th和Tb.N均升高,骨小梁面积增加,空陷窝减少,骨表面破骨细胞数减少,Osterix、RUNX2和骨钙素阳性细胞数增加,活性氧水平和mTOR蛋白表达降低,p-AMPK蛋白表达升高,差异有统计学意义(P<0.05); 与高当归多糖组相比,当归多糖+抑制剂组大鼠股骨头骨密度、BV/TV、Tb.Th和Tb.N均降低,骨小梁面积减少,空陷窝增加,骨表面破骨细胞数增加,Osterix、RUNX2和骨钙素阳性细胞数减少,活性氧水平和mTOR蛋白表达升高,p-AMPK蛋白表达降低,差异有统计学意义(P<0.05)。结论:当归多糖通过调节AMPK/mTOR信号通路增强激素性股骨头坏死大鼠骨代谢。
Abstract:
Objective:To explore the effect of angelica polysaccharide on bone metabolism in rats with hormonal femoral head necrosis based on the AMP-activated protein kinase(AMPK)/mammalian target of rapamycin(mTOR)signaling pathway.Methods:In this study,the rats were divided into control group,model group,L-angelica polysaccharide group,M-angelica polysaccharide group,H-angelica polysaccharide group,and angelica polysaccharide + inhibitor group,with 12 per group.Except for the control group,the remaining 5 groups were injected with lipopolysaccharides and methylprednisolone to induce steroid-induced femoral head necrosis in rat models.Micro-CT scans were used to analyze rat bone mineral density(BMD),bone volume/tissue volume(BV/TV),and trabecular thickness(Tb.Th),and the number of trabecules(Tb.N).HE staining was used to observe the pathological morphology of the femur.TRAP staining was performed to identify osteoclasts.Immunohistochemical staining was implemented to explore the expression of bone metabolism-related proteins.DHE staining was used to measure reactive oxygen species(ROS)level.Moreover,Western Blot was implemented to measure the expression of AMPK/mTOR pathway proteins in the femoral tissue of rats.Results:Compared with the control group,the model group showed a decrease in BMD,BV/TV,Tb.Th,and Tb.N of the femoral head in rats,a reduction in cartilage area and trabecular area,and a large number of empty cavities,the number of osteoclasts on the bone surface increased,the numbers of Osterix,Runx related transcription factor 2(RUNX2),and osteocalcin(OCN)positive cells decreased,the ROS level and mTOR protein expression increased,while p-AMPK protein expression decreased(P<0.05).Compared with the model group,the L-angelica polysaccharide group,M-angelica polysaccharide group,and H-angelica polysaccharide group showed an increase in BMD,BV/TV,Tb.Th,and Tb.N of the femoral head in rats,an increase in trabecular area,a decrease in empty cavities,a decrease in the number of osteoclasts on the bone surface,a raise in Osterix,RUNX2,and OCN positive cells,a decrease in ROS level and mTOR protein expression,and a raise in p-AMPK protein expression(P<0.05).Compared with the H-angelica polysaccharide group,the angelica polysaccharide+inhibitor group showed a decrease in BMD,BV/TV,Tb.Th,and Tb.N in the femoral head in rats,a decrease in trabecular area,a raise in empty cavities,an increase in the number of osteoclasts on the bone surface,a decrease in Osterix,RUNX2,and OCN positive cells,an increase in ROS level and mTOR protein expression,and a decrease in p-AMPK protein expression(P<0.05).Conclusion:Angelica polysaccharide enhances bone metabolism in rats with hormonal femoral head necrosis by regulating the AMPK/mTOR signaling pathway.

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(收稿日期:2025-09-19)

备注/Memo

备注/Memo:
基金项目:2024年常州市孟河医派传承创新发展项目(MH202414)
△通信作者 E-mail:huangwei312@126.com
更新日期/Last Update: 2026-05-15